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Characterization of the lipopolysaccharide from Budvicia aquatica DLR 20186

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Abstract

A lipopolysaccharide (LPS) from Budvicia aquatica DRL 20186 was isolated, studied, and chemically identified. It was shown to be lowly toxic, but highly pyrogenic. Its fatty acid composition was similar to that of the LPS from other Enterobacteriaceae, with predominance of tetradecanoic (32.7%) and 3-hydroxytetradecanoic acids (23.8%). Hexadecenoic (20.4%), hexadecanoic (11.8%), and dodecanoic acids (8.4%) were also revealed. Double immunodiffusion in agar by the Ouchterlony method revealed antigenic activity of the B. aquatica DLR 20186 LPS in a homologous system. In cross reactions, however, it did not interact with the antisera of other B. aquatica strains.

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Authors and Affiliations

  1. Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences, Kiev, Ukraine

    L. D. Varbanets & O. S. Brovarskaya

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

    E. L. Zdorovenko

  3. Mechnikov Institute of Microbiology and Immunology, National Academy of Medical Sciences, Kharkov, Ukraine

    S. I. Pokhil

Authors
  1. L. D. Varbanets
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  2. E. L. Zdorovenko
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  3. O. S. Brovarskaya
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  4. S. I. Pokhil
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Correspondence to L. D. Varbanets.

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Original Russian Text © L.D. Varbanets, E.L. Zdorovenko, O.S. Brovarskaya, S.I. Pokhil, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 1, pp. 49–55.

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Varbanets, L.D., Zdorovenko, E.L., Brovarskaya, O.S. et al. Characterization of the lipopolysaccharide from Budvicia aquatica DLR 20186. Microbiology 82, 778–783 (2013). https://doi.org/10.1134/S0026261714010160

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  • DOI: https://doi.org/10.1134/S0026261714010160

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