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Culturable psychrotolerant methanotrophic bacteria in landfill cover soil

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Abstract

Methanotrophs closely related to psychrotolerant members of the genera Methylobacter and Methylocella were identified in cultures enriched at 10°C from landfill cover soil samples collected in the period from April to November. Mesophilic methanotrophs of the genera Methylobacter and Methylosinus were found in cultures enriched at 20°C from the same cover soil samples. A thermotolerant methanotroph related to Methylocaldum gracile was identified in the culture enriched at 40°C from a sample collected in May (the temperature of the cover soil was 11.5–12.5°C). In addition to methanotrophs, methylobacteria of the genera Methylotenera and Methylovorus and members of the genera Verrucomicrobium, Pseudomonas, Pseudoxanthomonas, Dokdonella, Candidatus Protochlamydia, and Thiorhodospira were also identified in the enrichment cultures. A methanotroph closely related to the psychrotolerant species Methylobacter tundripaludum (98% sequence identity of 16S rRNA genes with the type strain SV96T) was isolated in pure culture. The introduction of a mixture of the methanotrophic enrichments, grown at 15°C, into the landfill cover soil resulted in a decrease in methane emission from the landfill surface in autumn (October, November). The inoculum used was demonstrated to contain methanotrophs closely related to Methylobacter tundripaludum SV96.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    A. Yu. Kallistova & A. N. Nozhevnikova

  2. Department of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, Biocenter 1A, Viikinkaari 9, Helsinki, FIN-00014, Finland

    L. Montonen

  3. Department of Biotechnology and Chemical Technology, Aalto University School of Chemical Technology, P.O. Box 16100, Aalto, Kemistintie 1, Espoo, FIN-00076, Finland

    G. Jurgens

  4. Institute of Environmental Engineering and Biotechnology, Tampere University of Technology, P.O. Box 541, Korkeakoulunkatu 8, Tampere, FIN-33101, Finland

    U. Münster

  5. Engineering and Technology Center OJSC Mosvodokanal, Pervyi Kur’yanovskii proezd 15, Moscow, 109235, Russia

    M. V. Kevbrina

Authors
  1. A. Yu. Kallistova
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  2. L. Montonen
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  3. G. Jurgens
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  4. U. Münster
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  5. M. V. Kevbrina
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  6. A. N. Nozhevnikova
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Correspondence to A. Yu. Kallistova.

Additional information

Original Russian Text © A.Yu. Kallistova, L. Montonen, G. Jurgens, U. Münster, M.V. Kevbrina, A.N. Nozhevnikova, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 1, pp. 109–118.

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Kallistova, A.Y., Montonen, L., Jurgens, G. et al. Culturable psychrotolerant methanotrophic bacteria in landfill cover soil. Microbiology 82, 847–855 (2013). https://doi.org/10.1134/S0026261714010044

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