Abstract
Lipopolysaccharides (LPS) from two strains of Pseudomonas chlororaphis subsp. aureofaciens, UCM B-111 and UCM B-306, were isolated and characterized. The LPS preparations exhibited low toxicity, high pyrogenicity, and high antiviral activity. Mild acid hydrolysis was used to obtain the O-specific polysaccharides. Their structures were established by monosaccharide analysis and determination of absolute configurations, as well as by 1D and 2D NMR spectroscopy. The O-polysaccharides were shown to contain the linear trior tetrasaccharide repeating units. Both O-polysaccharides were structurally heterogeneous: P. chlororaphis subsp. aureofaciens UCM B-111 → 4)-α-D-GalpNAc6Ac-(1 → 3)-β-D-QuipNAc-(1 → 6)-α-D-GlcpNAc-(1 →β-D-GlcpNAc-(1 → 3)⎦; degree of the non-stoichiometric 6-O-acetylation of GalNAc ~60%; P. chlororaphis subsp. aureofaciens UCM B-306 → 3)-α-D-Rhap-(1 → 4)-α-D-GalpNAcAN-(1 → 3)-α-D-QuipNAc4NAc-(1 →, where GalNAcAN is 2-acetamido-2-deoxy-D-galacturonamide, the degree of non-stoichiometric amidation of the GalNAcA residue ~60%.
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Original Russian Text © L.D. Varbanets, E.L. Zdorovenko, E.A. Kiprianova, L.V. Avdeeva, O.S. Brovarskaya, S.L. Rybalko, 2015, published in Mikrobiologiya, 2015, Vol. 84, No. 6, pp. 680–690.
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Varbanets, L.D., Zdorovenko, E.L., Kiprianova, E.A. et al. Characterization of the lipipolysaccharides of Pseudomonas chlororaphis . Microbiology 84, 781–790 (2015). https://doi.org/10.1134/S0026261715060132
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DOI: https://doi.org/10.1134/S0026261715060132