Abstract
A novel obligately aerobic heterotrophic bacterium belonging to the genus Xanthobacter, strain Z-0055, was isolated from the bacterial community of dystrophic waters formed by xylotrophic fungi grown on decaying spruce wood. The cells are small (0.4 ±0.7 fum), ovoid, grarrmegative, and nonmotile. The strain reproduces by nonuniform division. Strain Z-0055 is a moderately acidophilic microorganism and grows in a pH range of 4.8–6.8, with an optimum at pH 5.5. The temperature range for growth is 10–28°C, with an optimum at 20°C. The bacterium utilizes salts of organic acids (citrate, oxalate, succinate, and gluconate), as well as xylose and xylan as carbon and energy sources. No growth was detected at NaCl concentrations higher than 1.5 g/l. The DNA G+C base content is 63.6 mol %. According to phylogenetic analysis of the 16S rRNA gene sequences, strain Z-0055 belongs to the cluster containing X. flavus, X. aminoxidans, X. autotrophicus, and X. viscosus. The levels of similarity between the studied strain and these species are almost the same (96.0–97.0%). The genetic and ecophysiological properties of strain Z-0055 support classification of this acid-tolerant inhabitant of oligotrophic waters within the genus Xanthobacter as a new species X. xylophilus sp. nov.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Wiegel, J., The Genus Xanthobacter, Prokaryotes, 2006, vol. 5, pp. 290–314.
Garrity, G.M., Bell, J.A., and Lilburn, T., Family VIII. Hyphomicrobiaceae, in Bergey’s Manual of Systematic Bacteriology. 2nd ed., Brenner, D.J., Krieg, N.R., and Staley, J.T., Eds., New York: Springer, 2005, vol. 2, part C, pp. 476–555.
Chistyakova, I.K., C 1 Compounds-Utilizing Nitrogen-Fixing Bacteria in Soils under Rice, Mikrobiologiya, 1985, vol. 53, no. 3, pp. 384–388.
Doronina, N.V. and Trotsenko, Y.A., Reclassification of “Blastobacter viscosus” 7d and “Blastobacter ami-nooxidans” 14a as Xanthobacter aminoxidans sp. nov. and Xanthobacter viscosus sp. nov., Int. J. Syst. Evol. Microbiol., 2003, vol. 53, no. 1, pp. 179–182.
Loginova, N.V., Govorukhina, N.I., and Trotsenko, Yu.A., Autotrophic Metabolism of Methanol in Blastobacter viscosus, Mikrobiologiya, 1982, vol. 50, pp. 428–434.
Doronina, N.V., Govorukhina, N.I., and Trotsenko, Yu.A., Blastobacter aminooxidans, a New Species of Bacteria Growing Autotrophically on Methylated Amines, Mikrobiologiya, 1984, vol. 52, pp. 547–553.
Padden, A.N., Rainey, F.A., Kelly, D.P., and Wood, A.P., Xanthobacter tagetides sp. nov., an Organism Associated with Tagetes Species and Able to Grow on Substituted Thhiophenes, Int. J. System. Bacteriol., 1997, vol. 47, pp. 394–401.
Hirano, S.L., Kitauchi, F., Haruki, M., Imanaka, T., Morikava, M., and Kanaya, S., Isolation and Characterization of Xantobacter polyaromaticivorans sp. nov. 127 W That Degrades Polycyclic and Heterocyclic Aromatic Compounds under Extremely Low Oxygen Conditions, Boisci. Biotechnol. Biochem., 2004, vol. 68, pp. 557–564.
Zavarzin, G.A. and Zavarzina, A.G., Xylotrophic and Mycophilic Bacteria in Formation of Dystrophic Waters, Mikrobiologiya, 2009, vol. 78, no. 5, pp. 579–591 [Microbiology (Engl. Transl.), vol. 78, no. 5, pp. 523-534].
Cohen-Bazire, G., Sistrom, W.R., and Stanier, RY, Kinetic Studies of Pigment Synthesis by Non-Sulphur Purple Bacteria, J. Cell. Comp. Physiol., 1957, vol. 49, pp. 25–68.
Marusina, A.I., Bulygina, E.S., Kuznetsov, B.B., Tourova, T.P., Kravchenko, I.K., and Gal’chenko, V.F., A System of Oligonucleotide Primers for the Amplification of nifH Genes of Different Taxonomic Groups of Prokaryotes, Mikrobiologiya, 2001, vol. 70, no. 1, pp. 86–91 [Microbiology (Engl. Transl.), vol. 70, no. 1, pp. 73-78].
Poly, F., Monrozier, L.J., and Bally, R., Improvement in the RFLP Procedure for Studying the Diversity of NifH Genes in Communities of Nitrogen Fixers in Soil, Res. Microbiol., 2001, vol. 152, pp. 95–103.
Davis, B.H., Analysis of Carotenoid Pigments, in Chemistry and Biochemistry of Plant Pigment, Goodwin, T.W, Ed., London: Acad. Press, 1965.
Lysenko, A.M., Gal’chenko, V.F., and Chernykh, A.N., Taxonomic Investigation of Obligate Methanotrophic Bacteria by DNA/DNA Hybridization, Mikrobiologiya, 1988, vol. 57, no. 5, pp. 816–822.
Marmur, J., A Procedure for Isolation of DNA from Microorganisms, J. Mol. Biol., 1961, vol. 3, pp. 208–214.
Van de Peer, Y. and De Watcher, R., Treecon for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Comput. Applic. Biosci., 1994, vol. 10, pp. 569–570.
Lee, K.B., Liu, C.T., Anzai, Y., Kim, H., Aono, T., and Oyaizu, H., The Hierarchical System of the “Alphapro-teobacteria”: Description of Hyphomicrobiaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteri-aceae fam. nov, Int. J. System. Evol. Microbiol., 2005, vol. 55, pp. 1907–1919.
Wiege, J., Wilke, D., Baumgarten, J., Opitz, R., and Schlegel, H.G., Transfer of the Nitrogen-Fixing Hydrogen Bacterium Corynebacterium autotrophicum Baumgarten et. al. to Xanthobacter gen. nov., Int. System. Bacteriol., 1978, vol. 28, pp. 573–581.
Malik, K.A. and Claus, D., Xanthobacter flavus, a New Species of Nitrogen-Fixing Hydrogen Bacteria, Int. System. Bacteriol., 1979, vol. 29, pp. 283–287.
Jenni, B. and Arano, M., Xanthobacter agilis sp. nov., a Motile, Dinitrogen-Fixing, Hydrogen-Oxidizing Bacterium, Syst. Appl. Microbiol., 1987, vol. 9, pp. 254–257.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © M.V. Zaichikova, Yu.Yu. Berestovskaya, V.N. Akimov, A.K. Kizilova, L.V. Vasilieva, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 1, pp. 89–95.
Rights and permissions
About this article
Cite this article
Zaichikova, M.V., Berestovskaya, Y.Y., Akimov, V.N. et al. Xanthobacter xylophilus sp. nov., a member of the xylotrophic mycobacterial community of low-mineral oligotrophic waters. Microbiology 79, 83–88 (2010). https://doi.org/10.1134/S002626171001011X
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S002626171001011X