Abstract
Present study was carried out to evaluate a new bacterial strain, Lactobacillus delbreuckii subsp. bulgaricus F18 as probiotic strain. L. delbreuckii subsp. bulgaricus F18 was isolated from homemade butter and identified by conventional and molecular techniques. The 16S rRNA sequence of the isolate was registered in National Centre for Biotechnology Information (NCBI) under accession number KT865224. In the present study, L. delbreuckii subsp. bulgaricus F18 exhibited highest viable counts against acid tolerance or low pH tolerance (at pH 1 after 2h of incubation), bile tolerance (conc. 1%), autoaggregation (68%), cell surface hydrophobicity against O-xylene (33.9%), antimicrobial activity against various food borne pathogens (inhibition = 100%), antibiotic sensitivity following standard test methods suggested by various research workers.
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Begley, E., Grzeskowiak, B., Mundigler, N., Sentall, W, and Walcak, J., Antioxidative properties of faba bean-,soybean- and oat tempeh, Ind. J. Food. Sci. Nutr., 2005, vol. 49, pp. 45–54.
Borgstrom, G., Principals of Food Science. Food Microbiology and Biochemistry, New York: Macmillan, 1968.
Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI, 2011.
Collado, M., Meriluoto, J., and Salminen, S., In vitro analysis of probiotic strain combinations to inhibit pathogen adhesion to human intestinal mucus, Food Res. Internat., 2007, vol. 40, pp. 629–639.
Delgado, O., The soya route in northwest Argentina, in The United Soy Republics?, Rulli, J., Ed. (www.lasojamata.org), 2007.
Guidelines for the Evaluation for the Probiotics in Food: Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food, FAO/WHO, 2002.
Gautam, N., and Sharma, N., Evaluation of probiotic potential of new bacterial strain, Lactobacillus spicheri G2 isolated from Gundruk, Proc. Natl. Acad. Sci., India., Sect. B: Biol. Sci, 2015, vol. 85, pp. 979–986.
Gotcheva, V., Hristozova, E., Hristozova, T., Guo, M., Roshkova, Z, and Angelov, A., Assessment of potential probiotic properties of lactic acid bacteria and yeast strains, Food Biotechnol., 2002, vol. 16, pp. 211–225.
Hati, S., Vij, S., Mandal, S., Yadav, D., and Vandna A., of L. plantarum C6 isolated from cheese, Ind. J. Dairy Sci., 2014, vol. 67, no. 5, pp. 413–420.
Havenaar, R., and Huis in’t Veld, J.H.J., Probiotics: a general view, in The Lactic Acid Bacteria in Health and Disease, Wood, B.J.B., Ed., London: Elsevier Appl. Sci., London, 1992.
Jin, H., Hartman, G.L., Nickel, D., and Widholm, J.M., Phytotoxicity of culture filtrate of Fusarium solani, the causal agent of sudden death syndrome of soybean, Plant Dis., 1996, vol. 80, pp. 922–927.
Kimura, H., Sashihara, T., Matsusaki, H., Sonomoto, K., and Ishizaki, A., Novel bacteriocin of Pediococcus sp. ISK-1 isolated from well-aged bed of fermented rice bran, Ann. New York Acad. Sci., 1998, vol. 864, pp. 345–348.
Kos, B., Suškovic, J., Vukovic, S., Simpraga, M., Frece, J., and Matošic, S., Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92, J. Appl. Microbiol., 2003, vol. 94, pp. 981–987.
Kurmann, J.A. and Rasic, J.L., The health potential of products containing bifidobacteria, in Therapeutic Properties of Fermented Milks, Robinson, R.K., Ed., London: Elsevier Appl. Food Sci., 1991, pp. 117–158.
Licht, T.R. and Wilcks, A., Conjugative gene transfer in the gastrointestinal environment, Adv. Appl. Microbiol., 2005, vol. 58, pp. 77–95.
Lindgren, S.E. and Dorbogosz, W.J., Antagonistic activities of lactic acid bacteria in food and feed fermentation, FEMS, Microbiol. Rev., 1990, vol. 87, pp. 149–163.
Martin, R., Jimenez, E., Olivares, M., Marin, M.L., Fernanderz, L., Xaus, J., and Rodriguez, J.M., Lactobacillus salivarius CECT 5713,a potential probiotic strain isolated from infant feces and breast milk of a mother-child pair, Int. J. Food. Microbiol., 2006, vol. 122, pp. 35–43.
Musikasang, H., Tani, A., Kittikun, A., and Maneerat, S., Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract, World. J. Microbiol. Biotechnol., 2009, vol. 25, pp. 1337–1345.
Orlowski, A. and Bielecka, M., Preliminary characteristics of Lactobacillus and Bifidobacterium strains as probiotic candidates, Polish J. Food. Nutr. Sci., 2006, vol. 56, no. 3, pp. 269–275.
Parvez, S., Malik, K.A., Kang, A.H., and Kim, H.Y., Probiotics and their fermented food products are beneficial for health, J. Appl.Microbiol., 2006, vol. 100, pp. 1171–1185.
Rosenberg, M., Gutnick, D., and Rosenberg, E., Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity, FEMS Microbiol. Lett., 1980, vol. 9, pp. 29–33.
Rybka, S. and Fleet, G.H., Population of Lactobacillus delbrueckii ssp. bulgaricus,Streptococcus thermophilus,Lactobacillus acidophilus and Bifidobacterium species in Australian yoghurts, Food Aus., 1997, vol. 49, pp. 471–475.
Wicklund, T. and Narvhus, J.A., Growth and metabolism of selected strains of probiotic bacteria: in maize porridge with added malted barley, I. J. Food Microbiol., 2004, vol. 91, pp. 305–313.
Xanthopoulos, V., Litopoulou-Tzanetaki, E., and Tzanetakis, N., In vitro study of Lactobacillus species strains on bile tolerance and cholesterol removal, in Lactic Acid Bacteria–Lactic 97, Caen: Presses universitaires de Caen, 1997.
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Handa, S., Sharma, N. In vitro probiotic potential of Lactobacillus delbreuckii subsp. bulgaricus F18 isolated from homemade butter. Microbiology 86, 143–149 (2017). https://doi.org/10.1134/S0026261716060114
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DOI: https://doi.org/10.1134/S0026261716060114