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Induction of programmed lysis in Streptomyces lividans culture by the inhibitors of eukaryotic type serine/threonine protein kinases

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Abstract

Programmed death (PD) of the mycelium of Streptomyces lividans, namely, its delayed lysis in response to treatment with indolylmaleimide derivatives, which inhibit actinobacterial serine/threonine protein kinases (STPK), is described. Delayed lysis of mycelial cell was accompanied by DNA damage similar to PD in differentiating S. lividans mycelium. Two-dimensional electrophoresis and mass spectrometry were used to identify proteins up-regulated by a PD-inducing STPK inhibitor. Most of these proteins are known to be implicated in responses to various stress stimuli. Thus, our model of delayed cell lysis of actinobacteria upon STPK inhibition may serve for unveiling the molecular mechanisms of bacterial PD and for antimicrobial drug design.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia

    O. B. Bekker, D. A. Mavletova, I. K. Lyubimova, T. A. Mironcheva, A. A. Shtil’ & V. N. Danilenko

  2. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia

    A. A. Shtil’

Authors
  1. O. B. Bekker
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  2. D. A. Mavletova
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  3. I. K. Lyubimova
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  4. T. A. Mironcheva
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  5. A. A. Shtil’
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  6. V. N. Danilenko
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Correspondence to O. B. Bekker.

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Original Russian Text © O.B. Bekker, D.A. Mavletova, I.K. Lyubimova, T.A. Mironcheva, A.A. Shtil’, V.N. Danilenko, 2012, published in Mikrobiologiya, 2012, Vol. 81, No. 2, pp. 177–184.

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Bekker, O.B., Mavletova, D.A., Lyubimova, I.K. et al. Induction of programmed lysis in Streptomyces lividans culture by the inhibitors of eukaryotic type serine/threonine protein kinases. Microbiology 81, 160–167 (2012). https://doi.org/10.1134/S0026261712020038

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