Abstract
The previously obtained insertion mutants of Azospirillum brasilense Sp245 in the genes mmsB1 and fabG1 (strains SK039 and Sp245.1610, respectively) were characterized by impaired flagellation and motility. The putative products of expression of these genes are 3-hydroxyisobutyrate dehydrogenase and 3-oxoacyl-[acyl-carrier protein] reductase, respectively. In the present work, A. brasilense strains Sp245, SK039, and Sp245.1610 were found to have differences in the content of 3-hydroxyhexadecanoic, hexadecanoic, 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, and nonadecanoic acids in their lipopolysaccharide preparations, as well as in cell hydrophobicity and hemagglutination activity and dynamics of cell aggregation, in biomass amount, and in the relative content of lipopolysaccharide antigens in mature biofilms formed on hydrophilic or hydrophobic surfaces.
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References
Baldani, V.L.D., Baldani, J.I., and Döbereiner, J., Effects of Azospirillum inoculation on root infection and nitrogen incorporation in wheat, Can. J. Microbiol., 1983, vol. 29, no. 8, pp. 924–929.
Bogino, P.C., Oliva, M.M., Sorroche, F.G., and Giordano, W., The role of bacterial biofilms and surface components in plant-bacterial associations, Int. J. Mol. Sci., 2013, vol. 14, pp. 15838–15859.
Chowdhury, E.K., Nagata, S., and Misono, H., 3-Hydroxyisobutyrate dehydrogenase from Pseudomonas putida E23: purification and characterization, Biosci. Biotechnol. Biochem., 1996, vol. 60, no. 12, pp. 2043–2047.
Croes, C.L., Moens, S., van Bastelaere, E., Vanderleyden, J., and Michiels K.W., The polar flagellum mediates Azospirillum brasilense adsorption to wheat roots, J. Gen. Microbiol., 1993, vol. 139, no. 9, pp. 2261–2269.
Döbereiner, J. and Day, J.M., Associative symbiosis in tropical grass: characterization of microorganisms and dinitrogen fixing sites, in Symposium on Nitrogen Fixation, Newton, W.E. and Nijmans, C.J., Eds., Pullman: Washington State Univ. Press, 1976, pp. 518–538.
Fedonenko, Yu.P., Zdorovenko, E.L., Konnova, S.A., Ignatov, V.V., and Shlyakhtin, G.V., A comparison of the lipopolysaccharides and O-specific polysaccharides of Azospirillum brasilense Sp245 and its Omegon-Km mutants KM018 and KM252, Microbiology (Moscow), 2004, vol. 73, no. 2, pp. 143–149.
Flemming, H.-C. and Wingender, J., The biofilm matrix, Nat. Rev. Microbiol., 2010, vol. 8, no. 9, pp. 623–633.
Guttenplan, S.B. and Kearns, D.B., Regulation of flagellar motility during biofilm formation, FEMS Microbiol. Rev., 2013, vol. 37, no. 6, pp. 849–871.
Hall, P.G. and Krieg, N.R., Swarming of Azospirillum brasilense on solid media, Can. J. Microbiol., 1983, vol. 29, no. 11, pp. 1592–1594.
Kovtunov, E.A., Shelud’ko, A.V., Chernyshova, M.P., Petrova, L.P., and Katsy, E.I., Mutants of bacterium Azospirillum brasilense Sp245 with Omegon insertion in mmsB or fabG genes of lipid metabolism are defective in motility and flagellation, Russ. J. Genet., 2013, vol. 49, no. 11, pp. 1107–1111.
Lai, H.-C., Soo, P.-C., Wei, J.-R., Yi, W.-C., Liaw, S.-J., Horng, Y.-T., Lin, S.-M., Ho, S.-W., Swift, S., and Williams, P., The RssAB two-component signal transduction system in Serratia marcescens regulates swarming motility and cell envelope architecture in response to exogenous saturated fatty acids, J. Bacteriol., 2005, vol. 187, no. 10, pp. 3407–3414.
Lindahl, A., Faris, A., Wadström, T., and Hjertén S., A new test based on “salting out” to measure relative surface hydrophobicity of bacterial cells, Biochim. Biophys. Acta, 1981, vol. 677, nos. 3/4, pp. 471–476.
López, D., Vlamakis, H., and Kolter, R., Biofilms, Cold Spring Harb. Perspect. Biol., 2010, vol. 2, no. 7, p. a000398.
