Abstract
The effect of the chemical analogues of microbial extracellular autoregulators belonging to alkylhydroxybenzenes (AHB), hexylresorcinol (HR), and methylresorcinol (MR), on the interactions between specific antibodies and the corresponding antigens was studied. Nonlinear dependency of the inhibition of binding of AHB-modified antibodies on the AHB chemical structures and concentrations was revealed by enzyme immunoassay. Hexylresorcinol was shown to decrease the antibody affinity and avidity indices and simultaneously increase the indices of nonspecific binding of AHB-modified antibodies to antigens, thereby promoting the formation of “false” antigen-antibody complexes. The nonspecificity of the influence of AHB on the antigenbinding capacity of antibodies is an important characteristic of these effects, which allows us to consider AHB as unique “superhaptenes”.
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Kozubek, A. and Tyman, J.H.P., Resorcinolic Lipids, the Natural Non-Isoprenoid Phenolic Amphiphiles and Their Biological Activity, Chem. Rev., 1999, vol. 99, no. 1, pp. 1–31.
Bespalov, M.M., Kolpakov, A.I., Loiko, N.G., Doroshenko, E.V., Mulyukin, A.L., Kozlova, A.N., Varlamova, E.A., Kurganov, B.I., and El’-Registan, G.I., The Role of Microbial Dormancy Autoinducers in Metabolism Blockade, Mikrobiologiya, 2000, vol. 69, no. 2, pp. 217–223 [Microbiology (Engl. Transl.), vol. 69, no. 2, pp. 174–179].
El’-Registan, G.I., Mulyukin, A.L., Nikolaev, Yu.A., Suzina, N.E., Gal’chenko, V.F., and Duda, V.I., Adaptogenic Functions of Extracellular Autoregulators of Microorganisms, Mikrobiologiya, 2006, vol. 75, no. 4, pp. 446–456 [Microbiology (Engl. Transl.), vol. 75, no. 4, pp. 380–389].
Soina, V.S., Mulyukin, A.L., Demkina, E.V., Vorobyova, E.A., and El-Registan, G.I., The Structure of Resting Bacterial Populations in Soil and Subsoil Permafrost, Astrobiology, 2004, vol. 4, pp. 345–358.
Suzina, N.E., Mulyukin, A.L., Dmitriev, V.V., Nikolaev, Yu.A., Shorokhova, A.P., Bobkova, Yu.S., Barinova, E.S., Plakunov, V.K., El-Registan, G.I., and Duda, V.I., The Structural Bases of Long-Term Anabiosis in Non-Spore-Forming Bacteria, J. Adv. Space Res, 2006, vol. 38, no. 6, pp. 1209–1219.
Vostroknutova, G.N., Kaprel’yants, A.S., Svetlichnyi, V.A., El’-Registan, G.I., Shevtsov, V.V., and Ostrokovskii, D.N., Membrane-Active Properties of the Preparation of Bacterial Culture Liquid with an Autoregulatory Activity, Prikl. Biokhim. Mikrobiol., 1983, vol. 19, no. 4, pp. 547–551.
Martirosova, E.I., Karpekina, T.A., and El’-Registan, G.I., Enzyme Modification by Natural Chemical Chaperons of Microorganisms, Mikrobiologiya, 2004, vol. 73, no. 5, pp. 708–715 [Microbiology (Engl. Transl.), vol. 73, no. 5, pp. 609–615].
Nikolaev, Yu.A., Loiko, N.G., Stepanenko, I.Yu., Shanenko, E.F., Martirosova, E.I., Plakunov, V.K., Kozlova, A.N., Borzenkov, I.A., Korotina, O.A., Rodin, D.S., Krupyanskii, Yu.F., and El’-Registan, G.I., Changes in Physicochemical Properties of Proteins, Caused by Modification with Alkylhydroxybenzenes, Prikl. Biokhim. Mikrobiol., 2008, vol. 44, no. 2, pp. 159–167 [Appl. Biochem. Microbiol. (Engl. Transl.), vol. 44, no. 2, pp. 143–150].
Davydova, O.K., Deryabin, D.G., Nikiyan, A.N., and El’-Registan, G.I., Mechanisms of Interaction between DNA and Chemical Analogues of Microbial Anabiosis Autoinducers, Mikrobiologiya, 2005, vol. 74, no. 5, pp. 616–625 [Microbiology (Engl. Transl.), vol. 74, no. 5, pp. 533–541].
Kolpakov, A.I., Il’inskaya, O.N., Bespalov, M.M., Kupriyanova-Ashina, F.G., Gal’chenko, V.F., Kurganov, B.I., and El’-Registan, G.I., Stabilization of Enzymes by Dormancy Autoinducers as a Possible Mechanism of Resistance of Resting Microbial Forms, Mikrobiologiya, 2000, vol. 69, no. 2, pp. 224–230 [Microbiology (Engl. Transl.), vol. 69, no. 2, pp. 180–185].
Kozubek, A. and Wroblewski, Z., Cereal Grain Long Chain Amphiphilic Resorcinolic Lipids Inhibit Significantly Binding of Fibrinogen by Platelets whereas Short Chain Resorcinolic Lipids and Fatty Acids Do not, Stud. Biophys., 1990, no. 139, pp. 177–181.
Immunologicheskie metody (Methods in Immunology) Frimel, G., Ed., Moscow: Meditsina, 1987.
Roit, A., Brostoff, Dzh., and Meil, D., Immunologiya. Per. s angl. (Immunology), Moscow: Mir, 2000.
Petrovskii, A.S., Deryabin, D.G., Loiko, N.G., Mikhailenko, N.A., Kobzeva, T.G., Kanaev, P.A., Nikolaev, Yu.A., Krupyanskii, Yu.F., Kozlova, A.N., and El’-Registan, G.I., Regulation of the Functional Activity of Lysozyme by Alkylhydroxybenzenes, Mikrobiologiya, 2009, vol. 78, no. 2, pp. 176–185 [Microbiology (Engl. Transl.), vol. 78, no. 2, pp. 144–153].
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Original Russian Text © D.G. Deryabin, N.A. Mikhailenko-(Romanenko), G.I. El’-Registan, 2009, published in Mikrobiologiya, 2009, Vol. 78, No. 5, pp. 629–635.
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Deryabin, D.G., Mikhailenko-(Romanenko), N.A. & El’-Registan, G.I. The effect of alkylhydroxybenzenes on the antigen-binding capacity of antibodies. Microbiology 78, 569–574 (2009). https://doi.org/10.1134/S0026261709050063
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DOI: https://doi.org/10.1134/S0026261709050063