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Effect of oxylipins on Neurospora crassa growth and differentiation

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Abstract

The effect of the natural oxylipins 3(R)-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoic acid (3-HETE) and 18-hydroxy-(9Z,12Z)-octadecadienoic acids (18-HODE) on the growth and hypha aggregation, as well as on some light-depending processes, such as carotenoid biosynthesis, protoperithecia formation (sexual cycle), and conidiation (asexual cycle), of the ascomycete Neurospora crassa was studied. Hypha aggregation and growth slowdown were induced by 3-HETE, 18-HODE, and linoleic acid. At concentrations from 5 to 50 μM, these compounds had no significant effect on the light-induced carotenogenesis. At the same time, these 3-HETE and 18-HODE concentrations, unlike linoleic acid, induced the formation of protoperithecia in the dark. At the concentration of 5 μM, an additive effect of oxylipins and light was revealed. The studied oxylipins had different effects on the asexual reproduction of N. crassa: 3-HETE induced conidiation in the dark, whereas 18-HODE induced conidiation in the light. The possible involvement of oxylipins in the regulation of the processes of sexual and asexual reproduction of N. crassa is discussed.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia

    N. N. Gessler, S. Yu. Filippovich, G. P. Bachurina, E. A. Dorodnikova & T. A. Belozerskaya

  2. Faculty of Biotechnology and Organic Synthesis, Lomonosov Moscow State Academy of Fine Chemical Technology, Moscow, Russia

    N. V. Groza

  3. Faculty of Biology, Moscow State University, Moscow, Russia

    T. A. Belozerskaya

Authors
  1. N. N. Gessler
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  2. S. Yu. Filippovich
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  3. G. P. Bachurina
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  4. N. V. Groza
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  5. E. A. Dorodnikova
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  6. T. A. Belozerskaya
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Corresponding author

Correspondence to T. A. Belozerskaya.

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Original Russian Text © N.N. Gessler, S.Yu. Filippovich, G.P. Bachurina, N.V. Groza, E.A. Dorodnikova, T.A. Belozerskaya, 2012, published in Mikrobiologiya, 2012, Vol. 81, No. 5, pp. 587–593.

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Gessler, N.N., Filippovich, S.Y., Bachurina, G.P. et al. Effect of oxylipins on Neurospora crassa growth and differentiation. Microbiology 81, 542–548 (2012). https://doi.org/10.1134/S0026261712050074

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  • DOI: https://doi.org/10.1134/S0026261712050074

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