Madi, L. and Henis, Y., Aggregation in Azospirillum brasilense Cd: conditions and factors involved in cell–to cell adhesion, Plant Soil, 1989, vol. 115, no. 1, pp. 89–98.
O’Toole, G.A. and Kolter, R., Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis, Mol. Microbiol., 1998, vol. 28, no. 3, pp. 449–461.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed, Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989.
Scheludko, A.V., Katsy, E.I., Ostudin, N.A., Gringauz, O.K., and Panasenko, V.I., Novel classes of Azospirillum brasilense mutants with defects in the assembly and functioning of polar and lateral flagella, Mol. Genet. Mikrobiol. Virusol., 1998, no. 4, pp. 33–37.
Schelud’ko, A.V., Makrushin, K.V., Tugarova, A.V., Krestinenko, V.A., Panasenko, V.I., Antonyuk, L.P., and Katsy, E.I., Changes in motility of the rhizobacterium Azospirillum brasilense in the presence of plant lectins, Microbiol. Res., 2009, vol. 164, no. 2, pp. 149–156.
Sheludko, A.V., Kulibyakina, O.V., Shirokov, A.A., Petrova, L.P., Matora, L.Yu., and Katsy, E.I., The effect of mutations affecting synthesis of lipopolysaccharides and calcofluor-binding polysaccharides on biofilm formation by Azospirillum brasilense, Microbiology (Moscow), 2008, vol. 77, no. 3, pp. 313–317.
Shelud’ko, A.V., Ponomareva, E.G., Varshalomidze, O.E., Vetchinkina, E.P., Katsy, E.I., and Nikitina, V.E., Hemagglutinating activity and motility of the bacterium Azospirillum brasilense in the presence of various nitrogen sources, Microbiology (Moscow), 2009, vol. 78, no. 6, pp. 696–702.
Shelud’ko, A.V., Filip’echeva, Y.A., Shumilova, E.M., Khlebtsov, B.N., Burov, A.M., Petrova, L.P., and Katsy, E.I., Changes in biofilm formation in the nonflagellated flhB1 mutant of Azospirillum brasilense Sp245, Microbiology (Moscow), 2015, vol. 84, no. 2, pp. 144–151.
Tarrand, J.X., Krieg, N.E., and Döbereiner, J., A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov., Can. J. Microbiol., 1978, vol. 24, no. 8, pp. 967–980.
Vanderlinde, E.M., Muszynski, A., Harrison, J.J., Koval, S.F., Foreman, D.L., Ceri, H., Kannenberg, E.L., Carlson, R.W., and Yost, C.K., Rhizobium leguminosarum biovar viciae 3841, deficient in 27-hydroxyoctacosanoatemodified lipopolysaccharide, is impaired in desiccation tolerance, biofilm formation and motility, Microbiology (UK), 2009, vol. 155, no. 9, pp. 3055–3069.
Vanderlinde, E.M. and Yost C.K., Genetic analysis reveals links between lipid A structure and expression of the outer membrane protein gene, ropB, in Rhizobium leguminosarum, FEMS Microbiol. Lett., 2012, vol. 335, no. 2, pp. 130–139.
Wisniewski-Dyé, F., Borziak, K., Khalsa-Moyers, G., Alexandre, G., Sukharnikov, L.O., Wuichet, K., Hurst, G.B., McDonald, W.H., Robertson, J.S., Barbe, V., Calteau, A., Rouy, Z., Mangenot, S., Prigent-Combaret, C., Normand, P., Boyer, M., Siguier, P., Dessaux, Y., Elmerich, C., Condemine, G., Krishnen, G., Kennedy, I., Paterson, A.H., Gonzalez, V., Mavingui, P., and Zhulin, I.B., Azospirillum genomes reveal transition of bacteria from aquatic to terrestrial environments, PLoS Genet., 2011, vol. 7, no. 12, p. e1002430.
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Original Russian Text © E.M. Shumilova, A.V. Shelud’ko, Yu.A. Filip’echeva, S.S. Evstigneeva, E.G. Ponomareva, L.P. Petrova, E.I. Katsy, 2016, published in Mikrobiologiya, 2016, Vol. 85, No. 2, pp. 162–170.
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Shumilova, E.M., Shelud’ko, A.V., Filip’echeva, Y.A. et al. Changes in cell surface properties and biofilm formation efficiency in Azospirillum brasilense Sp245 mutants in the putative genes of lipid metabolism mmsB1 and fabG1 . Microbiology 85, 172–179 (2016). https://doi.org/10.1134/S002626171602017X
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DOI: https://doi.org/10.1134/S002626171602017X