Physiological and emission characteristics of the luminescent bacterium Photobacterium Phosphoreum from the White Sea


Growth and emission characteristics of the luminescent bacterium Photobacterium phosphoreum strain KM MGU 331 originating from the White Sea and isolated from the intestine of a bottom-dwelling fish, the European sculpin, Myoxocephalus scorpius, were analyzed. The strain is characterized by a high rate of colony formation and high intensity of light emission on agarized medium at 4° C as well as by highly efficient (5 × 105 quanta s−1 cell−1) and prolonged (over 100 h) light generation upon submerged cultivation at 20°C. The acidic shift of pH in the medium didn’t exceed 0.3 pH units. Effects of temperature, pH, and sodium chloride concentration on emission characteristics of intact photobacterium cells were studied. The optimal temperature for luminescence was found to be 15°C. The maximum luminescence activity was stable in a wide pH range from 7.0 to 9.0. Luminescence occurred within the range of 0.2–3.8% NaCl with the maximum at 2.5%. The results obtained confirm the literature data suggesting that luminescent bacteria adapted to low-temperature conditions possess a highly conjugated system of electron transfer to luciferase.

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  1. 1.

    Nealson, K.H., and Hastings, J.W., Bacterial Bioluminescence: Its Control and Ecological Significance, Microbiol. Rev., 1979, vol. 43, no. 4, pp. 406–518.

    Google Scholar 

  2. 2.

    Nealson, K.H., Isolation, Identification, and Manipulation of Luminous Bacteria, Meth. Enzymol., 1978, vol. 57, pp. 153–166.

    Article  CAS  Google Scholar 

  3. 3.

    Gitelson, J.I., Vydryakova, G.A., Kuznetsov, A.M., Rodicheva, E.K., Medvedeva, S.E., and Chugaeva, Yu.V., Luminous Bacteria Culture Collection Biodiversity and Applied aspects, Biolum. Chemilum., Case, J.F., et al., Eds., Singapore: World Sci. Publ. Co., 2000.

    Google Scholar 

  4. 4.

    Ruby, E.G., and Nealson, K.H., Seasonal Changes in the Species Composition of Luminous Bacteria in the Nearshore Seawater, Luminol. Oceanogr., 1978, vol. 23, pp. 164–169.

    Google Scholar 

  5. 5.

    Ruby, E.G., Greenberg, E.P., and Hastings, J.W., Planctonic Marine Luminous Bacteria: Species Distribution in the Water Colump, Appl. Environ. Microbiol., 1980, vol. 39, no. 2, pp. 302–306.

    PubMed  Google Scholar 

  6. 6.

    Shilo, M., and Yetinson, T., Physiological Characteristics Underlying the Distribution Patterns of Luminous Bacteria in the Mediterranean Sea and the Gulf of Elat, Appl. Environ. Microbiol., 1979, vol. 38, no. 4, pp. 577–584.

    PubMed  Google Scholar 

  7. 7.

    Hendrie, M.S., Hodgkiss, W., and Shewan, J.M., Identification, Taxonomy, and Classification of Luminous Bacteria, J. Gen. Microbiol., 1970, vol. 64, pp. 157–169.

    Google Scholar 

  8. 8.

    Wilson, Th., and Hastings, J.W., Bioluminescence, Annu. Rev. Cell Dev. Biol., 1998, vol. 14, pp. 197–230.

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    Hastings, J.W., and Nealson, K.H., Bacterial Bioluminescence, Annu. Rev. Microbiol., 1977, vol. 31, pp. 549–595.

    PubMed  Article  CAS  Google Scholar 

  10. 10.

    Watanabe, T., and Nakamura, T., Bioluminescence and Cell Growth of Photobacterium phosphoreum, J. Biochem., 1980, vol. 88, no. 3, pp. 815–817.

    PubMed  CAS  Google Scholar 

  11. 11.

    Johnson, F.H., Eyring, H., Steblye, R., Chaplin, H., Huber, C., and Gherardi, G., The Nature and Control of Reactions in Bioluminescence, J. Gen. Physiol., 1945, vol. 28, no. 5, pp. 463–537.

    Article  Google Scholar 

  12. 12.

    Cline, T.W., Isolation and Characterisation of Luminescence System Mutants in Bacteria, Meth. Enzymol., 1978, vol. 57, pp. 166–171.

    Article  CAS  Google Scholar 

  13. 13.

    Waters, P., and Lloyd, D., Salt, pH, and Temperature Dependencies of Growth and Bioluminescence of Three Species of Luminous Bacteria Analysed on Gradient Plates, J. Gen. Microbiol., 1985, vol. 11, no. 131, pp. 2865–2869.

    Google Scholar 

  14. 14.

    Hastings, J.W., and Weber, G., Total Quantum Flux of Isotropic Sources, Opt. Soc. Am., 1963, vol. 53, no. 12, pp. 1410–1415.

    Article  CAS  Google Scholar 

  15. 15.

    Watanabe, H., Mimura, M., Takimoto, A., and Nakamura, T., Luminescence and Respiratory Activities of Photobacterium phosphoreum, J. Biochem., 1975, vol. 77, no. 6, pp. 1147–1155.

    PubMed  CAS  Google Scholar 

  16. 16.

    Lee, B., Lee, J., Shin, D., and Kim, E., Statistical Optimization of Bioluminescence Photobacterium phosphoreum KCTC 2852, Environ. Int., 2006, vol. 32, no. 2, pp. 265–268.

    Article  Google Scholar 

  17. 17.

    Nealson, K.H., Platt, T., and Hastings, J.W., The Cellular Control of the Synthesis and Activity of the Bacterial Luminescent System, J. Bacteriol., 1970, vol. 104, no. 3, pp. 313–322.

    PubMed  CAS  Google Scholar 

  18. 18.

    Ulitzur, S., and Hastings, J.W., Growth, Luminescence Respiration, and the Adenosine Triphosphate Pool in Beneckea harveyi, J. Bact., 1978, vol. 133, pp. 1307–1313.

    PubMed  CAS  Google Scholar 

  19. 19.

    Makiguchi, N., Arita, M., and Asai, Y., Optimum Cultural Conditions for Strong Light Production by Photobacterium phosphoreum, J. Gen. Appl. Microbiol., 1980, vol. 26, no. 2, pp. 75–83.

    Article  CAS  Google Scholar 

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Correspondence to A. D. Ismailov.

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Original Russian Text © V.V. Kuts, A.D. Ismailov, 2009, published in Mikrobiologiya, 2009, Vol. 78, No. 5, pp. 612–617.

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Kuts, V.V., Ismailov, A.D. Physiological and emission characteristics of the luminescent bacterium Photobacterium Phosphoreum from the White Sea. Microbiology 78, 554 (2009).

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Key words

  • Photobacterium phosphoreum
  • luminescence
  • cultivation
  • temperature
  • pH
  • sodium chloride