Skip to main content
Log in

Influence of the introduction of Caragana arborescens on the composition of its root-nodule bacteria

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

The genetic diversity and phylogeny of root module bacteria entering into symbiotic relations with Caragana arborescens introduced on the territory of the Republic of Bashkortostan (RB) were studied. Analysis of the 85 strains isolated from root-nodules showed that, according to the DNA polymorphism revealed by RAPD analysis, they can be divided into 12 groups. It was found that, both in natural habitats and on the territory of the RB, Caragana arborescens is primarily nodulated by Mesorhizobium bacteria whose 16S rRNA gene sequences differ to some degree from those of the bacteria described earlier by Chinese authors. Bacteria assigned to the genus Phyllobacterium based on their 16S rRNA gene sequences were also revealed in plants growing on the territory of the RB.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Guan, S.H., Chen, W.F., Wang, E.T., Lu, Y.L., Yan, X.R., Zhang, X.X., and Chen, W.X., Mesorhizobium caraganae sp. nov., a Novel Rhizobial Species Nodulated with Caragana spp. in China, Int. J. Syst. Evol. Microbiol., 2008, vol. 58, pp. 2646–2653.

    Article  CAS  PubMed  Google Scholar 

  2. Yan, X.R., Chen, W.F., Fu, J.F., Lu, Y.L., Xue, C.Y., Sui, X.H., Li, Y., Wang, E.T., and Chen, W.X., Mesorhizobium spp. Are the Main Microsymbionts of Caragana spp. Grown in Liaoning Province of China, FEMS Microbiol. Lett., 2007, vol. 271, no. 2, pp. 265–273.

    Article  CAS  PubMed  Google Scholar 

  3. Jarvis, B.D.W., van Berkum, P., Chen, W.X., Nour, S.M., Fernandez, M.P., Cleyet-Marel, J.C., and Gillis, M., Transfer of Rhizobium loti, Rhizobium hua-MICROBIOLOGY Vol. 79 No. 1 2010 kii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshansense to Mesorhizobium gen. nov., Int. J. Syst. Bacteriol., 1997, vol. 47, pp. 895–898.

    Article  Google Scholar 

  4. Van Berkum, P. and Eardly, B.D, Molecular Evolutionary Systematics of the Rhizobiaceae, in The Rhizobiaceae, Spaink, H.P., Kondorosi, A., and Hooykaas, P.J.J., Eds., Dordrecht: Kluwer, 1998, pp. 1–24.

    Google Scholar 

  5. Chen, W.X., Wang, E.T., and Kuykendall, L.D., Genus Mesorhizobium, in Bergey’s Manual of Systematic Bacteriology, 2nd ed., Garrity, G.M., Ed., New York: Springer, vol. 2.

  6. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., and Tingey, S.V., DNA Polymorphisms Amplified by Arbitrary Primers Are Useful as Genetic Markers, Nucleic Acid Res., 1990, vol. 25, pp. 6531–6535.

    Article  Google Scholar 

  7. Laguerre, G., Mavingui, P., Allard, M.R., Charnay, M.P., Louvrier, P., Mazurier, S.I., Rigottier-Gois, L., and Amarger, N., Typing of Rhizobia by PCR DNA Fingerprinting and PCR-Restriction Fragment Length Polymorphism Analysis of Chromosomal and Symbiotic Gene Regions: Application to Rhizobium leguminosarum and Its Different Biovars, Appl Environ Microbiol., 1996, vol. 62, pp. 2029–2036.

    CAS  PubMed  Google Scholar 

  8. Pariiskaya, A.N. and Klevenskaya, I.L., Natural Occurrences and Possible Evolutionary Pathways of Nitro-gen-Fixin Symbiosis, Uspekhi Mikrobiol., 1979, vol. 14, pp. 124–147.

