Skip to main content
SpringerLink
Log in

Major factors affecting isocitrate lyase activity in Rhodobacter capsulatus B10 under phototrophic conditions

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

External factors affecting the activity of isocitrate lyase (ICL) in Rhodobacter capsulatus B10 grown under controlled photoheterotrophic anaerobic conditions were investigated. The activity of this enzyme was found to depend on the history of the inoculum and on the growth phase on acetate medium. Intracellular degradation of ICL under unfavorable conditions was shown. However, transition of the growing culture from acetate to lactate did not result in active degradation of the enzyme. When transferred to acetate, Rba. capsulatus could grow without the lag phase and did not exhibit ICL activity, suggesting another anaplerotic pathway in Rba. capsulatus cells. Since emergence of the ICL activity in the cells grown on acetate results in an increase in its growth rate, the glyoxylate bypass plays an important role in acetate metabolism of Rba. Capsulatus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Albers, H. and Gottschalk, G., Acetate Metabolism in Rhodopseudomonas gelatinosa and Several Other Rhodospirillaceae, Arch. Microbiol., 1976, vol. 111, nos. 1–2, pp. 45–49.

    Article  PubMed  CAS  Google Scholar 

  2. Meister, M., Saum, S., Alber, B.E., and Fuchs, G., L-Malyl-Coenzyme A/β-Methylmalyl-Coenzyme A Lyase Is Involved in Acetate Assimilation of the Isocitrate Lyase-Negative Bacterium Rhodobacter capsulatus, J. Bacteriol., 2005, vol. 187, no. 4, pp. 1415–1425.

    Article  PubMed  CAS  Google Scholar 

  3. Kornberg, H.L. and Lascelles, J., The Formation of Isocitratase by the Athiorhodaceae, J. Gen. Microbiol., 1960, vol. 23, pp. 511–517.

    PubMed  CAS  Google Scholar 

  4. Nielsen, A.M., Rampsch, B.J., and Sojka, G.A., Regulation of Isocitrate Lyase in a Mutant of Rhodopseudomonas capsulatus, Arch. Microbiol., 1979, vol. 120, no. 1, pp. 43–46.

    Article  CAS  Google Scholar 

  5. Willison, J.C., Pyruvate and Acetate Metabolism in the Photosynthetic Bacterium Rhodobacter capsulatus, J. Gen. Microbiol., 1988, vol. 134, pp. 2429–2439.

    CAS  Google Scholar 

  6. Blasco, R., Cardenas, J., and Castillo, F., Regulation of Isocitrate Lyase in Rhodobacter capsulatus E1F1, Curr. Microbiol., 1991, vol. 22, no. 1, pp. 73–76.

    Article  CAS  Google Scholar 

  7. Lavrinenko, E.P., Determination of Isocitrate Lyase in the Purple Nonsulfur Bacterium Rhodobacter capsulatus B10, in Biologiya-nauka XXI veka (Biology, The Science of the 21st Century), 12th Int. Pushchino School-Conference for Young Scientists, 2007.

  8. Ormerod, J.G., Ormerod, S.K., and Gest, H., Light-Dependent Utilization of Organic Compounds and Photoproduction of Hydrogen by Photosynthetic Bacteria: Relationship with Metabolism, Arch. Biochem. Biophys., 1961, vol. 64, pp. 449–463.

    Article  Google Scholar 

  9. Tsygankov, A.A., Laurinavichene, T.V., and Gogotov, I.N., Laboratory Scale Photobioreactor, Biotechnol. Tech., 1994, vol. 8, pp. 575–578.

    Article  CAS  Google Scholar 

  10. Dixon, G.H. and Kornberg, H.L., Assay Methods for Key Enzymes of Glyoxylate Cycle, Biochem. J., 1959, vol. 72, no. 1, p. 3.

    Google Scholar 

  11. Lowry, O.H., Rosenbrough, W.J., Farr, A.L., and Randall, R.J., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1963, vol. 193, pp. 265–275.

    Google Scholar 

  12. Manual of Methods for General Bacteriology, Gerhardt, P., Murray, R.G.E., Costilow, R.N., Nester, E.W., Wood, W.A., Krieg, N.R., and Phillips, G.B., Eds., Washington: Amer. Soc. Microbiol., 1981 [Russ. Transl. Moscow: Mir, 1984].

    Google Scholar 

  13. Laurinavichene, T.V., Tekucheva, D.N., Laurinavichius, K.S., Ghirardi, M.L., Seibert, M., and Tsygankov, A.A., Towards the Integration of Dark and Photo Fermentative Waste Treatment. 1. Hydrogen Photoproduction by Purple Bacterium Rhodobacter capsulatus Using Potential Products of Starch Fermentation, Int. J. Hydrogen Energy, 2008, vol. 33, pp. 7020–7026.

    Article  CAS  Google Scholar 

  14. Asatiani, V.S., Fermentnye metody analiza, (Enzymatic Methods of Analysis), Moscow: Nauka, 1969.

    Google Scholar 

  15. Clayton, R.K., Spectroscopic Analysis of Bacteriochlorophylls in vitro and in vivo, Photochem. Photobiol., 1966, vol. 5, pp. 669–677.

    Article  CAS  Google Scholar 

  16. Kondrat’eva, E.N., Avtotrofnye prokarioty, (Autotrophic Prokaryotes), Moscow: Mosk. Gos. Univ., 1996.

    Google Scholar 

  17. Conrad, R., and Schlegel, H.G., Different Degradation Pathways for Glucose and Fructose in Rhodopseudomonas capsulate, Arch. Microbiol, 1977, vol. 112, pp. 39–48.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    E. P. Petushkova & A. A. Tsygankov

Authors
  1. E. P. Petushkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Tsygankov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. P. Petushkova.

Additional information

Original Russian Text © E.P. Petushkova, A.A. Tsygankov, 2011, published in Mikrobiologiya, 2011, Vol. 80, No. 5, pp. 606–611.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Petushkova, E.P., Tsygankov, A.A. Major factors affecting isocitrate lyase activity in Rhodobacter capsulatus B10 under phototrophic conditions. Microbiology 80, 619–623 (2011). https://doi.org/10.1134/S0026261711050092

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261711050092

Keywords

Search

Navigation