Abstract
The structure and immunochemical properties of lipopolysaccharide from Budvicia aquatica 97U124, a representative of a novel species of Enterobacteriaceae, were studied. The O-polysaccharide (OPS) was isolated by mild acid hydrolysis and characterized by monosaccharide analysis and NMR spectroscopy. It was shown that the OPS from B. aquatica 97U124 consisted of repeating units with the structure
Similar content being viewed by others
References
Aldova, E., Hausner, O., and Gabrhelova, M., A Hydrogen Sulfide Producing Gram-Negative Rod from Water, Zbl. Bakteriol. Parasistenkd. Infektionskr. Hyd. Abt. 1 Orig. Reihe A, 1983, no. 254, pp. 95–108.
Bouvet, O., Grimont, P., and Richard, C., Budvicia aquatica gen. nov.: a Hydrogen Sulfide-Producing Member of the Enterobacteriaceae, Int. J. Syst. Bacteriol., 1995, vol. 3, pp. 208–218.
Blokhina, I.N., Sokolova, K.Ya., and Levanova, G.F., News in Classification and Identification of Enterobacteria, Zh. Mikrobiol. Epidemiol. Immunobiol., 1992, no. 1, pp. 49–52.
Pokhil, S.I., Sites of Activity of Bacteria Budvicia aquatica in Ukraine. Biological Properties of B. aquatica Strains Isolated in Ukraine and Czech Republic, Annaly Mechnikovskogo Instituta, 2002, no. 2–3, pp. 20–26.
Vidaver, A., Synthetic and Complex Media for the Rapid Detection of Fluorescence and Phytopathogenic Pseudomonas: Effect of Carbon Source, Appl. Microbiol., 1967, vol. 15, no. 6, pp. 1523–1524.
Westphal, O and Jann, K, Bacterial Lipopolysaccharides extraction with Phenol-Water and Further Application of the Procedure, in Methods in Carbohydrate Chemistry, Whistler, R.L. and Wolfrom, M.L., Eds., New York: Academic, 1965, pp. 83–91.
Dubois, M., Gilles, K., Hamilton, J., Rebers, P.A., and Smith, F., Colorimetric Method for Determinationn of Sugars and Related Substrances, Anal. Chem., 1956, vol. 28, no. 2, pp. 350–356.
Lowry, O., Rosenbrough, N., Farr, A., and Randall, R., Protein Measurement with the Folin Reagent, J. Biol. Chem., 1951, vol. 193, no. 1, pp. 265–275.
Spirin, A.S., Determination of Nucleic Acids, Biokhimiya, 1958, vol. 23, no. 5, pp. 562–662.
Varbanets, L.D., Zdorovenko, G.M., and Knirel’, Yu.A., Metody Issledovaniya Endotoksinov (Methods of Endotoxin Investigation), Kiev: Naukova Dumka, 2006, p. 237.
Sawardeker, J.S., Sloneker, J.H., and Jeans, A., Quantitative Determination of Monosaccharides as Their Alditol Acetates by Gas Liquid Chromatography, Anal. Chem., 1965, vol. 37, pp. 1602–1603.
Leontein, K. and Longren, J., Determination of the Absolute Configuration of Sugars by Gas-Liquid Chromatography of Their Acetylated 2-Octyl-Glycosides, Meth. Carbohydr. Chem., 1993, vol. 9, pp. 87–89.
Ouchterlony, O., Diffusion in Gel Methods for Immunological Analysis, Prog. Allergy, 1962, no. 6, pp. 3–15.
Akiyama, Y., Nishckawaji, S., and Eda, S., Lipopolysaccharides of Pseudomonas solanacearum, Agric. Biol. Chem., 1985, vol. 49, no. 4, pp. 1193–1194.
Bock, K. and Pedersen, C., Carbon-13 Nuclear Magnetic Resonance Spectroscopy of Monosaccharides, Adv. Carbohydr. Chem. Biochem., 1983, vol. T. 41, pp. 27–66.
Naumova, I.B., Sadikov, B.M., Streshinskaya, G.M., and Polin, A.N., Teichoic Acid in Arthrobacter crystallopoietes Cell Wall, Antibiot. Med. Biotekhnol., 1987, no. 32, pp. 107–111.
Varbanets, L.D., Shashkov, A.S., and Kocharova, N.A., Phosphorus-Containing Glycopolymers of Clavibacter michiganense Cell Walls, Carbohydr. Res., 1990, vol. 204, pp. 157–160.
Streshinskaya, G.M., Tul’skaya, E.M., Shashkov, A.S., Evtushenko, L.I., Taran, V.V., and Naumova, I.B., Teichoic Acids of the Cell Wall of Nocardiopsis listeri, Nocardiopsis lucentensis, and Nocardiopsis trehalosei, Biokhimiya, 1998, vol. 63, no. 2, pp. 230–234 [Biochemistry (Moscow) (Engl. Transl.), vol. 63, no. 2, pp. 191–194.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.L. Zdorovenko, L.D. Varbanets, O.S. Brovarskaya, O.A. Valueva, A.S. Shashkov, Yu.A. Knirel’, 2011, published in Mikrobiologiya, 2011, Vol. 80, No. 3, pp. 366–371.
Rights and permissions
About this article
Cite this article
Zdorovenko, E.L., Varbanets, L.D., Brovarskaya, O.S. et al. Lipopolysaccharide of Budvicia aquatica 97U124: Immunochemical properties and structure. Microbiology 80, 372–377 (2011). https://doi.org/10.1134/S0026261711020196
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026261711020196