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Biofilm of Aureobasidium pullulans var. pullulans on winter wheat kernels and its effect on other microorganisms

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Abstract

Winter wheat, grown under greenhouse conditions, was protected four times with a cell suspension of Aureobasidium pullulans var. pullulans during the growing season. After harvest, the distribution and survival rates of the studied biocontrol agent were analyzed under a scanning electron microscope. The abundance of filamentous fungi, yeasts, pseudomonads and Azotobacter bacteria was determined by inoculation onto selective agar media. A. pullulans produced mostly unicellular chlamydospores on the surface and in the brush of kernels. Multicellular blastospore conglomerates secreted extracellular polymeric substances (EPS), and their biofilms were found in the brush and crease of kernels. The application of a cell suspension of A. pullulans with the density of 104 CFU to winter wheat spikes, repeated four times, inhibited the growth of pseudomonads, Azotobacter bacteria and filamentous fungi.

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References

  • Cordier, J.L. and Gram, L., Cereals and Cereal Products, in Microorganisms in Foods. Microbial Ecology of Food Commodities, Roberts, T.A., Cordier, J.-L., Gram, L., Tompkin, R.B., Pitt, J.I., Gorris, L.G.M., and Swanson, K.M.J, Eds., Springer, 2005, pp. 392–439.

    Google Scholar 

  • Danhorn, T. and Fuqua, C., Biofilm formation by plantassociated bacteria, Annu. Rev. Microbiol., 2007, vol. 61, pp. 401–422.

    Article  CAS  PubMed  Google Scholar 

  • Donot, F., Fontana A., Baccou, J.C., and Schorr-Galindo, S., Microbial exopolysaccharides: main examples of synthesis, excretion, genetics and extraction, Carbohyd. Polym., 2012, vol. 87, pp. 952–962.

    Article  Google Scholar 

  • Fleet, G.H., Yeasts in foods and beverages: impact on product quality and safety, Curr. Opin. Biotech., 2007, vol. 18, pp. 170–175.

    Article  CAS  PubMed  Google Scholar 

  • Gaur, R., Singh, R., Gupta, M., Kumar, M., and Gaur, M.K., Aureobasidium pullulans, an economically important polymorphic yeast with special reference to pullulan, Afr. J. Biotechnol., 2010, vol. 9, pp. 7989–7997.

    Article  CAS  Google Scholar 

  • Gniewosz, M., and Duszkiewicz-Reinhard, W., Comparative studies on pullulan synthesis, melanin synthesis and morphology of white mutant Aureobasidium pullulans B-1 and parent strain A.p.-3, Carbohyd. Polym., 2008, vol. 72, pp. 431–438.

    Article  CAS  Google Scholar 

  • Harding, M.W., Marques, L.L., Howard, R. J., and Olson, M.E., Can filamentous fungi form biofilms? Trends Microbiol., 2009, vol. 17, pp. 475–480.

    Article  CAS  PubMed  Google Scholar 

  • Ippolito, A., El Ghaouth, A., Wilson, C.L., and Wisniewski, M. Control of postharvest decay of apple fruit by Aureobasidium pullulans and induction of defense responses, Postharvest Biol. Tec., 2000, vol. 19, pp. 265–272.

    Article  CAS  Google Scholar 

  • Irzykowski, W., Soldatova, V., Gasich, E., Razgulaeva, N. and Jedryczka, M., RAPD analysis of Sclerotinia sclerotiorum from crucifers, IOBC-WPRS Bull. 2005, vol. 28, pp. 69–82.

    Google Scholar 

  • Ito, M., Sato, I., Ishizaka, M., Yoshida, S., Koitabashi, M., Yoshida, S., and Tsushima, S., A bacterial cytochrome P450 system catabolizing the Fusarium toxin deoxynivalenol, Appl. Environ. Microbiol., 2013, vol. 79, pp. 1619–1628.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jackowiak, H., Packa, D., Wiwart, M., and Perkowski, J., Scanning electron microscopy of Fusarium damaged kernels of spring wheat, Int. J. Food Microbiol., 2005, vol. 98, pp. 113–123.

    Article  CAS  PubMed  Google Scholar 

  • Kachalkin, A. V., New Data on the distribution of certain psychrophilic yeasts in Moscow oblast. Microbiology (Moscow), 2010, vol. 79, no. 6, pp. 840–844.

    Article  CAS  Google Scholar 

  • King, E.O., Ward, M.K., and Raney, D.E., Two simple media for the demonstration of pyocyanin and fluorescein, J. Lab. Clin. Med., 1954, vol. 44, pp. 301–307.

    CAS  PubMed  Google Scholar 

  • Kocková-Kratochvílová, A., Cernáková, M., and Sláviková, E., Morphological changes during the life cycle of Aureobasidium pullulans (de Bary) Arnaud, Folia Microbiol., 1980, vol. 25, pp. 56–67.

    Article  Google Scholar 

  • Laca, A., Mousia, Z., Diaz, M., Webb, C., and Pandiella S.S., Distribution of microbial contamination within cereal grains, J. Food Eng., 2006, vol. 72, pp. 332–338.

    Article  Google Scholar 

  • Marquina, D., Santos, A., and Peinado, J.M., Biology of killer yeasts, Int. Microbiol., 2002. vol. 5, pp. 65–71.

    Article  CAS  PubMed  Google Scholar 

  • Martin, J.P., Use of acid, rose bengal and streptomycyn in the plate method for estimating soil fungi, Soil Sci., 1950, vol. 38, pp. 215–232.

