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The effect of pyrogenically modified substrates on mineralizing activity and growth strategies of microorganisms of grey forest soil

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Abstract

The rates of the mineralization processes initiated by the input of plant residues and pyrogenically modified plant material into gray forest soil under forests and meadows were assayed. While meadow plant residues was mineralized more rapidly than the forest floor, decomposition of the pyrogenic material resulted in disproportional changes in CO2 emission from soils. Statistical treatment showed that the respiratory activity of CO2 emission by heterotrophic microorganisms, which is a physiological characteristic of microbial communities, is 89% determined by the substrate quality. The maximal specific growth rate, which reflects the functional changes in microbial communities, was affected by the cenosis (36%) and the substrate (30%). Most of the carbon of the original plant material (up to 90%) was removed during the burning of plant substrates. The remaining compounds in the pyrogenically transformed material changed the process of mineralization in soil compared both to the control variant and to soil enriched with plant residues. Input of plant residues and ash into the soil resulted in increased total and active biomass, while the maximal specific growth rate decreased and the generation time for the active biomass increased. In the case of soils with plant residues, these changes in the state of microbial communities were brief and occurred during the period of intense mineralization (0–5 days), while, in soils with plant ash, stable changes were revealed after more prolonged incubation. Experimental determination of the microbial biomass turnover time (MTT) by means of two methods (from the ratio between the microbial biomass and respiration and from microbial specific growth rates) made it possible to determine the economical coefficient Y for microbial communities metabolizing the substrates of different availability. Depending on the experimental variant, the Y values varied from 0.22 to 0.51. Decreased maximal specific growth rate and increased values of Y (the coefficient of efficiency of substrate utilization) showed the predominant contribution of K-strategists in the mineralization of low available substrates in soil. The balance calculations and physiological characteristics of the microbial community suggested that the priming effect was most probable in soils enriched with plant ash.

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References

  1. Marfenina, T.A., Mikrobiologicheskie aspekty okhrany pochv (Microbiological Aspects of Soil Preservation), Moscow: Nauka, 1999.

    Google Scholar 

  2. Bogorodskaya, A.V., Baranchikov, Yu.N., and Ivanova, G.A., The State of Microbial Complexes in Soils of Forest Ecosystems after Fires and Defoliation of Stands by Gypsy Moths, Pochvovedenie, 2009, no. 3, pp. 337–345 [Eur. Soil Sci. (Engl. Transl.), no. 3, pp. 310–317].

  3. Sorokin, N.D., Microbiological Monitoring of Siberian Forest Ecosystems under Different Anthropogenic Impacts, Usp. Sovrem. Biol., 1993, vol. 113, no. 2, pp. 131–140.

    Google Scholar 

  4. Blagodatskaya, E., Blagodatsky, S., Anderson, T.H., and Kuzyakov, Y., Contrasting Effects of Glucose, Living Roots and Maize Straw on Microbial Growth Kinetics and Substrate Availability in Soil, Eur. J. Soil Sci., 2009, vol. 60, pp. 186–197.

    Article  CAS  Google Scholar 

  5. Fontaine, S., Barot, M., Barrac, P., Bdioui, N., Bruno, M., and Rumpel, C., Stability of Organic Carbon in Deep Soil Layers Controlled by Fresh Carbon Supply, Nature, 2007, vol. 450, pp. 277–280.

    Article  PubMed  CAS  Google Scholar 

  6. Kniker, H., Vegetation Fires and Burnings, How Does Char Input Affect the Nature and Stability of Soil Organic Nitrogen and Carbon? Review, Biogeochemistry, 2007, vol. 85, pp. 91–118.

    Article  Google Scholar 

  7. Hilsher, A., Heisher, K., Siewert, C., and Knicker, H., Mineralization and Structural Changes during the Initial Phase of Microbial Degradation of Pyrogenic Plant Residues in Soil, Org. Geochem., 2009, vol. 40, pp. 332–342.

    Article  Google Scholar 

  8. Kuzyakov, Y., Friedel, J.K., and Stahr, K., Review of Mechanisms and Quantification of Priming Effects, Soil Biol. Biochem., 2000, vol. 32, pp. 1485–1498.

    Article  CAS  Google Scholar 

  9. Blagodatskaya, E.V., Blagodatsky, S.A., Anderson, T.H., and Kuzyakov, Y., Priming Effects in Chernozem Induced by Glucose and N in Relation To Microbial Growth Strategies, Appl. Soil. Ecol., 2007, vol. 37, pp. 95–105.

    Article  Google Scholar 

  10. Kuzyakov, Y. and Bol, R., Sources and Mechanisms of Priming Effect Induced in Two Grassland Soil Amended with Slurry and Sugar, Soil Biol. Biochem., 2006, vol. 32, pp. 747–758.

    Article  Google Scholar 

  11. Blagodatskaya, E.V., Blagodatsky, S.A., Dorodnikov, M., and Kuzyakov, Y., Elevated Atmospheric CO2 Increases Microbial Growth Rates in Soil: Results of Three CO2 Enrichment Experiments, Global Change Biol., 2009, vol. 16, no. 2, pp. 836–848.

    Article  Google Scholar 

  12. Blagodatskii, S.A., Blagodatskaya, E.V., and Rozanova, L.N., Kinetics and Growth Strategies of Microorganisms in Chernozem Soil after Prolonged Application of Different Fertilizer Systems, Mikrobiologiya, 1994, vol. 63, no. 2, pp. 298–307.

