Skip to main content
SpringerLink
Log in

Diversity of Planktonic Bacteria in Durgun and Taishir Reservoirs (Western Mongolia)

  • EXPERIMENTAL ARTICLES
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract—

This is the first report on the application of high-throughput sequencing of the 16S rRNA gene fragments for investigation of bacterioplankton diversity in two largest and relatively young reservoirs of Mongolia—Taishir, formed on the Zavkhan River, and Durgun, formed on the Chono Harayh Channel. A total of 145 phylotypes (with a cluster distance of 0.03) belonging to 18 phyla were identified in the communities. The share of OTUs that were not identified at the phylum level varied in the range of 0.6–3.0%. According to the ACE and CHAO1 diversity indices, bacterioplankton species richness was highest in the hypertrophic section of the Chono Harayh channel below the dam of the Durgun Hydropower Station and lowest in the mesotrophic section of the Zavkhan River above the Taishir Reservoir. According to the Shannon and Simpson indices, the species abundance of different species in the community characterized the mesotrophic and eutrophic pelagic zones of the Taishir and Durgun reservoirs, respectively, as areas with the most equality. The reservoir systems differed significantly in taxonomic diversity, as evidenced by the ratio of common and unique OTUs, but their representation was not uniform along the rivers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

Similar content being viewed by others

REFERENCES

  1. Achtman, M. and Wagner, M., Microbial diversity and the genetic nature of microbial species, Nat. Rev. Microbiol., 2008, vol. 6, pp. 431–440.

    Article  CAS  Google Scholar 

  2. Avila, M.P., Staehr, P.A., Barbosa, F.A.R., Chartone-Souza, E., and Nascimento, A.M.A., Seasonality of freshwater bacterioplankton diversity in two tropical shallow lakes from the Brazilian Atlantic Forest, FEMS Microbiol. Ecol., 2017, vol. 93, art. fiw218.

    Article  Google Scholar 

  3. Azam, F., Cho, B.C., Smith, D.C., and Simon, M., Bacterial cycling of matter in the pelagic zone of aquatic ecosystems, in Large Lakes—Ecological Structure and Function, Tilzer, M.M. and Serruya, C., Eds., Berlin: Springer-Verlag, 1990, pp. 477–488.

    Google Scholar 

  4. Berdjeb, L., Ghiglione, J.F., Domaizon, I., and Jacquet, S., A 2-year assessment of the main environmental factors driving the free-living bacterial community structure in Lake Bourget (France), Microb. Ecol., 2011, vol. 61, pp. 941–954.

    Article  Google Scholar 

  5. Boulund, F., Computational methods for analysis of fragmented sequence data, PhD Thesis, Göteborg: University of Göteborg, 2015.

  6. Cole, J.R., Wang, Q., Cardenas, E., Fish, J., Chai, B., Farris, R.J., Kulam-Syed-Mohideen, A.S., McGarrell, D.M., Marsh, T., Garrity, G.M., and Tiedje, J.M., The Ribosomal Database Project: improved alignments and new tools for rRNA analysis, Nucleic Acids Res., 2009, vol. 37, pp. 141–145.

    Article  Google Scholar 

  7. Eiler, A., Zaremba-Niedzwiedzka, K., Martinez-Garcia, M., McMahon, K.D., Stepanauskas, R., Andersson, S.G., and Bertilsson, S., Productivity and salinity structuring of the microplankton revealed by comparative freshwater metagenomics, Environ. Microbiol. 2014, vol. 16, pp. 2682–2698.

    Article  CAS  Google Scholar 

  8. Fortunato, C.S., Herfort, L., Zuber, P., Baptista, A.M., Crump, B.C., Spatial variability overwhelms seasonal patterns in bacterioplankton communities across a river to ocean gradient, ISME J., 2012, vol. 6, pp. 554–563.

    Article  CAS  Google Scholar 

  9. Galach’yants, A.D., Bel’kova, N.L., Sukhanova, E.V., Galach’yants, Yu.P., Morozov, A.A., and Parfenova, V.V., Taxonomic composition of Lake Baikal bacterioneuston communities, Microbiology (Moscow), 2017, vol. 86, pp. 241–249.

    Article  Google Scholar 

  10. Gracas, D.A., Miranda, P.R., Barauna, R.A., McCulloch, J.A., Ghilardi, R., Jr., Schneider, M.P.C., and Silva, A., Microbial diversity of an anoxic zone of a hydroelectric power station reservoir in Brazilian Amazonia, Microb. Ecol., 2011, vol. 62, pp. 853–861.

    Article  Google Scholar 

  11. Jia, Y. and Whalen, J.K., A new perspective on functional redundancy and phylogenetic niche conservatism in soil microbial communities, Pedosphere, 2020, vol. 30, pp. 18–24.

