Abstract
A strain of Kluyveromyces lactis was found to secrete a fungicidal mycocin active in the pH range from 6 to 9 and exhibiting the highest activity at pH of approximately 7. A few yeast species of the families Saccharomycetaceae and Wickerhamomycetaceae were sensitive to the mycocin. Some genera and species were heterogeneous in this respect. UV treatment of the mycocinogenic strain resulted in loss of its antifungal activity. Although prokaryotes were not sensitive to the mycocin, the strain under study inhibited growth of some bacteria.
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Golubev W.I. and Golubev N.V. Selection and study of potent lactose-fermenting yeasts, Appl. Biochem. Microbiol., 2004, vol. 40, no. 3, pp. 280–284.
van Ooyen, A.J.J., Dekker, P., Huang, M., Olsthoorn, M.M.A., Jacobs, D.I., Colussi, P.A., and Taron, C.H., Heterologous protein production in the yeast Kluyveromyces Lactis, FEMS Yeast Res., 2006, vol. 6, pp. 381–392.
Gunge, N., Tamaru, A., Ozawa, F., and Sakaguchi, K., Isolation and characterization of liner deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character, J. Bacteriol., 1981, vol. 145, no. 1, pp. 382–390.
Nesterova, G.F., Killer system in Kluyveromyces lactis yeast, Genetika, 1989, vol. 25, no. 1, pp. 5–14.
Stark, M.J.R., Boyd, A., Mileham, A.J., and Romanos, M.A., The plasmid-encoded killer system of Kluyveromyces lactis: A review, Yeast, 1990, vol. 6, no. 1, pp. 1–29.
Golubev, W.I., Action spectrum of Kluyveromyces lactis mycocins, Microbiology, 2013, vol. 82, no. 1, pp. 77–84.
Golubev, W.I., Mycocinotyping, Mikol. Fitopatol., 2012, vol. 46, no. 1, pp. 3–13.
Sugisaki, Y., Gunge, N., Sakaguchi, K., Yamasaki, M., and Tamura, G., Characterization of a novel killer toxin encoded by a double-stranded linear DNA plasmid of Kluyveromyces lactis, Eur. J. Biochem., 1984, vol. 141, pp. 241–245.
Klassen, R., Jablonowski, D., Stark, M.J.R., Schaffrath, R., and Meinhardt, F., Mating-type locus control of killer toxins from Kluyveromyces lactis and Pichia acaciae, FEMS Yeast Res., 2006, vol. 6, no. 3, pp. 404–413.
Izgu, F. and Altinbay, D., Killer toxins of certain yeast strains have potential growth inhibitory activity on Gram-positive pathogenic bacteria, Microbios, 1997, vol. 89, no. 358, pp. 15–22.
Fuentefria, A.M., Perez, L.R.R., Azevedo, P.A., Pazzini, F., Schrank, A., Vainstein, M.H., and Valente, P., Typing of Staphylococcus epidermidis clinical strains by a selected panel of Brazilian killer yeasts, J. Bas. Microbiol., 2008, vol. 48, no. 1, pp. 25–30.
Meneghin, M.C., Reis, V.R., and Ceccato-Antonini, S.R., Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts, Braz. Arch. Biol. Technol., 2010, vol. 53, no. 5, pp. 1043–1050.
Gales, A.C., Jones, R.N., Forward, K.R., Linares, J., Sader, H.S., and Verhoel, J., Emerging importance of multidrag-resistant Acinetobacter species and Stenotrophomonas maltophila as pathogens in seriously ill patients: geographic patterns, epidemiological features, and trends in the SENTRY antimicrobial surveillance program (1997–1999), Clin. Infect. Dis., 2001, vol. 32,Suppl. 2, pp. 104–113.
Khodzhakuliev, G.K., Effect of chal on the secretory and motoric function of the stomach, Tr. Ashkhabad. NII Epidemiol. Gigieny, 1964, vol. 6, pp. 355–359.
Khodzhakuliev, G.K., Artamonova, A.G., Dvornikova, I.M., and Egorova, N.M., Effect of chal, a cultured milk product, on blood cholesterol fractions, Tr. Ashkhabad. NII Epidemiol. Gigieny, 1964, vol. 6, pp. 365–369.
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Original Russian Text © W.I. Golubev, 2013, published in Mikrobiologiya, 2013, Vol. 82, No. 3, pp. 295–299.
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Golubev, W.I. A Kluyveromyces lactis mycocin active at neutral pH. Microbiology 82, 290–294 (2013). https://doi.org/10.1134/S0026261713030065
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DOI: https://doi.org/10.1134/S0026261713030065