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Anaerobic Methane Oxidation in Enrichment Cultures from Deep Sediments of a Mud Volcano Peschanka (South Baikal)

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Abstract

Under laboratory conditions, the microbial communities of bottom sediments of a mud volcano Peschanka (Lake Baikal) were found to carry out anaerobic methane oxidation (AOM). After 16 days of anaerobic cultivation of the enrichment cultures, methane content in the gas phase decreased, and microbial consortia were established. The content of carbon, nitrogen, and oxygen determined by energy dispersive X-ray spectroscopy (EDS) was higher than in the nearby sediment particles. The presence of bacteria of the NC10 phylum and archaea of the ANME-2d cluster was established by fluorescent in situ hybridization (FISH).

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Authors and Affiliations

  1. Limnological Institute, Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia

    A. V. Lomakina, E. V. Mamaeva, T. V. Pogodaeva, I. A. Khal’zov & T. I. Zemskaya

  2. Vinogradov Institute of Geochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia

    G. V. Kalmychkov

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  1. A. V. Lomakina
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  2. E. V. Mamaeva
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  3. T. V. Pogodaeva
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  4. G. V. Kalmychkov
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  5. I. A. Khal’zov
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  6. T. I. Zemskaya
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Correspondence to A. V. Lomakina.

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Original Russian Text © A.V. Lomakina, E.V. Mamaeva, T.V. Pogodaeva, G.V. Kalmychkov, I.A. Khal’zov, T.I. Zemskaya, 2018, published in Mikrobiologiya, 2018, Vol. 87, No. 3.

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Lomakina, A.V., Mamaeva, E.V., Pogodaeva, T.V. et al. Anaerobic Methane Oxidation in Enrichment Cultures from Deep Sediments of a Mud Volcano Peschanka (South Baikal). Microbiology 87, 317–325 (2018). https://doi.org/10.1134/S0026261718030049

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  • DOI: https://doi.org/10.1134/S0026261718030049

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