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Genome variability of the yeast Yarrowia lipolytica

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Abstract

Using sequence analysis of internal transcribed spacers ITS1 and ITS2, RAPD-PCR, and pulsed-field gel electrophoresis of intact chromosomal DNA, we investigated the molecular and genetic peculiarities of the genomes of 40 Yarrowia lipolytica strains. All the strains showed nearly identical ITS-sequences. On the other hand, karyotypic analysis revealed significant differences in the chromosomal patterns of Y. lipolytica. The number and order of individual chromosomes vary from strain to strain. Chromosome-length polymorphism of the Y. lipopytica strains was pronounced and independent of their geographic origin and the source of isolation. Intraspecific polymorphism of Y. lipolytica chromosomes is discussed.

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References

  1. Barth, G. and Gaillardin, C., Physiology and Genetics of the Dimorphic Fungus Yarrowia lipolytica, FEMS Microbiol. Rev., 1997, vol. 19, pp. 219–237.

    Article  CAS  PubMed  Google Scholar 

  2. Kurtzman, C.P., New Species and a New Combination in the Hyphopichia and Yarrowia Yeast Clades, Antonie van Leeuwenhoek J. Microbiol. Serol., 2005, vol. 88, pp. 121–130.

    Article  CAS  Google Scholar 

  3. Scannell, D.R., Frank, A.C., Conant, G.C., Byrne, K.P., Woolfit, M., and Wolfe, K.H., Independent Sorting-Out of Thousands of Duplicated Gene Pairs in Two Yeast Species Descended from a Whole-Genome Duplication, Proc. Nat. Acad. Sci. USA, 2007, vol. 104, pp. 8397–8402.

    Article  CAS  PubMed  Google Scholar 

  4. Kurtzman, C.P. and Robnett, C.J., Identification and Phylogeny of Ascomycetous Yeasts from Analysis of Nuclear Large Subunit (25S) Ribosomal DNA Partial Sequences, Antonie van Leeuwenhoek J. Microbiol. Serol., 1998, vol. 73, pp. 331–371.

    Article  CAS  Google Scholar 

  5. Kurtzman, C.P. and Robnett, C.J., Phylogenetic Relationships among Yeasts of the “Saccharomyces Complex” Determined from Multigene Sequence Analyses, FEMS Yeast Res., 2003, vol. 3, pp. 417–432.

    Article  CAS  PubMed  Google Scholar 

  6. Valente, P., Ramos, J.P., and Leoncini, O., Sequencing as a Tool in Yeast Molecular Taxonomy, Can. J. Microbiol., 1999, vol. 45, pp. 949–958.

    Article  CAS  PubMed  Google Scholar 

  7. James, S.A., Roberts, I.N., and Collins, M.D., Phylogenetic Heterogeneity of the Genus Williopsis as Revealed by 18S rRNA Gene Sequences, Int. J. Syst. Bacteriol., 1998, vol. 48, pp. 591–596.

    Article  CAS  PubMed  Google Scholar 

  8. James, S.A., Collins, M.D., and Roberts, I.N., Use of an rRNA Internal Transcribed Spacer Region to Distinguish Phylogenetically Closely Related Species of the Genera Zygosaccharomyces and Torulaspora, Int. J. Syst. Bacteriol., 1996, vol. 46, no. 1, pp. 189–194.

    Article  CAS  PubMed  Google Scholar 

  9. Naumov, G.I., Naumova, E.S., Lantto, R.A., Louis, E.J., and Korhola, M., Genetic Homology Between Saccharomyces cerevisiae and Its Sibling Species S. paradoxus and S. bayanus: Electrophoretic Karyotypes, Yeast, 1992, vol. 8, pp. 599–612.

    Article  CAS  PubMed  Google Scholar 

  10. Boekhout, T. and Naumova, E.S., Pulsed Field Gel Electrophoresis (PFGE) of Yeasts, in Experimental Approaches for Assessing Genetic Diversity among Microbial Pathogens, van Belkum, A. et al., Eds., Wageningen, The Netherlands, 2003, pp. 93–103.

  11. Kumar, S., Tamura, K., and Nei, M., MEGA3: Integrated Software for Molecular Evolutionary Genetics Analysis and Sequence Alignment, Briefings in Bioinformatics, 2004, vol. 5, pp. 150–163.

    Article  CAS  PubMed  Google Scholar 

  12. Naumova, E.S., Zholudeva, M.V., Martynenko, N.N., and Naumov, G.I., The Molecular Genetic Differentiation of Cultured Saccharomyces Strains, Mikrobiologiya, 2005, vol. 74, no. 2, pp. 179–187 [Microbiology (Engl. Transl.), vol. 74, no. 2, pp. 179–187].

