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Rhodobaculum claviforme gen. nov., sp. nov., a new alkaliphilic nonsulfur purple bacterium

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Abstract

Two alkaliphilic strains of nonsulfur purple bacteria (NPB), B7-4 and B8-2, were isolated from moderately saline alkaline steppe lakes in southeast Siberia with pH values above 9.0. The isolates were motile, polymorphous cells (from short rods to long spindly cells) 1–2.5 × 2.5–7 μm. Intracellular membranes of vesicular type were mostly located at the cell periphery. The microorganisms contained bacteriochlorophyll a and carotenoids of the spheroidene and spirilloxanthin series. The photosynthetic apparatus was represented by LH2 and LH1 light-harvesting complexes. In the presence of organic compounds, the strains grew aerobically in the dark or anaerobically in the light. Capacity for photo- and chemoautotrophic growth was not detected. The cbbL gene encoding RuBisCO was not revealed. Optimal growth of both strains occurred at 2% NaCl (range from 0.5 to 4%), pH 8.0–8.8 (range from 7.5 to 9.7), and 25–35°C. The DNA G+C content was 67.6–69.8 mol %. Pairwise comparison of the nucleotides of the 16S rRNA genes revealed that strains B7-4 and B8-2 belonged to the same species (99.9% homology) and were most closely related to the aerobic alkaliphilic bacteriochlorophyll a-containing anoxygenic phototrophic bacterium (APB) Roseibacula alcaliphilum DeT (95.2%) and to NPB strains Rhodobaca barguzinensis VKM B-2406T (94.2%) and Rbc. bogoriensis LBB1T (93.9%). The isolates were closely related to the NPB Rhodobacter veldkampii DSM 11550T (94.8%) and to aerobic bacteriochlorophyll a-containing bacteria Roseinatronobacter monicus ROS 35T and Roseicitreum antarcticul ZS2-28T (93.5 and 93.9%, respectively). New strains were described as a new NPB genus and species of the family Rhodobacteriaceae, Rhodobaculum claviforme gen. nov., sp. nov., with B7-4T (VKM B-2708, LMG 28126) as the type strain.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    I. A. Bryantseva, V. A. Gaisin & V. M. Gorlenko

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  1. I. A. Bryantseva
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  2. V. A. Gaisin
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  3. V. M. Gorlenko
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Correspondence to V. M. Gorlenko.

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Original Russian Text © I.A. Bryantseva, V.A. Gaisin, V.M. Gorlenko, 2015, published in Mikrobiologiya, 2015, Vol. 84, No. 2, pp. 225–235.

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Bryantseva, I.A., Gaisin, V.A. & Gorlenko, V.M. Rhodobaculum claviforme gen. nov., sp. nov., a new alkaliphilic nonsulfur purple bacterium. Microbiology 84, 247–255 (2015). https://doi.org/10.1134/S0026261715020022

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