Abstract
Well-known yeasts Kluyveromyces lactis var. lactis, which are usually associated with dairy products, were discovered in nature: in woodland park soil under Impatiens glandulifera Royle plants. Reliable identification of the yeasts was carried out using physiological criteria (lactose and maltose utilization) and molecular markers (nucleotide sequence of the 5.8S-ITS rDNA fragment, pulsed-field gel electrophoresis, and Southern hybridization of chromosomal DNA with the LAC4 probe). Ecology of Kl. lactis var. lactis is discussed.
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Wésolowski-Louvel, M., Breunig, K.D., and Fukuhara, H., Kluyveromyces lactis, in Nonconventional Yeasts in Biotechnology. A Handbook, Berlin: Springer, 1996, pp. 139–201.
Bolotin-Fukuhara, M., Toffano-Nioche, C., Artiguenave, F., Duchateau-Nguyen, G., Lemaire, M., Marmeisse, R., Montrocher, R., Robert, C., Termier, M., Wincker, P., and Wésolowski-Louvel, M., Genomic exploration of the hemiascomycetous yeasts: 11. Kluyveromyces lactis, FEBS Lett., 2000, vol. 487, pp. 66–70.
Schaffrath, R. and Breunig, K.D., Genetics and molecular physiology of the yeast Kluyveromyces lactis, Fungal Genet. Biol., 2002, vol. 30, pp. 173–190.
Breunig, K.D. and Steensma, H.Y., Kluyveromyces lactis: genetics, physiology and application, in Functional Genetics of Industrial Yeasts, vol. 2. Topics in Current Genetics, Berlin: Springer, 2003, pp. 171–205.
Rubio-Texeira, M., Endless versatility in the biotechnological applications of Kluyveromyces LAC genes, Biotechnol. Advanc., 2006, vol. 24, pp. 212–225.
Fukuhara, H., Kluyveromyces lactis—a retrospective, FEMS Yeast Res., 2006, vol. 6, no. 3, pp. 323–324.
Naumov, G.I. and Naumova, E.S., Five new combinations in the yeast genus Zygofabospora Kudriavzev emend. G. Naumov (pro parte Kluyveromyces) based on genetic data, FEMS Yeast Res., 2002, vol. 2, no. 1, pp. 39–46.
Naumova, E.S., Sukhotina, N.N., and Naumov, G.I., Molecular genetic differentiation of the dairy yeast Kluyveromyces lactis and its closest wild relatives, FEMS Yeast Res., 2004, vol. 5, no. 3, pp. 263–269.
Naumov, G.I., Zygofabospora krassilnikovii, a wild European species, is an ancestor of the dairy yeasts Z. lactis, Dokl. Biol. Sci., 2000, vol. 372, no. 6, pp. 421–424.
Naumov, G.I., Naumova, E.S., Barrio, E., and Querol, A., Genetic and molecular study of the inability of the yeast Kluyveromyces lactis var. drosophilarum to ferment lactose, Microbiology (Moscow), 2006, vol. 75, no. 3, pp. 248–252.
Naumov, G.I., Naumova, E.S., and Choi, E.-S., Natural and industrially important patterns of sugar utilization in the yeast Kluyveromyces marxianus, Biotekhnologiya, 2010, no. 2, pp. 54–58.
Naumov, G.I., Genetics of lactose utilization polymorphism in the yeast Kluyveromyces marxianus, Dokl. Biol. Sci, 2006, vol. 409, no. 3, pp. 317–319.
Naumov, G.I., Why does the yeast Kluyveromyces wickerhamii assimilates but not ferments lactose?, Dokl. Biol. Sci., 2005, vol. 403, no. 6, pp. 310–312.
Glushakova, A.M., Kachalkin, A.V., and Chernov, I.Yu., Specific features of the dynamics of epiphytic and soil yeast communities in the thickets of Indian balsam on mucky gley soil, Euras. Soil Sci., 2011, vol. 44, no. 8, pp. 886–892.
Kurtzman, C.P., Fell, J.W., Boekhout, T., and Robert, V., Methods for isolation, phenotypic characterization and maintenance of yeasts, in The Yeasts, A Taxonomic Study, Kurtzman, C.P., Fell, J.W., and Boekhout, T., Eds., Amsterdam: Elsevier, 2011, pp. 87–110.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol., 2011, vol. 28, pp. 2731–2739.
Naumova, E.S., Sukhotina, N.N., and Naumov, G.I., Molecular markers for differentiation between the closely related dairy yeast Kluyveromyces lactis var. lactis and wild Kluyveromyces lactis strains from the European “krassilnikovii” population, Microbiology (Moscow), 2005, vol. 74, no. 3, pp. 329–335.
Adam, A.C., Prieto, J.A., Rubio-Texeira, M., and Polaina, J., Construction of a lactose-assimilating strain of baker’s yeast, Yeast, 1999, vol. 15, pp. 1299–1305.
Lachance, M.-A., Kluyveromyces van der Walt (1971), in The Yeasts, A Taxonomic Study, Kurtzman, C.P., Fell, J.W., and Boekhout, T., Eds., Amsterdam: Elsevier, 2011, pp. 471–482.
Naumova, E.S., Naumov, G.I., Nikitina, T.N., Sadykova, A.Zh., and Kondratieva, V.I., Molecular genetic and physiological differentiation of Kluyveromyces lactis and Kluyveromyces marxianus: analysis of strains from the All-Russian collection of microorganisms (VKM), Microbiology (Moscow), 2012, vol. 81, no. 2, pp. 216–223.
Wésolowski-Louvel, M., Breunig, K.D., and Fukuhara, H., Kluyveromyces lactis, in Nonconventional Yeasts in Biotechnology: A Handbook, Wolf, K.B., Ed., Berlin: Springer, 1996, pp. 139–201.
Naumov, G.I. and Naumova, E.S., Polymeric lactose fermentation genes in the yeast Kluyveromyces lactis: a new locus LAC3, Dokl. Biol. Sci., 2014, vol. 435, pp. 106–108.
Naumov, G.I., Identification of the lactose LAC gene superfamilies in Kluyveromyces yeast, Dokl. Biochem. Biophys, 2008, vol. 420, no. 6, pp. 158–160.
Naumov, G.I., Domestication of dairy yeast Kluyveromyces lactis: transfer of the β-galactosidase (LAC4) and lactose permease (LAC12) gene cluster?, Dokl. Biol. Sci., 2005, vol. 401, no. 2, pp. 120–123.
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Original Russian Text © G.I. Naumov, E.S. Naumova, A.M. Glushakova, A.V. Kachalkin, I.Yu. Chernov, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 6, pp. 677–681.
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Naumov, G.I., Naumova, E.S., Glushakova, A.M. et al. Finding of dairy yeasts Kluyveromyces lactis var. lactis in natural habitats. Microbiology 83, 782–786 (2014). https://doi.org/10.1134/S0026261714060125
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DOI: https://doi.org/10.1134/S0026261714060125