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Obtaining and characterization of “Holospora curviuscula” and Holospora obtusa, bacterial symbionts of the macronuclei of Paramecium bursaria and Paramecium caudatum

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Abstract

The symbionts of the macronuclei of Paramecium bursaria and P. caudatum, “Holospora curviuscula” 02AZ16 and H. obtusa 88Ti, respectively, were obtained and investigated. The 16S rDNA nucleotide sequences of “Holospora curviuscula” were obtained for the first time. The differences in 16S rDNA (3.4%) suggest their classification within the genus Holospora. Molecular phylogenetic analysis of the symbionts revealed that these intranuclear symbionts of the ciliates belonged to the order Rickettsiales, forming within a compact cluster of related species.

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Authors and Affiliations

  1. Department of Invertebrate Zoology, Faculty of Biology and Soil Science, St. Petersburg State University, St. Petersburg, Russia

    N. D. Vakkerov-Kouzova & M. S. Rautian

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  1. N. D. Vakkerov-Kouzova
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  2. M. S. Rautian
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Correspondence to N. D. Vakkerov-Kouzova.

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Original Russian Text © N.D. Vakkerov-Kouzova, M.S. Rautian, 2011, published in Mikrobiologiya, 2011, Vol. 80, No. 5, pp. 714–718.

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Vakkerov-Kouzova, N.D., Rautian, M.S. Obtaining and characterization of “Holospora curviuscula” and Holospora obtusa, bacterial symbionts of the macronuclei of Paramecium bursaria and Paramecium caudatum . Microbiology 80, 728–732 (2011). https://doi.org/10.1134/S0026261711050171

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