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Isolation and characterization of a new low-molecular antibacterial peptide of the lantibiotics family

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Abstract

The physicochemical and biological properties of the low-molecular antibacterial peptide isolated from the cultivation medium of Staphylococcus warneri IEGM KL-1 were studied. The peptide was obtained in a homogenous state by the methods of ultrafiltration, ion exchange, and reversed phase chromatography. The peptide contained a substantial quantity of cationic and hydrophobic amino acid residues and an uncommon amino acid lanthionine. The molecular mass of the peptide was 2999 Da. A bactericidal effect of the isolated peptide on the cells of S. epidermidis 33 was exhibited in a wide pH range, being completely preserved upon heat treatment. In accordance with the characteristics, origin, and species affiliation of the producer, the peptide was named warnerin. The available data allow us to consider warnerin as a new representative of the family of lantibiotics, promising antibiotic agents of microbial origin.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences, ul. Goleva 13, Perm, 614081, Russia

    V. P. Korobov, L. M. Lemkina & T. V. Polyudova

  2. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia

    V. K. Akimenko

Authors
  1. V. P. Korobov
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  2. L. M. Lemkina
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  3. T. V. Polyudova
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  4. V. K. Akimenko
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Correspondence to V. P. Korobov.

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Original Russian Text © V.P. Korobov, L.M. Lemkina, T.V. Polyudova, V.K. Akimenko, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 2, pp. 228–238.

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Korobov, V.P., Lemkina, L.M., Polyudova, T.V. et al. Isolation and characterization of a new low-molecular antibacterial peptide of the lantibiotics family. Microbiology 79, 206–215 (2010). https://doi.org/10.1134/S0026261710020128

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  • DOI: https://doi.org/10.1134/S0026261710020128

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