Abstract
Immunoelectrophoresis and immunodiffusion analysis with antibodies to whole intact cells of the type strain of nitrogen-fixing soil bacteria Azospirillum brasilense Sp7 revealed at least three conservative surface immunogenic proteins of azospirilla. Cross-reactions with these proteins made it possible to use the above antibodies for the detection of azospirilla as a genus-specific probe, conjugated with horseradish peroxidase as an enzymatic label. Direct immune-enzyme analysis of soil suspensions (typical chernozem, Saratov oblast) confirmed applicability of the conjugates based on genus-specific antibodies to the surface proteins of azospirilla for direct detection of this bacterial genus in environmental samples. These results provide a basis for broad application of this method for the analysis of Azospirillum occurrence in soil.
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Original Russian Text © A.A. Shirokov, A.I. Krasov, N.Yu. Selivanov, G.L. Burygin, S.Yu. Shchyogolev, L.Yu. Matora, 2015, published in Mikrobiologiya, 2015, Vol. 84, No. 2, pp. 244–249.
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Shirokov, A.A., Krasov, A.I., Selivanov, N.Y. et al. Immunochemical detection of Azospirilla in soil with genus-specific antibodies. Microbiology 84, 263–267 (2015). https://doi.org/10.1134/S0026261715020137
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DOI: https://doi.org/10.1134/S0026261715020137