Abstract
Antitumor activity of the preparation of the lipid fraction of Clostridium butyricum spore extract was demonstrated in vivo on a transplantable mouse model of breast cancer. At a specific scheme of application, inhibition of tumor growth and improved survival dynamics compared to the control group were observed. Thin-layer chromatography (TLC) of the lipid fraction of the spore extract revealed, apart from a saturated hydrocarbon, cholesterol ester, cetyl palmitate, triacyl glycerol, and palmitic acid, also a phenolic lipid bound in a complex with a peptide component. Acetone extraction of the lipid pool revealed a phenolic lipid. According to the TLC and 1H-NMR spectrum of the acetone extract of the lipid fraction of C. butyricum spores, the structure of the phenolic lipid was proposed, n -butyl benzoate substituted in the para position. The phenolic lipid is suggested to be responsible for the biological activity of the spore’s lipid fraction.
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Original Russian Text © G.P. Gaenko, E.V. Moiseeva, O.Yu. Savel’ev, Yu.G. Molotkovskii, E.L. Vodovozova, 2009, published in Mikrobiologiya, 2009, Vol. 78, No. 5, pp. 641–646.
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Gaenko, G.P., Moiseeva, E.V., Savel’ev, O.Y. et al. Antitumor activity of the lipid fraction of the spores of an anaerobic bacterium Clostridium butyricum . Microbiology 78, 580–584 (2009). https://doi.org/10.1134/S0026261709050087
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DOI: https://doi.org/10.1134/S0026261709050087