Skip to main content

Biodiversity of aerobic methylobacteria associated with the phyllosphere of the southern Moscow region


During the summer period (15–25°C), 34 strains of methylotrophic bacteria associated with different species of herbs, shrub, and trees in Pushchino (Moscow oblast, Russia) were isolated on the medium with methanol. Predominance of pink-colored Methylobacterium strains in the phyllosphere of many plants was confirmed by microscopy, enumeration of the colonies from grass leaves, and sequencing of the 16S rRNA genes. Colorless and yellow-pigmented methylotrophs belonged to the genera Methylophilus, Methylobacillus, Hansschlegelia, Methylopila, Xanthobacter, and Paracoccus. All isolates were able to synthesize plant hormones auxins from L-tryptophan (5−50 μg/mL) and are probably plant symbionts.

This is a preview of subscription content, access via your institution.


  • Agafonova, N.V., Kaparullina, E.N., Doronina, N.V., and Trotsenko, Yu.A., Phosphate-solubilizing activity of aerobic methylobacteria, Microbiology (Moscow), 2013, vol. 82, pp. 864–867.

    Article  CAS  Google Scholar 

  • Doronina, N.V., Ivanova, E.G., Suzina, N.E., and Trotsenko, Yu.A., Methanotrophs and methylobacteria are found in woody plant tissues within the winter period, Microbiology (Moscow), 2004, vol. 73, no. 6, pp. 702–709.

    Article  CAS  Google Scholar 

  • Doronina, N.V., Torgonskaya, M.L., Fedorov, D.N., and Trotsenko, Y.A., Aerobic methylobacteria as promising objects of modern biotechnology (Review), Appl. Biochem. Microbiol., 2015, vol. 51, no. 2, pp. 125–134.

    Article  CAS  Google Scholar 

  • Dourado, M.N., Neves, A.A.C., Santos, D.S., and Araujo, W.L., Biotechnological and agronomic potential of endophytic pink-pigmented methylotrophic Methylobacterium spp., Bio. Med. Res. Int., 2015, vol. 2015, pp. 1–19.

    Google Scholar 

  • Fedorov, D.N., Doronina, N.V., and Trotsenko, Y.A., Phytosymbiosis of aerobic methylobacteria: New facts and views, Microbiology (Moscow), 2011, vol. 80, no. 4, pp. 443–454.

    Article  CAS  Google Scholar 

  • Fedorov, D.N., Ivanova, E.G., Doronina, N.V., and Trotsenko, Yu.A., A new system of degenerate oligonucleotide primers for detection and amplification of nifHD genes, Microbiology (Moscow), 2008, vol. 77, no. 2, pp. 247–249.

    Article  CAS  Google Scholar 

  • Gogleva, A.A., Kaparullina, E.N., Doronina, N.V., and Trotsenko, Y.A., Methylophilus flavus sp. nov.,and Methylophilus luteus sp. nov., aerobic, methylotrophic bacteria associated with plants, Int. J. Syst. Evol. Microbiol., 2010, vol. 60, pp. 2623–2628.

    Article  CAS  PubMed  Google Scholar 

  • Gordon, S.A. and Weber, R.P., Colorimetric estimation of indoleacetic acid, Plant Physiol., 1951, vol. 26, pp. 192–195.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Holland, M.A., Methylobacterium and plants, Recent Res. Develop. Plant Physiol., 1997, vol. 1, pp. 207–213.

    Google Scholar 

  • Ivanova, E., Doronina, N., and Trotsenko, Yu., Hansschlegelia plantiphila gen. nov.,sp. nov., a new aerobic restricted facultative methylotrophic bacterium associated with plants, Syst. Appl. Microbiol., 2007, vol. 30, pp. 444–452.

    Article  CAS  PubMed  Google Scholar 

  • Ivanova, E.G., Doronina, N.V., Laman, A.G., Brovko, F.A., Trotsenko, Yu.A., and Shepelyakovskaya, A.O., Facultative and obligate aerobic methylobacteria synthesize cytokinins, Microbiology (Moscow), 2000, vol. 69, no. 6, pp. 646–651.