    Google Scholar 

  13. Blagodatskaya, E.V., Bogomolova, I.N., and Blagodatskii, S.A., Changes in Ecological Strategy of Soil Microbial Community upon Glucose Application, Pochvovedenie, 2001, no. 5, pp. 600–608 [Eur. Soil Sci. (Engl. Transl.), no. 5, pp. 530–537].

  14. Ermolaev, A.M. and Shirshova, L.T., Influence of Weather Conditions and Management of a Sown Meadow on the Herbage Productivity and Properties of Gray Forest Soils, Pochvovedenie, 2000, no. 12, pp. 1501–1508 [Eur. Soil Sci. (Engl. Transl.), no. 12, pp. 1321–1328].

  15. Panikov, N.S., Blagodatsky, S.A., Blagodatskaya, J.V., and Glagolev, M.V., Determination of Microbial Mineralization Activity in Soil by Modified Right and Hobby Method, Biol. Fertil. Soil, 1992, vol. 14, pp. 280–287.

    Article  CAS  Google Scholar 

  16. Panikov, N.S. and Sizova, M.V., A Kinetic Method for Estimating the Biomass of Microbial Functional Groups in Soil, J. Microbiol. Meth., 1996, vol. 24, pp. 219–230.

    Article  Google Scholar 

  17. Anderson, J.P.E. and Domsch, K.H., A Physiological Method for the Quantitative Measurement of Microbial Biomass in Soils, Soil Biol. Biochem., 1978, vol. 10, no. 3, pp. 215–221.

    Article  CAS  Google Scholar 

  18. Blagodatsky, S.A., Heinemeyer, O., and Richter, J., Estimating the Active and Total Soil Microbial Biomass by Kinetic Respiration Analysis, Biol. Fertil. Soils, 2000, vol. 32, no. 1, pp. 73–81.

    Article  CAS  Google Scholar 

  19. Cheng, W., Johnson, D.W., and Fu, S., Rhizosphere Effects on Decomposition: Controls of Plant Species, Phenology, and Fertilization, Soil Sci. Soc. Am. J., 2003, vol. 67, pp. 1418–1427.

    Article  CAS  Google Scholar 

  20. Kaal, J. and Rumpel, C., Can Pyrolysis-GC/MS Be Used to Estimate the Degree of Thermal Alternation of Black Carbon?, Org. Geochem., 2009, vol. 36, pp. 1021–1030.

    Google Scholar 

  21. Blagodatskaya, E.V., Pampura, T.V., Myakshina, T.N., and Dem’yanova, E.G., The Influence of Lead on the Respiration and Biomass of Microorganisms in Gray Forest Soil in a Long-Term Field Experiment, Pochvovedenie, 2006, no. 5, pp. 559–568 [Eur. Soil Sci. (Engl. Transl.), no. 5, pp. 498–506].

  22. Schimel, J.P. and Weintraub, M.N., The Implications of Exoenzyme Activity on Microbial Carbon and Nitrogen Limitation in Soil: a Theoretical Model, Soil Biol. Biochem., 2003, vol. 35, pp. 549–563.

    Article  CAS  Google Scholar 

  23. Rumpel, C., Gonzales-Perez, J.A., Bardoux, J., Largeau, C., Gonzales-Vila, F.J., and Valentin, C., Composition and Reactivity of Morphologically Distinct Charred Materials Left after Slash and Burn Practices in Agricultural Tropical Soils, Org. Geochem., 2007, vol. 38, pp. 911–920.

    Article  CAS  Google Scholar 

  24. Fernandez, I., Cabaniero, A., and Carballas, T., Carbon Mineralization Dynamics in Soils after Wildfires in Two Galician Forests, Soil Biol. Biochem., 1999, vol. 31, pp. 1853–1865.

    Article  CAS  Google Scholar 

  25. Kniker, H., Gonzales-Vila, F.J., Martin, F., and Lüdemann, H.-D., Fire-Induced Transformation of C- and N-Forms in Different Organic Soil Fractions from a Dystric Cambisoil under a Mediterranean Pine Forest, Soil Biol. Biochem., 2005, vol. 37, pp. 701–718.

    Article  Google Scholar 

  26. Blagodatskaya, E.V. and Kuzyakov, Y., Mechanisms of Real and Apparent Priming Effects and Their Dependence on Soil Microbial Biomass and Community Structure: Critical Review, Biol. Fertil. Soil, 2008, vol. 45, pp. 115–131.

    Article  Google Scholar 

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Authors and Affiliations

  1. Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    A. I. Zhuravleva, T. N. Myakshina & E. V. Blagodatskaya

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  1. A. I. Zhuravleva
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  2. T. N. Myakshina
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  3. E. V. Blagodatskaya
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Correspondence to A. I. Zhuravleva.

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Original Russian Text © A.I. Zhuravleva, T.N. Myakshina, E.V. Blagodatskaya, 2011, published in Mikrobiologiya, 2011, Vol. 80, No. 2, pp. 207–218.

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Zhuravleva, A.I., Myakshina, T.N. & Blagodatskaya, E.V. The effect of pyrogenically modified substrates on mineralizing activity and growth strategies of microorganisms of grey forest soil. Microbiology 80, 194–204 (2011). https://doi.org/10.1134/S0026261711020202

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