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Genetic Analysis of the Probiotic Strain Escheriсhia coli M-17 and the Potentially Probiotic Strain Escheriсhia coli ВМ

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Abstract—

Genomic sequences were studied for two Escheriсhiacoli strains: an industrial strain E. coli M-17 and a newly isolated high-technology strain E. coli BM. E. coli M-17 was confirmed to possess the species-specific biochemical profile; transmissive genes of antibiotic resistance, pathogenicity, and virulence, as well as integrated plasmids were not revealed. The sequence type of the strain was found to be ST141. E. coli ВM was found to possess decreased enzymatic activity. Its genome was found to contain determinants for antibiotic resistance (strA, strB, sul2, aadA1), several pathogenicity and virulence determinants, the Tn7 transposon, and fragments of incorporated plasmids (IncFIB and Col156). The strain was found to belong to the tenth sequence type, ST-10. These results indicate that E. coli strain M-17 satisfies all modern requirements to probiotic-producing strains, while due to genomic properties of E. coli ВM this strain may not be considered industrially promising for production of probiotic preparations, in spite of its good technological characteristics. Complete genome sequences of the strains E. coli M-17 and E. coli ВM were deposited to GenBank under accession nos. NZ_LBDD00000000 and NZ_LBDC00000000, respectively.

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Statement of the welfare of animals. This work did not involve any experiments in animal objects.

Conflict of interest. The authors declare that there exists no conflict of interests.

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Authors and Affiliations

  1. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Federal Service for Surveillance for Consumer Rights Protection and Human Welfare, 603000, Nizhny Novgorod, Russia

    I. V. Belova, A. G. Tochilina, I. V. Soloveva, E. I. Efimov, I. S. Gorlova, T. P. Ivanova & V. A. Zhirnov

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  1. I. V. Belova
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  2. A. G. Tochilina
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  3. I. V. Soloveva
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  4. E. I. Efimov
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  5. I. S. Gorlova
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  6. T. P. Ivanova
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  7. V. A. Zhirnov
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Correspondence to A. G. Tochilina.

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Translated by E. Babchenko

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Belova, I.V., Tochilina, A.G., Soloveva, I.V. et al. Genetic Analysis of the Probiotic Strain Escheriсhia coli M-17 and the Potentially Probiotic Strain Escheriсhia coli ВМ. Microbiology 88, 309–315 (2019). https://doi.org/10.1134/S0026261719030032

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  • DOI: https://doi.org/10.1134/S0026261719030032

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