    CAS  Google Scholar 

  13. Casarégola, S., Feynerol, C., Diez, M., Fournier, P., and Gaillardin, C., Genomic Organization of the Yeast Yarrowia lipolytica, Chromosoma, 1997, vol. 106, pp. 380–390.

    Article  PubMed  Google Scholar 

  14. Knutsen, A.K., Robert, V., Poot, G.A., Epping, W., Figge, M., Holst-Jensen, A., Skaar, I., and Smith, M.T., Polyphasic Re-Examination of Yarrowia lipolytica Strains and the Description of Three Novel Candida Species: Candida oslonensis sp. nov., Candida alimentaria sp. nov. and Candida hollandica sp. nov., Int. J. Syst. Evol. Microbiol., 2007, vol. 57, pp. 2426–2435.

    Article  CAS  PubMed  Google Scholar 

  15. Naumova, E., Naumov, G., Fournier, P., Nguyen, H.V., and Gaillardin, C., Chromosomal Polymorphism of the Yeast Yarrowia lipolytica and Related Species: Electrophoretic Karyotyping and Hybridization with Cloned Genes, Curr. Genet., 1993, vol. 23, pp. 450–454.

    Article  CAS  PubMed  Google Scholar 

  16. Nga, B.N., Yip, C.W., Koh, S.I., and Chiu, L.L., Variation of the Electrophoretic Karyotypes in Genetically Different Strains of Saccharomycopsis fibuligera and Yarrowia lipolytica, Microbiology, 1995, vol. 141, pp. 705–711.

    Article  CAS  Google Scholar 

  17. Dujon, B., Yeasts Illustrate the Molecular Mechanisms of Eukaryotic Genome Evolution, Trends Genet., 2006, vol. 7, pp. 375–387.

    Article  Google Scholar 

  18. Gaillardin, C.M., Charoy, V., and Heslot, H., A Study of Copulation, Sporulation and Meiotic Segregation in Candida lipolytica, Arch. Mikrobiol., 1973, vol. 92, pp. 69–83.

    Article  CAS  PubMed  Google Scholar 

  19. Ogrydziak, D., Bassel, J., and Mortimer, R., Development of the Genetic Map of the Yeast Saccharomycopsis lipolytica, Mol. Gen. Genet., 1982, vol. 188, pp. 179–183.

    Article  CAS  PubMed  Google Scholar 

  20. Wickerham, L.J., Kurtzman, C.P., and Herman, A.I., Sexual Reproduction in Candida lipolytica, Science, 1970, vol. 167, p. 1141.

    Article  CAS  PubMed  Google Scholar 

  21. Fickers, P., Nicaud, J.M., Destain, J., and Thonart, P., Overproduction of Lipase by Yarrowia lipolytica Mutants, Appl. Microbiol. Biotechnol., 2003, vol. 63, pp. 136–142.

    Article  CAS  PubMed  Google Scholar 

  22. Fickers, P., Fudalej, F., Le Dall, M.T., Casaregola, S., Gaillardin, C., Thonart, P., and Nicaud, J.M., Identification and Characterisation of LIP7 and LIP8 Genes Encoding Two Extracellular Triacylglycerol Lipases in the Yeast Yarrowia lipolytica, Fungal Genet. Biol., 2005, vol. 42, pp. 264–274.

    Article  CAS  PubMed  Google Scholar 

  23. Aloulou, A., Rodriguez, J.A., Puccinelli, D., Mouz, N., Leclaire, J., Leblond, Y., and Carrière, F., Purification and Biochemical Characterization of the LIP2 Lipase from Yarrowia lipolytica, Biochim. Biophys. Acta, 2007, vol. 1771, pp. 228–237.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. State Institute for Genetics and Selection of Industrial Microorganisms, 1 Dorozhnyi proezd, 1, Moscow, 117545, Russia

    E. S. Naumova, E. V. Serpova & G. I. Naumov

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  1. E. S. Naumova
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  2. E. V. Serpova
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  3. G. I. Naumov
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Correspondence to G. I. Naumov.

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Original Russian Text © E.S. Naumova, E.V. Serpova, G.I. Naumov, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 2, pp. 252–259.

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Naumova, E.S., Serpova, E.V. & Naumov, G.I. Genome variability of the yeast Yarrowia lipolytica . Microbiology 79, 229–236 (2010). https://doi.org/10.1134/S0026261710020153

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