Abstract
The enzyme immunoassay of soil suspensions was performed using antibodies against the lipopolysaccharides of Azospirillum brasilense model strains in order to assess the monthly population dynamics of the A. brasilense Sp245 introduced into the soil samples in laboratory simulation experiments and to determine the abundance of azospirilla in the various soils of the Saratov oblast. In laboratory simulation experiments (a sample of southern chernozem was used), the maximum amount of the antigen in question was detected in soil suspensions on day 7 of the one-month experiment on the introduction of bacterial cells into soil samples. Analysis of the major soil types of the Saratov oblast (saline, alluvial, and grey forest soils, as well as typical and southern chernozem) revealed the predominance of azospirilla antigens of the Sp245 serotype; significant amounts of the serotype Sp7 antigens were detected in southern chernozem.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Okon, Y. and Vanderleyden, J., Root-Associated Azospirillum Species Can Stimulate Plants, ASM News, 1997, vol. 63, pp. 366–370.
Bashan, Y., Holguin, G., and de-Bashan, L.E., Azospirillum-Plant Relationships: Physiological, Molecular, Agricultural, and Environmental Advances (1997–2003), Can. J. Microbiol., 2004, vol. 50, pp. 521–577.
Levanony, H., Bashan, Y., and Kahana, Z.E., Enzyme-Linked Immunosorbent Assay for Specific Identification and Enumeration of Azospirillum brasilense Cd in Cereal Roots, Appl. Environ. Microbiol., 1987, vol. 53, pp. 358–364.
Matora, L.Yu., Bogatyrev, V.A., Dykman, L.A., and Shchegolev, S.Yu., Immunochemical Identification of Azospirilla and Investigation of Their Antigenic Structures, in Molekulyarnye osnovy vzaimootnoshenii assotsiativnykh mikroorganizmov s rasteniyami (Molecular Basis of Relationship between Plants and Associated Microorganisms), Ignatov, V.V., Ed., Moscow: Nauka, 2005, pp. 209–237.
Guliy, O.I., Matora, L.Yu., Burygin, G.L., Dykman, L.A., Ostudin, N.A., Bunin, V.D., Ignatov, V.V., and Ignatov, O.V., Electrophysical Characteristics of Azospirillum brasilense Sp245 during Interaction with Antibodies to Various Cell-Surface Epitopes, Anal. Biochem., 2007, vol. 370, pp. 201–205.
Shelud’ko, A.V., Kulibyakina, O.V., Shirokov, A.A., Petrova, L.P., Matora, L.Yu., and Katsy, E.I., The Effect of Mutations Affecting Synthesis of Lipopolysaccharides and Calcofluor-Binding Polysaccharides on Biofilm Formation by Azospirillum brasilense, Mikrobiologiya, 2008, vol. 77, no. 3, pp. 358–363 [Microbiology (Engl. Transl.), vol. 77, no. 3, pp. 313–317].
Matora, L.Yu., Shvartsburd, B.I., and Shchegolev, S.Yu., Immunochemical Analysis of O-Specific Polysaccharides from the Soil Nitrogen-fixing Bacterium Azospirillum brasilense, Mikrobiologiya, 1998, vol. 67, no. 6, pp. 815–820 [Microbiology (Engl. Transl.), vol. 67, no. 6, pp. 677–681].
Matora, L.Yu., Burygin, G.L., and Shchegolev, S.Yu., Study of Immunochemical Heterogeneity of Azospirillum brasilense Lipopolysaccharides, Mikrobiologiya, 2008, vol. 77, no. 2, pp. 196–200 [Microbiology (Engl. Transl.), vol. 77, no. 2, pp. 166–170].
Konnova, O.N., Boiko, A.S., Burygin, G.L., Fedonenko, Yu.P., Matora, L.Yu., Konnova, S.A., and Ignatov, V.V., Chemical and Serological Studies of Lipopolysaccharides of Bacteria of the Genus Azospirillum, Mikrobiologiya, 2008, vol. 77, no. 3, pp. 350–357 [Microbiology (Engl. Transl.), vol. 77, no. 3, pp. 305–312].
Shirokov, A.A., Immunochemical Identification of Azospirillum brasilense Surface Structures Involved in Bacterial Social Behavior, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Saratov: IB PPM RAS, 2008.
Baldani, V.L.D., Baldani, J.I., and Döbereiner, J., Effects of Azospirillum Inoculation on Root Infection and Nitrogen Incorporation in Wheat, Can. J. Microbiol., 1983, vol. 29, pp. 924–929.
Döbereiner, J. and Day, J.M., Associative Symbiosis in Tropical Grass: Characterization of Microorganisms and Dinitrogen Fixing Sites, in Symposium on Nitrogen Fixation, Newton, W.E., and Nijmans, C.J., Eds., Pullman: Washington State Univ. Press, 1976, pp. 518–538.
Standifer, D.K., Immunoenzyme Analysis of Haptens and Antigens with Component Separation (Heterogenic Analysis), in Immunofermentnyi analiz (Immunoenzyme Analysis), Ngo, T.T. and Lenhoff, G., Eds., Moscow: Mir, 1988, pp. 172–192.
Pozdnyakova, L.I., Kanevskaya, S.V., Levanova, G.F., Barysheva, N.N., Pilipenko, T.Yu., Bogatyrev, V.A., and Fedorova, L.S., Taxonomic Study of the Azospirilla Isolated from the Cereals of the Saratov Oblast, Mikrobiologiya, 1988, vol. 57, no. 2, pp. 275–278.
Fedonenko, Yu.P., Borisov, I.V., Konnova, O.N., Zdorovenko, E.L., Katsy, E.I., Konnova, S.A., and Ignatov, V.V., Determination of the Structure of the Repeated Unit of the Azospirillum brasilense SR75 O-Specific Polysaccharide and Homology of the lps Loci in the Plasmids of Azospirillum brasilense Strains SR75 and Sp245, Mikrobiologiya, 2005, vol. 74, no. 5, pp. 626–632 [Microbiology (Engl. Transl.), vol. 74, no. 5, pp. 542–548].
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.I. Krasov, I.A. Popova, Yu.A. Filip’echeva, G.L. Burygin, L.Yu. Matora, 2009, published in Mikrobiologiya, 2009, Vol. 78, No. 5, pp. 662–666.
Rights and permissions
About this article
Cite this article
Krasov, A.I., Popova, I.A., Filip’echeva, Y.A. et al. Application of enzyme immunoassay for detection of the nitrogen-fixing bacteria of the genus Azospirillum in soil suspensions. Microbiology 78, 598–602 (2009). https://doi.org/10.1134/S0026261709050117
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026261709050117