    Article  CAS  Google Scholar 

  • Ivanova, E.G., Fedorov, D.N., Doronina, N.V., and Trotsenko, Yu.A., Production of vitamin B12 in aerobic methylotrophic bacteria, Microbiology (Moscow), 2006, vol. 75, no. 4, pp. 494–496.

    Article  CAS  Google Scholar 

  • Jourand, P., Giraud, E., Bena, G., Sy, A., Willems, A., Gillis, M., Dreyfus, B., and De Lajudie, P., Methylobacterium nodulans sp. nov., for a group of aerobic, facultatively methylotrophic, legume root-nodule forming and nitrogen-fixing bacteria, Int. J. Syst. Evol. Microbiol., 2004, vol. 54, pp. 2269–2273.

    CAS  PubMed  Google Scholar 

  • Kim, M., Oh, H.S., Park, S.C., and Chun, J., Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes, Int. J. Syst. Evol. Microbiol., 2014, vol. 64, pp. 346–351.

    Article  CAS  PubMed  Google Scholar 

  • Lacava, P.T., Silva-Stenico, M.E., Araújo, W.L., Simionato, A.V.C., Carrilho, E.L., Tsai, S.M., and Azevedo, J.L., Detection of siderophores in endophytic bacteria Methylobacterium spp. associated with Xylella fastidiosa subsp. pauca, Pesq. Agropec. Bras., 2008, vol. 43, no. 4, pp. 521–528.

    Article  Google Scholar 

  • Romanovskaya, V.A., Stolyar, S.M., Malashenko, Y.R., and Dodatko T.N., The ways of plant colonization by Methylobacterium strains and properties of these bacteria, Microbiology (Moscow), 2001, vol. 70, pp. 221–227. doi 10.1023/A:1010441900060

    Article  CAS  Google Scholar 

  • Sanders, J.W., Martin, J.W., Hooke, M., and Hooke, J. Methylobacterium mesophilicum infection: case report and literature review of an unusual opportunistic pathogen, Clin. Infect. Dis., 2000, vol. 30, pp. 936–938.

    Article  CAS  PubMed  Google Scholar 

  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol., 2011, vol. 28, pp. 2731–2739.

    Article  CAS  PubMed  Google Scholar 

  • Tani, A., Sahin, N., Fujitani, Y., Kato, A., Sato, K., and Kimbara, K., Methylobacterium species promoting rice and barley growth and interaction specificity revealed with whole-cell matrix-assisted laser desorption/ionizationtime- of-flight mass spectrometry (MALDI-TOF/MS) analysis, PLoS One, 2015. 10:e0129509. doi 10.1371/journal.pone.0129509

    Article  PubMed  PubMed Central  Google Scholar 

  • Trotsenko, Yu.A., Doronina, N.V., and Torgonskaya, M.L., Aerobnye metilobakterii (Aerobic Methylobacteria), Gal’chenko, V.F., Ed., Pushchino: ONTI PNTs RAN, 2010.

  • Van de Peer, Y. and De Wachter, R., TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment, Comput. Appl. Biosci., 1994, vol. 10, pp. 569–570.

    PubMed  Google Scholar 

  • Vorholt, J.A., Microbial life in the phyllosphere, Nat. Rev. Microbiol., 2012, vol. 10, pp. 828–840.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Yu. A. Trotsenko.

Additional information

Original Russian Text © E.N. Kaparullina, N.V. Doronina, I.I. Mustakhimov, N.V. Agafonova, Yu.A. Trotsenko, 2017, published in Mikrobiologiya, 2017, Vol. 86, No. 1, pp. 107–113.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kaparullina, E.N., Doronina, N.V., Mustakhimov, I.I. et al. Biodiversity of aerobic methylobacteria associated with the phyllosphere of the southern Moscow region. Microbiology 86, 113–118 (2017).

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI:


  • methylobacteria
  • biodiversity
  • phyllosphere
  • herbs
  • shrubs
  • and trees
  • auxins