    Article  Google Scholar 

  12. Kato, K., Bacteria—a link among ecosystem constituents, Res. Popul. Ecol., 1996, vol. 38, pp. 185–190.

    Article  Google Scholar 

  13. Kaushik, R., Balasubramanian, R., and Dunstan, H., Microbial quality and phylogenetic diversity of fresh rainwater and tropical freshwater reservoir, PLoS One, 2014, vol. 9, art. e100737.

    Article  Google Scholar 

  14. Kolmakova, O.V., Gladyshev, M.I., Trusova, M.Y., Rozanov, A.S., and Peltek, S.E., Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing, FEMS Microbiol. Ecol., 2014, vol. 89, pp. 442–450.

    Article  CAS  Google Scholar 

  15. Kosolapov, D.B. and Kosolapova, N.G., Pico- and nanoplankton in aquatic ecosystems in the Valley of the Lakes and Great Lakes Depression (Mongolia), Inland Water Biol-., 2018, vol. 11, pp. 269–277. https://doi.org/10.1134/S1995082918030094

    Article  Google Scholar 

  16. Krylov, A.V., Solongo, D., and Mendsaikhan, B., Zooplankton of the Durgun and the Taishir reservoirs (Western Mongolia) by the end of impoundment period, Arid Ecosystems, 2014, vol. 4. no. 2, pp. 85–90.

    Article  Google Scholar 

  17. Lebedeva, N.V., Krivolutskii, D.A., Puzachenko, Yu.G., D’yakonov, K., Aleshchenko, G.N., Smurnov, A.V., Maksimov, V.N., Tikunov, V.S., Ogureeva, G.N., and Kotova, T.V., Geografiya i monitoring bioraznoobraziya (Geography and Monitoring of Biodiversity), Kasimov, N.S., Romanova, E.P., and Tishkov, A.A., Eds., Moscow: Izd. Nauchnogo i Uchebno-Metod. Tsentra, 2002.

    Google Scholar 

  18. Lee, S.-Y. and Eom, Y.-B., Analysis of microbial composition associated with freshwater and seawater, Biomed. Sci. Lett., 2016, vol. 22, pp. 150–159.

    Article  Google Scholar 

  19. Li, J., Chen, C., Lu, J., Lei, A., and Hu, Z., Temporal patterns in bacterioplankton community composition in three reservoirs of similar trophic status in Shenzhen, China, Int. J. Environ. Res. Public Health, 2016, vol. 13, pii. E599. https://doi.org/10.3390/ijerph13060599

  20. Limolino, M.V., Riddle, B.R., and Brown, J.H., Biogeography, Sunderland, MA: Sinauer Associates, 2006, 3rd edn.

    Google Scholar 

  21. Lindström, E.S., Feng, X.M., Graneli, W., and Kritzberg, E.S., The interplay between bacterial community composition and the environment determining function of inland water bacteria, Limnol. Oceanogr., 2010, vol. 55, pp. 2052–2060.

    Article  Google Scholar 

  22. Llirós, M., Inceoğlu, Ö., García-Armisen, T., Anzil, A., Leporcq, B., Pigneur, L.-M., Viroux, L., Darchambeau, F., Descy, J.-P., and Servais, P., Bacterial community composition in three freshwater reservoirs of different alkalinity and trophic status, PLoS One, 2014, vol. 9, art. e116145. https://doi.org/10.1371/journal.pone.0116145

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Logares, R., Haverkamp, T.H.A., Kumar, S., Lanzén, A., Nederbragt, A.J., Quince, C., and Kauserud, H., Environmental microbiology through the lens of high-throughput DNA sequencing: synopsis of current platforms and bioinformatics approaches, J. Microbiol. Meth., 2012, vol. 91, pp. 106–113.

    Article  CAS  Google Scholar 

  24. Lymperopoulou, D.S., Kormas, K.A., and Karagouni, A.D., Variability of prokaryotic community structure in a drinking water Reservoir (Marathonas, Greece), Microbes Environ., 2012, vol. 27, pp. 1–8.

    Article  Google Scholar 

  25. Ma, L., Mao, G., Liu, J., Gao, G., Zou, C., Bartlam, M.G., and Wang, Y., Spatial-temporal changes of bacterioplankton community along an exhorheic river, Front. Microbiol., 2016, vol. 7, art. 250. https://doi.org/10.3389/fmicb.2016.00250

    Article  PubMed  PubMed Central  Google Scholar 

  26. Matishov, D.G., Stakheev, V.V., Glushchenko, G.Yu., and Chirak, E.L., Metagenomic analysis of the bacterial community of the Sea of Azov, Oceanology, 2015, vol. 55, pp. 696–700.

