Skip to main content
SpringerLink
Log in

Effect of silver nanoparticles on the parameters of chlorophyll fluorescence and P700 reaction in the green alga Chlamydomonas reinhardtii

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

Acute toxicity of silver nanoparticle (AgNP) for photosynthesis in Chlamydomonas reinhardtii was studied using an M-PEA2 fluorimeter. Analysis of the fluorescence induction curves in the presence of AgNP at low concentrations revealed inhibited electron transport on the PS2 photosystem and increased content of QB-nonreducing centers. No direct effect of AgNP on the reactions of P700 oxidation in PS1 was found, while the energization of the photosynthetic membranes was affected. Investigation of the parameters of the prompt and delayed fluorescence is proposed as a method for early detection of AgNP in the environment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AgNP:

silver nanoparticles

PS2:

photosystem 2

RC:

reaction center

QA and QB :

primary and secondary quinone electron acceptors

PQ:

plastoquinone

O, J, I, and P:

intermediate stages of light-induced fluorescence curve

DF:

delayed fluorescence

P700 :

pigment of the RC in PS1

References

  1. Lee, H.Y., Park, H.K., Lee, Y.M., and Park, S.B., A practical procedure for producing silver nanocoated fabric and its antibacterial evaluation for biomedical applications, Chem. Commun., 2007, vol. 28, pp. 2959–2961.

    Article  Google Scholar 

  2. Choi, O., Deng, K.K., Kim, N.J., Ross, L., Jr., and Surampalli, R.Y., The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth, Water Res., 2008, vol. 42, pp. 3066–3074.

    Article  CAS  PubMed  Google Scholar 

  3. Wiesner, M.R., Lowry, G.V., Alvarez, P., Dionysiou, D., and Biswas, P., Assessing the risks of manufactured nanomaterials, Environ. Sci. Technol., 2006, vol. 40, pp. 4336–4345.

    Article  CAS  PubMed  Google Scholar 

  4. Matorin, D.N. and Rubin, A.B., Fluorestsentsii khlorofilla vysshikh rastenii i vodoroslei (Chlorophyll Fluorescence in Algae and Higher Plants), Moscow-Izhevsk: IKI-RKhD, 2012.

    Google Scholar 

  5. Hiriart-Baer, V.P., Lee, D.Y., and Campbell, P.G., Toxicity of silver to two freshwater algae Chlamydomonas reinhardtii and Pseudokirchneriella subcapitata grown under continuous culture conditions: Influence of thiosulphate, Aquat. Toxicol., 2006, vol. 78, pp. 136–148.

    Article  CAS  PubMed  Google Scholar 

  6. Dmitrieva, A.G., Boichuk, T.V., and Filenko, O.F., Population heterogeneity in Scenedesmus quadricauda under different modes of silver intoxication, Ekol. Pribory Sistemy, 2007, no. 3, pp. 42–45.

    Google Scholar 

  7. Oukarroum, A., Perreault, F., Bras, S., and Popovic, R., Inhibitory effects of silver nanoparticles in two green algae, Chlorella vulgaris and Dunaliella tertiolecta, Ecotoxicol. Environ. Safety, 2012, vol. 78, pp. 80–85.

    Article  CAS  PubMed  Google Scholar 

  8. Navarro, E., Piccapietra, F., Wagner, B., Kogi, R., Odzak, N., Sigg, L., and Behra, R., Toxicity of silver nanoparticles to Chlamydomonas reinhardtii, Environ. Sci. Technol., 2008, vol. 42, pp. 8959–8964.

    Article  CAS  PubMed  Google Scholar 

  9. Matorin, D.N., Karateeva, A.V., Osipov, V.A., Lukashev, E.P., Seifullina, N.Kh., and Rubin A.B., Influence of carbon nanotubes on chlorophyll fluorescence parameters of green algae Chlamydomonas reinhardtii, Nanotechnologies in Russia, 2010, nos. 5–6, pp. 320–327.

    Google Scholar 

  10. Matorin, D.N., Osipov, V.A., Seifullina, N.Kh., Venediktov, P.S., and Rubin, A.B., Increasing toxic effect of methylmercury on Chlorella vulgaris under high light and cold stress conditions, Microbiology (Moscow), 2009, vol. 78, no. 3, pp. 321–327.

    Article  CAS  Google Scholar 

  11. Strasser, R.J., Tsimilli-Michael, M., Qiang, S., and Goltsev, V., Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis, Biochim. Biophys. Acta, 2010, vol. 1797, nos. 6–7, pp. 1313–1326.

