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Anaerobic cellulolytic microbial communities decomposing the biomass of Anabaena variabilis

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Abstract

Four previously isolated methanogenic anaerobic consortia, which were originally cultivated on a cellulose-containing substrate (filter paper), were used as inocula for the anaerobic conversion of the biomass of Anabaena variabilis into biogas at 55°C. The cumulative methane yield in the biogas produced by the most active consortia reached 64%. However, the biotransformation was only efficient in the course of the single inoculation and pretreatment of the cyanobacterial biomass by its concentration and freeze-thawing. The DGGE analysis of the structure of the selected microbial consortia, cultivated on the filter paper, revealed qualitative variations in the biodiversity of predominant Bacteria, showing differences in band number and intensity. The composition of methanogenic Archaea in these consortia was similar, with the presence of the genera Methanoculleus and Methanosarcina. The efficiency of the microbial consortia selection, and the role of the various microbial trophic groups in bioconversion of the substrates, such as cellulose and the biomass of phototrophic microorganisms are discussed.

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Authors and Affiliations

  1. Department of Microbiology, Biological Faculty, Lomonosov Moscow State University, Moscow, Russia

    E. V. Petrova, M. A. Egorova, N. F. Piskunkova, A. I. Netrusov & E. A. Tsavkelova

  2. Department of Soil Biology, Faculty of soil Sciences, Lomonosov Moscow State University, Moscow, Russia

    P. A. Kozhevin

Authors
  1. E. V. Petrova
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  2. M. A. Egorova
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  3. N. F. Piskunkova
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  4. P. A. Kozhevin
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  5. A. I. Netrusov
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  6. E. A. Tsavkelova
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Correspondence to E. A. Tsavkelova.

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Original Russian Text © E.V. Petrova, M.A. Egorova, N.F. Piskunkova, P.A. Kozhevin, A.I. Netrusov, E.A. Tsavkelova, 2017, published in Mikrobiologiya, 2017, Vol. 86, No. 6, pp. 729–738.

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Petrova, E.V., Egorova, M.A., Piskunkova, N.F. et al. Anaerobic cellulolytic microbial communities decomposing the biomass of Anabaena variabilis. Microbiology 86, 745–752 (2017). https://doi.org/10.1134/S0026261717060133

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