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Hydrocarbon biodegradation and surfactant production by acidophilic mycobacteria

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Abstract

Production of biosurfactants by acidophilic mycobacteria was demonstrated in the course of aerobic degradation of hydrocarbons (n-tridecane, n-tricosane, n-hexacosane, model mixtures of С14–С17, С12‒С19, and С9–С21 n-alkanes, 2,2,4,4,6,8,8-heptamethylnonane, squalane, and butylcyclohexane) and their complex mixtures (hydrocarbon gas condensate, kerosene, black oil, and paraffin oil) under extremely acidic conditions (pH 2.5). When grown on hydrocarbons, the studied bacterial culture AGS10 caused a decrease in the surface and interfacial tension of the solutions (to the lowest observed values of 26.0 and 1.3 mN/m, respectively) compared to the bacteria-free control. The rheological characteristics of the culture changed only when mycobacteria were grown on hydrocarbons. Neither the medium nor the cell-free culture liquid had the surfactant activity, which indicated formation of an endotype biosurfactant by mycobacteria. Biodegradation of n-alkanes was accompanied by an increase in cell numbers, surfactant production, and changes in the hydrophobicity of bacterial cell surface and in associated phenomena of adsorption and desorption to the hydrocarbon phase. Research on AGS10 culture liquids containing the raw biosurfactant demonstrated the preservation of its activity within a broad range of pH, temperature, and salinity.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia

    A. E. Ivanova & D. Sh. Sokolova

  2. Topchiev Institute of Petrochemical Synthesis, Moscow, Russia

    A. Yu. Kanat’eva

Authors
  1. A. E. Ivanova
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  2. D. Sh. Sokolova
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  3. A. Yu. Kanat’eva
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Correspondence to A. E. Ivanova.

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Original Russian Text © A.E. Ivanova, D.Sh. Sokolova, A.Yu. Kanat’eva, 2016, published in Mikrobiologiya, 2016, Vol. 85, No. 3, pp. 300–308.

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Ivanova, A.E., Sokolova, D.S. & Kanat’eva, A.Y. Hydrocarbon biodegradation and surfactant production by acidophilic mycobacteria. Microbiology 85, 317–324 (2016). https://doi.org/10.1134/S002626171603005X

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