    Article  CAS  PubMed  Google Scholar 

  12. Bulychev, A.A., Osipov, V.A., Matorin, D.N., and Vredenberg, W.J., Effects of far-red light on fluorescence induction in infiltrated pea leaves under diminished ΔpH and Δφ components of the proton motive force, J. Bioenerg. Biomembr., 2013, vol. 45, no. 1, pp. 37–45.

    Article  CAS  PubMed  Google Scholar 

  13. Antal, T.K., Osipov, V.A., Matorin, D.N., and Rubin, A.B., Membrane potential is involved in regulation of photosynthetic reactions in the marine diatom Thalassiosira weissflogii, J. Photochem. Photobiol., Ser. B: Biol., 2011, vol. 102, pp. 169–173.

    Article  CAS  Google Scholar 

  14. Bulychev, A.A., Induction changes in photosystem I and II in plant leaves upon modulation of membrane ion transport, Biochemistry (Moscow) Suppl. Ser. A: Membr. Cell Biol., 2011, vol. 5, no. 4, pp. 335–342.

    Google Scholar 

  15. Goltsev, V., Zaharieva, I., Chernev, P., and Strasser, R.J., Delayed fluorescence in photosynthesis, Photosynth. Res., 2009, vol. 101, pp. 217–232.

    Article  CAS  PubMed  Google Scholar 

  16. Kim, J.S., Kuk, E., Yu, K.N., Kim, J.H., Park, S.J., Lee, H.J., Kim, S.H., Park, Y.K., Park, Y.H., Hwang, C.Y., Kim, Y.K., Lee, Y.S., Jeong, D.H., and Cho, M.H., Antimicrobial effects of silver nanoparticles, Nanomed. Nanotechnol. Biol. Med., 2007, vol. 3, pp. 95–101.

    Article  CAS  Google Scholar 

  17. Pal, S., Tak, Y.K., and Song, J.M., Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli, Appl. Environ. Microbiol., 2007, vol. 73, pp. 1712–1720.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Yamanaka, M. Hara, K., and Kudo, J., Bactericidal actions of a silver ion solution on Escherichia coli, studied by energy-filtering transmission electron microscopy and proteomic analysis, Appl. Environ. Microbiol., 2005, vol. 71, pp. 7589–7593.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Brunner, T.J., Wick, P., Manser, P., Spohn, P., Grass, R.N., Limbach, L.K., Bruinink, A., and Stark, W.J., In vitro cytotoxicity of oxide nanoparticles: comparison to asbestos, silica, and the effect of particle solubility, Environ. Sci. Technol., 2006, vol. 40, pp. 4374–4381.

    Article  CAS  PubMed  Google Scholar 

  20. Franklin, N., Rogers, N., Apte, S., Batley, G., Gadd, G., and Casey, P., Comparative toxicity of nanoparticulate ZnO, bulk ZnO, and ZnCl2 to a freshwater microalga (Pseudokirchneriella subcapitata): the importance of particle solubility, Environ. Sci. Technol., 2007, vol. 41, pp. 8484–8490.

    Article  CAS  PubMed  Google Scholar 

  21. Heinlaan, M., Ivask, A., Blinova, I., Dubourguier, H.C., and Kahru A., Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus, Chemosphere, 2008, vol. 71, pp. 1308–1316.

    Article  CAS  PubMed  Google Scholar 

  22. Aruoja, V., Dubourguier, H.C., Kasemets, K., and Kahru, A., Toxicity of nanoparticles of ZnO, CuO and TiO2 to microalgae Pseudokirchneriella subcapitata, Sci. Total Environ., 2009, vol. 407, no. 4, pp. 1461–1468.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Biology, Moscow State University, Moscow, Russia

    D. N. Matorin, D. A. Todorenko, N. Kh. Seifullina & A. B. Rubin

  2. Al-Farabi Kazakhstan National University, Alma-Aty, Kazakhstan

    B. K. Zayadan

Authors
  1. D. N. Matorin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. A. Todorenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. Kh. Seifullina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. K. Zayadan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. B. Rubin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. N. Matorin.

Additional information

Original Russian Text © D.N. Matorin, D.A. Todorenko, N.Kh. Seifullina, B.K. Zayadan, A.B. Rubin, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 1, pp. 33–40.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matorin, D.N., Todorenko, D.A., Seifullina, N.K. et al. Effect of silver nanoparticles on the parameters of chlorophyll fluorescence and P700 reaction in the green alga Chlamydomonas reinhardtii . Microbiology 82, 809–814 (2013). https://doi.org/10.1134/S002626171401010X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S002626171401010X

Keywords

Search

Navigation