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Analysis of proliferation and survival of agrobacteria after inoculation of maize pistil filaments

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Abstract

While the authors have previously developed a method of pistil filament treatment with Agrobacterium cells during blossoming for the transformation of maize generative cells, the mechanism for bacterial T-DNA penetration into the embryo sac remained unknown. This article analyzes the possibility of agrobacterial penetration into the maize embryo via pollen tubes. Microbiological, PCR, and GUS techniques were used to confirm that agrobacteria could spread for up to 20 cm from the site of inoculation and were detected in maize embryo tissues as early as 24 h after inoculation, while they were not revealed after 5–13 days.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia

    I. V. Volokhina, V. A. Velikov, E. M. Moiseeva & M. I. Chumakov

  2. Saratov State University, Saratov, Russia

    V. A. Velikov & M. I. Chumakov

Authors
  1. I. V. Volokhina
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  2. V. A. Velikov
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  3. E. M. Moiseeva
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  4. M. I. Chumakov
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Corresponding author

Correspondence to M. I. Chumakov.

Additional information

Original Russian Text © I.V. Volokhina, V.A. Velikov, E.M. Moiseeva, M.I. Chumakov, 2016, published in Mikrobiologiya, 2016, Vol. 85, No. 1, pp. 66–72.

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Volokhina, I.V., Velikov, V.A., Moiseeva, E.M. et al. Analysis of proliferation and survival of agrobacteria after inoculation of maize pistil filaments. Microbiology 85, 87–92 (2016). https://doi.org/10.1134/S0026261716010148

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