Abstract
The putative METDI2644 (modA 2 ) gene of Methylobacterium dichloromethanicum DM4, present in the 126-kb chromosomal fragment associated with dichloromethane (DCM) degradation, was investigated. While this gene is presumed to encode the periplasmic substrate-binding subunit of a molybdate ABC transporter, its conceptual translation also exhibits similarity to proteins containing the ostA conservative domain and responsible for resistance of gram-negative bacteria to organic solvents. Reverse transcription polymerase chain reaction (RT-PCR) revealed RNA transcripts of this gene in the cells grown on either DCM or cells grown on methanol. The mobilizable suicide vector pK18mob was used to obtain a knockout mutant with the METDI2644 gene inactivated by insertion of the gentamycin cassette. The mutant pregrown on methanol exhibited lower growth rate on DCM than the wild-type strain DM4. The difference was not alleviated by the addition of sodium molybdate. Our results demonstrate that the METDI2644 gene product plays a role in cell adaptation to DCM degradation.
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Original Russian Text © Y.E. Firsova, Y.A. Trotsenko, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 3, pp. 295–301.
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Firsova, Y.E., Trotsenko, Y.A. Functional activity of the modA 2 gene in Methylobacterium dichloromethanicum DM4. Microbiology 83, 205–210 (2014). https://doi.org/10.1134/S0026261714030060
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DOI: https://doi.org/10.1134/S0026261714030060