    Article  Google Scholar 

  • Martyniuk, S. and Martyniuk, M., Occurence of Azotobacter spp. in Polish soils, Pol. J. Environ. Stud., 2003, vol. 12, pp. 371–374.

    CAS  Google Scholar 

  • Metzger, U., Lankes, U., Hardy, E.H., Gordalla, B.C., and Frimmel, F.H., Monitoring the formation of an Aureobasidium pullulans biofilm in a bead-packed reactor via flowweighted magnetic resonance imaging, Biotechnol. Lett., 2006, vol. 28. pp. 1305–1311.

    Article  CAS  PubMed  Google Scholar 

  • Olstrope, M., Borling, J., Schnurer, J., and Passoth, V., Pichia anomala yeast improves feed hygiene during storage of moist crimped barley grain under swedish farm conditions, Anim. Feed Sci. Tech., 2010, vol. 156, pp. 37–46.

    Article  Google Scholar 

  • Polish National List of Agricultural Plant Varieties, Research Centre for Cultivar Testing (COBORU), Slupia Wielka, 2012.

  • Prasongsuk, S., Sullivan, R.F., Kuhirun, M., Eveleigh, D.E., and Punnapayak, H., Thailand habitats as sources of pullulan-producing strains of Aureobasidium pullulans, World J. Microb. Biotechnol., 2005, vol. 21, pp. 393–398.

    Article  CAS  Google Scholar 

  • Raacke, I.C., von Rad, U., Mueller, M.J., and Berger, S., Yeast increases resistance in arabidopsis against Pseudomonas syringae and Botrytis cinerea by salicylic acid-depemdent as well as-independent mechanisms, Mol. Plant-Microbe Interact., 2006, vol. 19, pp. 1138–1146.

    Article  CAS  PubMed  Google Scholar 

  • Raulio, M., Wilhelmson, A., Salkinoja-Salonen, M., and Laitila, A., Ultrastructure of biofilms formed on barley kernels during malting with and without starter culture, Food Microbiol., 2009, vol. 26, pp. 437–443.

    Article  CAS  PubMed  Google Scholar 

  • Sailer, M.F. and Knol, W., Forming of a functional biofilm on wood surfaces. Ecol. Eng., 2010, vol. 36, pp. 163–167.

    Article  Google Scholar 

  • Schena, L., Nigro, F., Pentimone, I., Ligorio, A., and Ippolito, A., Control of postharvest rots of sweet cherries and table grapes with endophytic isolates of Aureobasidium pullulans, Postharvest Biol. Technol., 2003, vol. 30, pp. 209–220.

    Article  Google Scholar 

  • Schisler, D.A., Slininger, P.J., Boehm, M.J., and Paul, P.A., Co-culture of yeast antagonists of fusarium head blight and their effect on disease development in wheat, Plant Pathol. J., 2011, vol. 10, pp. 128–137.

    Article  Google Scholar 

  • Srivastava, R.B., Awasthi, M., Upreti, M.C., and Mathur, G.N., Studies on Aureobasidium pullulans forming biofilm on high strength aluminum alloy, a structural component, in aircraft fuel tanks, Indian J. Eng. Mater. Sci., 2006, vol. 13, pp. 135–139.

    CAS  Google Scholar 

  • STATISTICA (data analysis software system), version 10, StatSoft, Inc. 2011. www.statsoft.com.

  • Vero, S., Mondino, P., Burgueño, J., Soubes, M., and Wisniewski, M., Characterization of biocontrol activity of two yeast strains from uruguay against blue mold of apple, Postharvest Biol. Tec., 2002, vol. 26, pp. 91–98.

    Article  Google Scholar 

  • Wachowska, U., Kucharska, K., Jedryczka, M., and Lobik, N., Microorganisms as biological control agents against Fusarium pathogens in winter wheat, Pol. J. Environ. Stud., 2013, vol. 22, pp. 591–597.

    Google Scholar 

  • West, T.P., Effect of carbon source on polysaccharide production by alginate-entrapped Aureobasidium pullulans ATCC 42023 cells, J. Basic Microbiol., 2011, vol. 51, pp. 673–677.

    Article  CAS  PubMed  Google Scholar 

  • White, T.J., Bruns, T., Lee, S., and Taylor, J.W., Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, in PCR Protocols: A Guide to Methods and Applications, Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J., Eds., New York: Academic, 1990, pp. 315–322.

    Google Scholar 

  • Zalar, P., Gostinčar, C., de Hoog, G.S., Uršič, V., Sudhadham, M., and Gunde-Cimerman, N., Redefinition of Aureobasidium pullulans and its varieties, Stud. Mycol., 2008, vol. 61, pp. 21–38.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Entomology, Phytopathology and Molecular Diagnostic, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-720, Olsztyn, Poland

    U. Wachowska, W. Mikołajczyk & K. Kucharska

  2. Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-720, Olsztyn, Poland

    K. Głowacka

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  1. U. Wachowska
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  2. K. Głowacka
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  3. W. Mikołajczyk
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  4. K. Kucharska
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Correspondence to U. Wachowska.

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Wachowska, U., Głowacka, K., Mikołajczyk, W. et al. Biofilm of Aureobasidium pullulans var. pullulans on winter wheat kernels and its effect on other microorganisms. Microbiology 85, 523–530 (2016). https://doi.org/10.1134/S0026261716050192

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