Skip to main content
SpringerLink
Log in

Phosphate-solubilizing activity of aerobic methylobacteria

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

Phosphate-solubilizing activity was found in 14 strains of plant-associated aerobic methylobacteria belonging to the genera Methylophilus, Methylobacillus, Methylovorus, Methylopila, Methylobacterium, Delftia, and Ancyclobacter. The growth of methylobacteria on medium with methanol as the carbon and energy source and insoluble tricalcium phosphate as the phosphorus source was accompanied by a decrease in pH due to the accumulation of up to 7 mM formic acid as a methanol oxidation intermediate and by release of 120–280 μM phosphate ions, which can be used by both bacteria and plants. Phosphate-solubilizing activity is a newly revealed role of methylobacteria in phytosymbiosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mahidi, S.S., Hassan, G.I., Hussain, A., and Faisul-ur-Rasool, Phosphorus availability issue—its fixation and role of phosphate solubilizing bacteria in phosphate solubilization—case study, Res. J. Agric. Sci., 2011, vol. 2, pp. 174–179.

    Google Scholar 

  2. Ahmed, N. and Shahab, S., Phosphate solubilization: their mechanism, genetics and application, The Internet J. Microbiol., 2011, vol. 9, no. 1, doi: 10.5580/2327

    Google Scholar 

  3. Cuningham, J.E. and Kuiak, C., Production of citric and oxalic acid and solubilization of calcium phosphate by Penicillium billai, Appl. Environ. Microbiol., 1992, vol. 58, pp. 1451–1458.

    Google Scholar 

  4. Uzair, B. and Ahmed, N., Screening of phytate hydrolyzing marine bacteria isolated from Balochistan coast, J. Basic. Appl. Sci., 2006, vol. 3, pp. 19–23.

    Google Scholar 

  5. Azcon-Aguilar, C., Gianinazzi-Pearson, V., Fardeau, J.F., and Gianinazzi, S., Effect of vesicular-arbuscular mycorrhizal fungi and phosphate solubilizing bacteria on growth and nutrition of soybean in a neutral calcareous soil amended with 32P-45Ca-tricalcium phosphate, Plant Soil, 1986, vol. 96, pp. 3–15.

    Article  CAS  Google Scholar 

  6. Song, O.R., Lee, S.J., Lee, Y.S., Lee, S.C., Kim, K.K., and Choi, Y.L., Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil, Brazil. J. Microbiol., 2008, vol. 39, pp. 151–156.

    Article  Google Scholar 

  7. Lapeyrie, F., Ranger, J., and Varelles, D., Phosphate solubilizing activity of ectomycorrhizal fungi in vitro, Can. J. Bot., 1991, vol. 69, pp. 342–346.

    Article  CAS  Google Scholar 

  8. Illmer, P. and Schineer, F., Solubilization of insoluble phosphates by microrganisms isolated from forest soils, Soil Biol. Biochem., 1992, vol. 24, pp. 389–395.

    Article  Google Scholar 

  9. Fasim, F., Ahmed, N., Parsons, R., and Gadd, G.M., Solubilization of zinc salts by bacterium isolated by the air environment of tannery, FEMS Microbiol. Lett., 2002, vol. 213, pp. 1–6.

    Article  CAS  PubMed  Google Scholar 

  10. Kim, K.Y., Jordan, D., and Krishnan, H.B., Rahnella aqualitis, bacterium isolated from soybean rhizosphere, can solubilize hydroxyapatite, FEMS Microbiol. Lett., 1997, vol. 153, pp. 273–277.

    Article  CAS  Google Scholar 

  11. Corpe, W.A. and Basile, D.V., Methanol-utilizing bacteria associated with green plants, Dev. Ind. Microbiol., 1982, vol. 23, pp. 483–493.

    Google Scholar 

  12. Holland, M.A., Methylobacterium and plants, Recent Res. Devel. Plant Physiol., 1997, vol. 1, pp. 207–213.

    Google Scholar 

  13. Trotsenko, Yu.A., Doronina, N.V., and Torgonskaya, M.L., Aerobnye metilobakterii (Aerobic Methylobacteria), Gal’chenko, V.F., Ed., Pushchino: ONTI PSC RAS, 2010.

  14. Doronina, N.V. and Trotsenko, Y.A., A novel plantassociated thermotolerant alkaliphilic methylotroph of the genus Paracoccus, Microbiology (Moscow), 2000, vol. 69, pp. 593–598.

    Article  CAS  Google Scholar 

  15. Doronina, N.V., Kudinova, L.V., and Trotsenko, Yu.A., Methylovorus mays sp. nov.—a new species of aerobic obligately methylotrophic bacteria associated with plants, Microbiology (Moscow), 2000, vol. 69, pp. 599–603.

    Article  CAS  Google Scholar 

  16. Fedorov, D.N., Doronina, N.V., and Trotsenko, Yu.A., Phytosymbiosis of aerobic methylobacteria: new facts and views, Microbiology (Moscow), 2011, vol. 80, no. 4, pp. 443–454.

    Article  CAS  Google Scholar 

  17. MacDonald, R. and Fall, R., Detection of substantial emissions of methanol from plants to the atmosphere, Atmos. Environ., 1993, vol. 27A, pp. 1709–1713.

    Article  CAS  Google Scholar 

  18. Fall, R., Cycling of methanol between plants, methylotrophs and the atmosphere, in Microbial Growth on C1-Compounds, Lidstrom, M.E. and Tabita, F.R., Eds., Dordrecht: Kluwer Acad., 1996, pp. 343–350.

    Chapter  Google Scholar 

  19. Trotsenko, Yu.A., Ivanova, E.G., and Doronina, N.V., Aerobic methylotrophic bacteria as phytosymbionts, Microbiology (Moscow), 2001, vol. 70, no. 6, pp. 623–632.

    Article  CAS  Google Scholar 

  20. Doronina, N.V., Kaparullina, E.N., and Trotsenko, Yu.A., Methylovorus menthalis, a novel species of aerobic obligate methylobacteria associated with plants, Microbiology (Moscow), 2011, vol. 80, no. 5, pp. 713–719.

    Article  CAS  Google Scholar 

  21. Eshinimaev, B.Ts., Khmelenina, V.N., Sakharovskii, V.G., Suzina, N.E., and Trotsenko, Yu.A., Physiological, biochemical, and cytological characteristics of a haloalkalotolerant methanotroph grown on methanol, Microbiology (Moscow), 2002, vol. 71, no. 5, pp. 512–518.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia

    N. V. Agafonova, E. N. Kaparullina, N. V. Doronina & Yu. A. Trotsenko

  2. Pushchino State Institute of Natural Sciences, pr. Nauki 3, Pushchino, Moscow oblast, 142290, Russia

    N. V. Agafonova, E. N. Kaparullina, N. V. Doronina & Yu. A. Trotsenko

Authors
  1. N. V. Agafonova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. N. Kaparullina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. V. Doronina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu. A. Trotsenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu. A. Trotsenko.

Additional information

Original Russian Text © N.V. Agafonova, E.N. Kaparullina, N.V. Doronina, Yu.A. Trotsenko, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 1, pp. 28–32.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Agafonova, N.V., Kaparullina, E.N., Doronina, N.V. et al. Phosphate-solubilizing activity of aerobic methylobacteria. Microbiology 82, 864–867 (2013). https://doi.org/10.1134/S0026261714010020

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261714010020

Keywords

Search

Navigation