Abstract
Microbiological investigation of the highly mineralized, sulfide-rich cold spring of the Ust’-Kachka resort was carried out. The total number and biomass of microbial cells were 50 × 103 cell/mL and 15 μg/L, respectively. While the total microbial number was low, the sulfate reduction rate determined by the radioisotope method was relatively high (0.575 mg S L−1 day−1). An enrichment culture was obtained on Widdel medium. According to the results of cloning the 16S rRNA gene fragments with subsequent restriction analysis, the dominant organisms were group 6 sulfate-reducing bacteria (Desulfovibrio-Desulfomicrobium) and the microorganism exhibiting 99% similarity to the anaerobic haloalkaliphilic bacterium Halanaerobium hydrogeniformans.
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Original Russian Text © N.V. Pimenov, G.V. Kuranov, A.L. Bryukhanov, E.F. Veslopolova, I.P. Koryukina, Yu.N. Maslov, 2012, published in Mikrobiologiya, 2012, Vol. 81, No. 6, pp. 779–785.
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Pimenov, N.V., Kuranov, G.V., Bryukhanov, A.L. et al. The sulfate-reducing bacterial community of sulfide-rich water of the Ust’-Kachka resort spring, Perm Krai, Russia. Microbiology 81, 721–726 (2012). https://doi.org/10.1134/S0026261712060112
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DOI: https://doi.org/10.1134/S0026261712060112