    Article  Google Scholar 

  27. Newton, R.J., Jones, S.E., Eiler, A., McMahon, K.D., and Bertilsson, S., A guide to the natural history of freshwater lake bacteria, Microbiol. Mol. Biol. Rev., 2011, vol. 75, pp. 14–49.

    Article  CAS  Google Scholar 

  28. Pedrós-Alió, C., The rare bacterial biosphere, Ann. Rev. Mar. Sci., 2012, vol. 4, pp. 449–466.

    Article  Google Scholar 

  29. Qu, J., Jia, C., Liu, Q., Li, Z., Liu, P., Yang, M., Zhao, M., Li, W., Zhu, H., and Zhang, Q., Dynamics of bacterial community diversity and structure in the terminal reservoir of the South-to-North water diversion project in China, Water, 2018, vol. 10, art. 709.

    Article  Google Scholar 

  30. Radnagurueva, A.A., Lavrentieva, E.V., Budagaeva, V.G., Barkhutova, D.D., Namsaraev, B.B., and Dunaevsky, Y.E., Organotrophic bacteria of the Baikal Rift Zone hot springs, Microbiology (Moscow), 2016, vol. 85, pp. 367–378.

    Article  CAS  Google Scholar 

  31. Ruiz-Gonzalez, C., Proia, L., Ferrera, I., Gasol, J.M., and Sabater, S., Effects of large river dam-regulation on bacterioplankton community structure, FEMS Microb. Ecol., 2013, vol. 84, pp. 316–331.

    Article  CAS  Google Scholar 

  32. Ruiz-Gonzalez, C., Salazar, G., Logares, R., Proia, L., Gasol, J.M., and Sabater, S., Weak coherence in abundance patterns between bacterial classes and their constituent OTUs along a regulated river, Front. Microbiol., 2015, vol. 6, art. 1293.

    Article  Google Scholar 

  33. Simek, K., Armengol, J., Comerma, M., Garcia, J.C., Kojecka, P., Nedoma, J., and Hejzlar, J., Changes in the epilimnetic bacterial community composition, production, and protist-induced mortality along the longitudinal axis of a highly eutrophic reservoir, Microb. Ecol., 2001, vol. 42, pp. 359–371.

    Article  CAS  Google Scholar 

  34. Simek, K., Hornak, K., Jezbera, J., Nedoma, J., and Znachor, P., Spatio-temporal patterns of bacterioplankton production and community composition related to phytoplankton composition and protistan bacterivory in a dam reservoir, Aquat. Microb. Ecol., 2008, vol. 51, pp. 249–262.

    Article  Google Scholar 

  35. Skopina, M.Yu., Pinevich, A.V., Vasileva, A.A., and Pershina, E.V., Diversity at low abundance: the phenomenon of the rare bacterial biosphere, Microbiology (Moscow), 2016, vol. 85, pp. 272–282.

    Article  CAS  Google Scholar 

  36. Sun, W., Xia, C., Xu, M., Guo, J., and Sun, G., Seasonality affects the diversity and composition of bacterioplankton communities in Dongjiang River, a drinking water source of Hong Kong, Front. Microbiol., 2017, vol. 8, art. 1644.

    Article  Google Scholar 

  37. Wang, Q., Garrity, G.M., Tiedje, J.M., and Cole, J.R., Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy, Appl. Environ. Microbiol., 2007, vol. 73, pp. 5261–5267.

    Article  CAS  Google Scholar 

  38. Yang, X., Huang, T., and Zhang, H., Effects of seasonal thermal stratification on the functional diversity and composition of the microbial community in a drinking water reservoir, Water, 2015, vol. 7, pp. 5525–5546.

    Article  CAS  Google Scholar 

Download references

Funding

This study was carried out within the framework of state task no. АААА-А18-118012690098-5. The field studies were supported by the Joint Russian–Mongolian complex biological expedition of the Russian Academy of Sciences and the Academy of Sciences of Mongolia.

Author information

Authors and Affiliations

  1. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, 152742, Borok, Russia

    E. V. Kuznetsova & D. B. Kosolapov

  2. Cherepovets State University, 162600, Cherepovets, Russia

    D. B. Kosolapov

  3. Laboratory of Microbiome and Microecology, Scientific Center for Family Health and Human Reproduction Problems, 664003, Irkutsk, Russia

    N. L. Belkova

Authors
  1. E. V. Kuznetsova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. B. Kosolapov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. L. Belkova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. V. Kuznetsova.

Ethics declarations

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. This article does not contain any studies involving animals or human subjects performed by any of the authors.

Additional information

Translated by D. Timchenko

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kuznetsova, E.V., Kosolapov, D.B. & Belkova, N.L. Diversity of Planktonic Bacteria in Durgun and Taishir Reservoirs (Western Mongolia). Microbiology 89, 595–602 (2020). https://doi.org/10.1134/S002626172005015X

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S002626172005015X

Keywords:

Search

Navigation