Abstract
Lipopolysaccharides (LPS) from seven Pantoea agglomerans strains isolated from various plants were purified and chemically identified. LPS of the studied P. agglomerans strains were heterogeneous in monosaccharide composition. Thus, the LPS of P. agglomerans 8606 differed considerably from the LPSs of other strains, containing mannose as the predominant monosaccharide (69.8%), as well as ribose (15.1%) and xylose (12.6%), while the content of rhamnose, one of the predominant monosaccharides in other LPS samples, was 2.5%. Analysis of the fatty acid composition revealed the presence of C12–C16 acids. In lipids A of all the studied strains, 3-OH-C14:0 was the predominant acid (31.7 to 39.1%, depending on the strain). C12:0 (8.2 to 31.5%), C14:0 (12.9 to 30.8%), and C16:0 acids (3.4 to 16.9%) were also revealed. The studied P. agglomerans strains fell into three groups according to their fatty acid composition. The differences stemmed from the presence or absence of two fatty acids, 2-OH-C14:0 and C16:1. Ouchterlony double immunodiffusion in agar revealed that all the LPS under study exhibited antigenic activity in homologous systems. The results of serological cross reactions indicated immunochemical heterogeneity of the species P. agglomerans. Comparative investigation of the complex of parameters of peripheral blood cells from a healthy donor before and after treatment with LPS solutions showed that the values of no parameters exceeded the normal range.
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Weinthal, D.M., Barash, I., Panijel, M., Valinsky, L., Gaba, V., and Manulis-Sasson, S., Distribution and replication of the pathogenicity plasmid pPATH in diverse populations of the gall-forming bacterium Pantoea agglomerans, Appl. Environ. Microbiol., 2007, vol. 73, no. 23, pp. 7552–7561.
Down, J.M., Newman, M.A., and von Roepenack, A., The induction and modulation of plant defense responses by bacterial lipopolysaccharides, Annu. Rev. Phytopathol., 2000, vol. 38, pp. 241–261.
Newman, M.A., Dow, J.M., Molinaro, A., and Parrilli, M., Priming, induction and modulation of plant defense responses by bacterial lipopolysaccharides, J. Endotoxin Res., 2007, vol. 13, pp. 69–84.
Gavini, F.J., Beji, M.A., Mielcarek, C., Izard, D., Kerster, K., and De Ley, J., Transfer of Enterobacter agglomerans (Beijerink 1988) Ewing and File 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov., Int. J. Syst. Bacteriol., 1989, vol. 39, no. 3, pp. 337–345.
Varbanets, L.D., Zdorovenko, G.M., and Knirel’, Yu.A., Metody issledovaniya endotoksinov (Methods of Endotoxin Research), Kiev: Naukova Dumka, 2006.
Sawardeker, J.S., Sloneker, J.H., and Jeans, A., Quantitative determination of monosaccharides as their alditol acetates by gas liquid chromatography, Anal. Chem., 1965, vol. 37, no. 5, pp. 1602–1603.
Ouchterlony, O., Diffusion in gel methods for immunological analysis, Prog. Allergy, 1962, no. 6, pp. 3–15.
Ryabichenko, E.V., Bondarenko, V.M., and Ryabichenko, V.V., Role of phagocyte-generated reactive oxygen species in pathogenesis of diseases, Zh. Mikrobiol. Epidemiol. Immunobiol., 2001, no. 4, pp. 65–71.
Nikitin, V.M., Spravochnik metodov immunologii. (Handbook on Immunological Methods), Chisinau: Shtiintsa, 1982.
Belen’kii, M.L., Elementy kolichestvennoi otsenki farmatsevticheskogo effekta (Elements of Quantitative Assessment of Pharmaceutical Effect), Leningrad: Medtiz, 1963.
Freidlin, I.S. and Totolyan, A.A., Kletki immunnoi sistemy (Cells of the Immune System), St.-Petersburg: Nauka, 2001.
Dolgushin, I.I., Andreeva, Yu.S., and Savochkina, A.Yu., Neitrofil’nye lovushki i metody otsenki funktsional’nogo statusa neitrofilov (Neutrophilic Traps and Methods for Assessment of the Functional Status of Neutrophiles), Moscow: Izd-vo RAMN, 2009, p. 204.
Janeway, C.A. and Medzhitov, R., Innate immune recognition, Annu. Rev. Immunol., 2002, vol. 20, pp. 197–216.
Novikov, D.K. and Novikova, V.I., Otsenka immunnogo statusa (Assessment of Immune Status), Vitebsk, 1996.
Lutsyuk, N.B., Biokhimiya i immunologiya mikrobnykh polisakharidov (Biochemistry and Physiology of Microbial Polysaccharides), Tomsk: Izd. Tomsk. Univ., 1984.
Varbanets, L.D., Zdorovenko, E.A., Garkavaya, E.G., and Brovarskaya, O.S., Lipopolisaccharide of Escherichia coli M-17, Microbiology (Moscow), 2012, vol. 81, no. 3, pp. 324–331.
Borisova, E.V., Investigation of immunobiological properties of the lipopolysaccharides of Shigella spp. bacteria with opposite virulence and of their chemically modified analogues, Mikrobiol. Zh., 1999, vol. 61, no. 4, pp. 64–71.
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Original Russian Text © L.D. Varbanets, O.S. Brovarskaya, T.N. Bulygina, E.G. Garkavaya, N.V. Zhitkevich, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 6, pp. 656–666.
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Varbanets, L.D., Brovarskaya, O.S., Bulygina, T.N. et al. Characterization of Pantoea agglomerans lipopolysaccharides. Microbiology 83, 754–763 (2014). https://doi.org/10.1134/S0026261714060198
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DOI: https://doi.org/10.1134/S0026261714060198