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Comparison of biochemical and molecular genetic approaches for identification of environmental strains

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Abstract

In order to confirm the taxonomic position of environmental strains determined based on their biochemical, cultural, and morphological characteristics, molecular genetic identification was carried out. A number of problems in identification of microorganisms were shown to be associated with contamination of the cultures in the course of isolation. Advantages of a comprehensive approach—combining 16S rRNA gene sequencing with a set of biochemical, cultural, and morphological parameters—for identification of microorganisms isolated from environmental objects and clinical samples are discussed.

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Authors and Affiliations

  1. Southern Federal University, ul. Bolshaya Sadovaya 105/42, Rostov-on-Don, 344006, Russia

    S. O. Korshunov, A. V. Gorovtsov, S. N. Gorbov & I. V. Kornienko

  2. Rostov State Medical University, Nakhichevanskiy per. 29, Rostov-on-Don, 344022, Russia

    T. G. Faleeva

  3. 16 State Center for Forensic Medical and Criminal Expertise, Southern Military District, the Ministry of Defense of the Russian Federation, ul. Lermontovskaya 60, Rostov-on-Don, 344010, Russia

    T. G. Faleeva & I. V. Kornienko

Authors
  1. S. O. Korshunov
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  2. A. V. Gorovtsov
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  3. T. G. Faleeva
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  4. S. N. Gorbov
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  5. I. V. Kornienko
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Corresponding author

Correspondence to A. V. Gorovtsov.

Additional information

Original Russian Text © S.O. Korshunov, A.V. Gorovtsov, T.G. Faleeva, S.N. Gorbov, I.V. Kornienko, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 4, pp. 451–455.

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Korshunov, S.O., Gorovtsov, A.V., Faleeva, T.G. et al. Comparison of biochemical and molecular genetic approaches for identification of environmental strains. Microbiology 83, 376–380 (2014). https://doi.org/10.1134/S0026261714040109

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  • DOI: https://doi.org/10.1134/S0026261714040109

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