    CAS  Google Scholar 

  9. Kaneko, T., Nakamura, Y., Sato, S., Asamizu, E., Kato, T., Sasamoto, S., Watanabe, A., Idesawa, K., Ishikawa, A., Kawashima, K., Kimura, T., Kishida, Y., Kiyokawa, C, Kohara, M., Matsumoto, M., Matsuno, A., Mochizuki, Y., Nakayama, S., Nakazaki, N., Shimpo, S., Sugimoto, M., Takeuchi, C., Yamada, M., and Tabata, S., Complete Genome Structure of the Nitrogen-Fixing Symbiotic Bacterium Mesorhizobium, DNA Res., 2000, vol. 7, pp. 331–338.

    Article  CAS  PubMed  Google Scholar 

  10. Martinez, E., Romero, D., and Palacios, R., The Rhizobium Genome, Crit. Rev. Plant Sci., 1990, vol. 9, pp. 59–93.

    Article  CAS  Google Scholar 

  11. Sullivan, J.T., Patrick, H.N., Lowther, W.L., Scott, D.B., and Ronson, C.W., Nodulating Strains of Rhizobium loti Arise through Chromosomal Symbiotic Gene Transfer in the Environment, Proc. Natl. Acad. Sci. USA, 1995, vol. 92, pp. 8985–8989.

    Article  CAS  PubMed  Google Scholar 

  12. Sullivan, J.T. and Ronson, C.W., Evolution of Rhizobia by Acquisition of a 500-kb Symbiosis Island That Integrates into a phe-tRNA Gene, Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 5145–5149.

    Article  CAS  PubMed  Google Scholar 

  13. Sullivan, J.T., Trzebiatowski, J.R., Cruickshank, R.W., Gouzy, J., Brown, S.D., Elliot, R.M., Fleetwood, D.J., McCallum, N.G., Rossbach, U., Stuart, G.S., Weaver, J.E., Webby, R.J., de Bruijn, F.J., and Ronson, C.W., Comparative Sequence Analysis of the Symbiosis Island of Mesorhizobium loti Strain R7A, J. Bacteriol., 2002, vol. 184, pp. 3086–3095.

    Article  CAS  PubMed  Google Scholar 

  14. Valverde, A., “Velazquez, E., Fernandez-Santos, F, Vizcaino, N, Rivas, R., Mateos, RE, Martinez-Molina, E., Igual, J.M., and Willems, A., Phyllobacterium trifolii sp. nov., Nodulating Trifolium and Lupinus in Spanish Soils, Int. J. Syst. Evol. Microbiol., 2005, vol. 55, pp. 1985–1989.

    Article  CAS  PubMed  Google Scholar 

  15. Mantelin, S., Saux, M.F., Zakhia, F., Bena, G., Bon-neau, S., Jeder, H., de Lajudie, E, and Cleyet-Marel, J.-C., Emended Description of the Genus Phyllobacterium and Description of Four Novel Species Associated with Plant Roots: Phyllobacterium bourgognense sp. nov., Phyllobacterium ifriqiyense sp. nov., Phyllobacterium leguminum sp. nov., and Phyllobacterium brassicacearum sp. nov, Int. J. Syst. Evol. Microbiol., 2006, vol. 56, pp. 827–839.

    Article  CAS  PubMed  Google Scholar 

  16. Baimiev, Al.Kh., Baimiev, An.Kh., Gubaidullin, I.I., Kulikova, O.L., and Chemeris, A.V., Bacteria Closely Related to Phyllobacterium trifolii According to Their 16S rRNA Gene Are Discovered in the Nodules of Hungarian Sainfoin, Genetika, 2007, vol. 43, no. 5, pp. 716–719 [Russ. J. Genet. (Engl. Transl.), vol. 43, no. 5, pp 587-590].

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to An. Kh. Baymiev.

Additional information

Original Russian Text © An.Kh. Baymiev, K.G. Ptitsyn, Al.Kh. Baimiev, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 1, pp. 123–128.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baymiev, A.K., Ptitsyn, K.G. & Baimiev, A.K. Influence of the introduction of Caragana arborescens on the composition of its root-nodule bacteria. Microbiology 79, 115–120 (2010). https://doi.org/10.1134/S0026261710010157

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261710010157

Key words

Navigation