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Pseudomonas syringae lipopolysaccharides: Immunochemical characteristics and structure as a basis for strain classification

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Abstract

Lipopolysaccharide (LPS) preparations of 34 Pseudomonas syringae strains of 19 pathovars were prepared by saline extraction from wet cells and purified by repeated ultracentrifugation. The preparations reacted with homologous O-antisera, obtained by rabbit immunization with heat-killed bacterial cells. Through inhibition of homologous reactions between LPS preparations of heterologous strains (enzyme immunoassay, EIA), it was established for the first time that high serological affinity between strains is observed only if their LPS contains O-specific polysacc haride chains (OPS) comprised of completely identical rather than partially similar units. The central linear part of the OPS was found to be serologically inert when shielded with side groups. Data on immunochemical characteristics of the LPS and OPS structure are analyzed in relation to the design of P. syringae classification scheme.

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Authors and Affiliations

  1. Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kyiv, 03143, Ukraine

    G. M. Zdorovenko

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

    E. L. Zdorovenko

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  1. G. M. Zdorovenko
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  2. E. L. Zdorovenko
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Correspondence to E. L. Zdorovenko.

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Original Russian Text © G.M. Zdorovenko, E.L. Zdorovenko, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 1, pp. 52–62.

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Zdorovenko, G.M., Zdorovenko, E.L. Pseudomonas syringae lipopolysaccharides: Immunochemical characteristics and structure as a basis for strain classification. Microbiology 79, 47–57 (2010). https://doi.org/10.1134/S0026261710010078

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