Abstract
Biosurfactans are amphiphilic compounds synthesized by a wide group of microorganisms and tend to interact with surfaces of different polarities. In the present study we purified and characterized a biosurfactant produced by Dietzia cinnamea KA-1 when cultured by n-hexadecane as sole carbon source. The crude biosurfactant was extracted with ethyl acetate and purified by freezing at–20°C and then silica Gel column chromatography. The purified biosurfactant applied for more characterization using Elemental analysis (CHNS), Fourier Transform Infrared Spectroscopy (FTIR), Mass Spectroscopy (MS) and Nuclear Magnetic Resonance (1H and 13C-NMR) analysis. CHNS analysis showed the presence of C (74.92%) and H (11.63%) but not N or S. Functional groups of OH, CH2, CH3, C=O and aliphatic C−O revealed by FTIR analysis. The presence and position of these groups were confirmed by NMR analysis, and molecular mass of biosurfactant calculated using MS analysis. Finally, the product characterized as a methylated ester compound with molecular formula of C21H42O4. This is the first report of biosurfactant of species D. cinnamea identified as ester, furthermore the ester was found to be in the methylated form.
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Bharali, P. and Konwar, B.K., Production and physicochemical characterization of a biosurfactant produced by Pseudomonas aeruginosa OBP1 isolated from petroleum sludge, Appl. Biochem. Biotechnol., 2011, vol. 164, pp. 1444–1460.
Coates, J., Interpretation of infrared spectra, a practical approach, in Encyclopedia of Analytical Chemistry, 1st ed., Meyers, R.A., Ed., Chichester, 2000, pp. 10815–10837.
Dewick, P.M., Medicinal Natural Products: A Biosynthetic Approach, 3rd ed., Wiley, 2009, pp. 149–150.
Ebrahimipour, G.H., Gilavand, F., Karkhane, M., Kavyanifard, A.A., Teymouri, M., and Marzban, A., Bioemulsification activity assessment of an indigenous strain of halotolerant Planococcus and partial characterization of produced biosurfactants, Int. J. Environ. Sci. Technol., 2014. doi 10.1007/s13762-014-0548-5
Geys, R., Soetaert, W., and Bogaert, I.V., Biotechnological opportunities in biosurfactant production, Curr. Opin. Biotechnol., 2014, vol. 30, pp. 66–72. doi 10.1016/j.copbio. 2014.06.002
Kavynifard, A., Ebrahimipour, Gh., and Ghassempour, A., Individually and Synergistic Degradation of Hydrocarbons by Biosurfactant Producing Bacteria, Res Mol Med., 2016, Vol. 4 (1), pp. 36–44. doi 10.7508/rmm.2016.01.006
Kavynifard, A., Ebrahimipour, G., and Ghasempour, A., Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production, J. Basic Microbiol., 2015, vol. 55. doi 10.1002/jobm.201500386
Liu, J., Huang, X.F., Lu, L.J., Xu, J.C., Wen, Y., Yang, D.H., and Zhou, Q., Comparison between waste frying oil and paraffin as carbon source in the production of biodemulsifier by Dietzia sp. S-JS-1, Bioresour. Technol., 2009, vol. 100, pp. 6481–6487.
Lu, C., Shu-Qin, L., and Yan-Fen, X., Characterization of an alkaliphilic biosurfactant producing strain of Dietzia, Microbiol. China, 2012, vol. 39, pp. 1573–1579. http://journals. im.ac.cn/wswxtbcn
Nakano, M., Kihara, M., Iehata, S., Tanaka, R., Maeda, H., and Yoshikawa, T., Wax ester-like compounds as biosurfactants produced by Dietzia maris from n-alkane as a sole carbon source, J. Basic. Microbiol., 2011, vol. 51, pp. 490–498.
Satpute, S.K., Banpurkar, A.G., Dhakephalkar, P.K., Banat, I.M., and Chopade, B.A., Methods for investigating biosurfactants and bioemulsifiers: a review, Crit. Rev. Biotechnol., 2010, pp. 127–144.
Schlegel, H.G., Allgemeine Mikrobiologie, 7th ed., Stuttgart: Georg Thieme, 1992.
Shaligram, N.S. and Singhal, R.S., Surfactin—a review on biosynthesis, fermentation, purification and applications, Food Technol. Biotechnol., 2010, vol. 48, pp. 119–134.
Smyth, T.J.P., Perfumo, A., Marchant, R., and Banat, I.M., Isolation and analysis of low molecular weight microbial glycolipids in Handbook of Hydrocarbon and Lipid Microbiology, 2010, pp. 3705–3723.
Sutcliffe, I.C., Characterisation of a lipomannan lipoglycan from the mycolic acid containing actinomycete Dietzia maris, Antonie van Leeuwenhoek., 2000 vol. 78, pp. 195–201. doi 10.1023/A:1026562610490
Wang, W., Cai, B., and Shao, Z., Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3, Frontiers Microbiol., 2014, vol. 5, p. 711. doi 10.3389/fmicb.2014.00711
Wang, X.B., Nie, Y., Tang, Y.Q., Wu, G., and Wu, X.L., n-Alkane chain length alters Dietzia sp. strain DQ12-45-1b biosurfactant production and cell surface activity, Appl. Environ. Microbiol., 2013, vol. 79, pp. 400–402. doi 10.1128/AEM.02497-12
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Kavyanifard, A., Ebrahimipour, G. & Ghasempour, A. Structure characterization of a methylated ester biosurfactant produced by a newly isolated Dietzia cinnamea KA1. Microbiology 85, 430–435 (2016). https://doi.org/10.1134/S0026261716040111
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DOI: https://doi.org/10.1134/S0026261716040111