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Production of gaseous hydrocarbons by microbial communities of Lake Baikal bottom sediments

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Abstract

Production of gaseous hydrocarbons by the microbial community of the Posolsky Bank methane seep bottom sediments (southern Baikal) was studied at 4°C. Formation of both methane and a heavier gaseous hydrocarbon, ethane, was detected in enrichment cultures. The highest methane concentrations (6.15 and 4.51 mmol L−1) were revealed in enrichments from the sediments from 55-cm depth incubated with sodium acetate and H2/CO2 gas mixture, respectively. A decrease in activity of aceticlastic methanogens and a decrease in methane concentration produced by hydrogenotrophic archaea occurred with depth. The highest concentration of ethane was revealed in enrichments from the microbial community of the layer close to gas hydrates (75 cm) incubated with CO2 as a substrate. According to analysis of the 16S rRNA gene fragments from the clone library, these enrichments were found to contain members of the phylum Crenarchaeota forming a separate cluster with members of the class Thermoprotei. The phylum Euryarchaeota was represented by nucleotide sequences of the organisms homologous to members of the orders Methanococcales, Methanosarcinales, and Thermoplasmatales.

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Authors and Affiliations

  1. Limnological Institute, Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia

    O. N. Pavlova, S. V. Bukin, A. V. Lomakina, V. G. Ivanov, T. V. Pogodaeva & T. I. Zemskaya

  2. Vinogradov Institute of Geochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia

    G. V. Kalmychkov

  3. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    I. V. Morozov

  4. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    N. V. Pimenov

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  1. O. N. Pavlova
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  2. S. V. Bukin
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  3. A. V. Lomakina
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  4. G. V. Kalmychkov
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  5. V. G. Ivanov
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  6. I. V. Morozov
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  7. T. V. Pogodaeva
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  8. N. V. Pimenov
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  9. T. I. Zemskaya
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Correspondence to O. N. Pavlova.

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Original Russian Text © O.N. Pavlova, S.V. Bukin, A.V. Lomakina, G.V. Kalmychkov, V.G. Ivanov, I.V. Morozov, T.V. Pogodaeva, N.V. Pimenov, T.I. Zemskaya, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 6, pp. 694–702.

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Pavlova, O.N., Bukin, S.V., Lomakina, A.V. et al. Production of gaseous hydrocarbons by microbial communities of Lake Baikal bottom sediments. Microbiology 83, 798–804 (2014). https://doi.org/10.1134/S0026261714060137

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