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Age-Related Changes in the Yeast Component of the Drosophila melanogaster Microbiome

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Abstract—

Drosophila melanogaster fruit flies are an important model for studying the multifaceted interactions between a multicellular organism and its microbiome. The nature of these interactions is largely determined by the regular changes in abundance and composition of the microbiome that occur during the host’s life. The currently available data on age- and life cycle stage-related changes in the Drosophila microbiome relate mainly to its bacterial component, while little is known about such changes in the equally important yeast component. The present work describes the quantitative and qualitative composition of the yeast component of the D. melanogaster microbiome in three laboratory lines, reared under different conditions, at four developmental stages: late larvae and adults aged 1, 7, and 14 days after eclosion. In all three lines, the total yeast abundance changed similarly with the age of insects, with the highest and lowest yeast counts in 7- and 1-day adults, respectively. In the fly lines reared on moderately unfavorable substrates supplemented with 2 and 4% NaCl, the abundance and species diversity of yeasts at all four developmental stages was higher than in the flies reared on a standard (favorable) food substrate. Our results indicate the inconstancy of the yeast component of the D. melanogaster microbiome and its regular changes with the insect’s age, which must be taken into account when studying the relationships between symbiotic yeasts and their hosts.

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Funding

The work was supported by the Russian Foundation for Basic Research, projects nos. 19-34-90141 and 18-04-00915).

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Authors and Affiliations

  1. Moscow State University, 119991, Moscow, Russia

    A. S. Dmitrieva, I. A. Maksimova, A. V. Kachalkin & A. V. Markov

  2. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia

    A. V. Kachalkin

  3. Borissiak Paleontological Institute, Russian Academy of Sciences, 117997, Moscow, Russia

    A. V. Markov

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  1. A. S. Dmitrieva
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Correspondence to A. S. Dmitrieva.

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The authors declare that they have no conflict of interest. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Dmitrieva, A.S., Maksimova, I.A., Kachalkin, A.V. et al. Age-Related Changes in the Yeast Component of the Drosophila melanogaster Microbiome. Microbiology 90, 229–236 (2021). https://doi.org/10.1134/S0026261721020028

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