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The search for sulfate-reducing bacteria in mat samples from the lost city hydrothermal field by molecular cloning

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The work is dedicated to searching for microorganisms of the domain Bacteria capable of dissimilatory sulfate reduction in the samples of microbial mats from a carbonate chimney in the Lost City hydro-thermal field. Cloning of 16S rRNA genes, the universal phylogenetic marker, and dsrAB, the functional marker for sulfate reduction, revealed phylotypes related to spore-forming Desulfotomaculum. No members of the Deltaproteobacteria, comprising the most numerous bacterial group with demonstrated capacity for dissimilatory sulfate reduction, were found. The phylogenetic position of 16S rRNA clones from the mats suggests that this microbial community is a unique consortium, where the energy flow is related to hydrogen of hydrothermal origin, while mass growth of primary produces results from utilization of sulfide formed by sulfate-and sulfur-reducing microorganisms.

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Authors and Affiliations

  1. Tomsk State University, Tomsk, Russia

    A. L. Gerasimchuk, O. P. Butorova & O. V. Karnachuk

  2. State Research Institute for Genetics and Selection of Industrial Microorganisms, 1st Dorozhnyi pr., 1, Moscow, 117545, Russia

    A. A. Shatalov, A. L. Novikov & A. S. Yanenko

  3. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    N. V. Pimenov

  4. Shirshov Institute of Oceanology, Russian Academy of Sciences, Moscow, Russia

    A. Yu. Lein

Authors
  1. A. L. Gerasimchuk
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  2. A. A. Shatalov
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  3. A. L. Novikov
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  4. O. P. Butorova
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  5. N. V. Pimenov
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  6. A. Yu. Lein
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  7. A. S. Yanenko
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  8. O. V. Karnachuk
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Correspondence to O. V. Karnachuk.

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Original Russian Text © A.L. Gerasimchuk, A.A. Shatalov, A.L. Novikov, O. P. Butorova, N.V. Pimenov, A.Yu. Lein, A.S. Yanenko, O.V. Karnachuk, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 1, pp. 102–113.

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Gerasimchuk, A.L., Shatalov, A.A., Novikov, A.L. et al. The search for sulfate-reducing bacteria in mat samples from the lost city hydrothermal field by molecular cloning. Microbiology 79, 96–105 (2010). https://doi.org/10.1134/S0026261710010133

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  • DOI: https://doi.org/10.1134/S0026261710010133

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