Bacteria of the sulfur cycle in the sediments of gold mine tailings, Kuznetsk Basin, Russia
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The number and diversity of culturable microorganisms involved in sulfur oxidation and sulfate reduction were investigated in the oxidized sediments of gold mine tailings, Kuznetsk Basin, Russia. The sediments had a low pH (2.4–2.8), high SO 4 2− content (up to 22 g/l), and high concentrations of dissolved metals. The arsenic content was as high as 1.9 g/l. Bacterial phylogeny in microcosms was investigated by amplification of 16S rRNA gene fragments with subsequent denaturing gradient gel electrophoresis (DGGE). Spore-forming bacteria Desulfosporosinus were the only bacteria revealed for which the capacity for dissimilatory sulfate reduction is known. Strain Desulfosporosinus sp. DB was obtained in pure culture, and it was phylogenetically remote from other cultured and uncultured members of the genus. No sulfate-reducing members of the Deltaproteobacteria were detected. The Firmicutes members were the most numerous phylotypes in the microcosms, including a separate cluster with the similarity to Pelotomaculum not exceeding 94%. Acidithiobacillus ferrooxidans and A. caldus were found in anaerobic and microaerophilic microcosms. The number of sulfate reducers did not exceed 9.5 × 102 cells/ml.
Key wordsdenaturing gradient gel electrophoresis acid mine drainage sulfate-reducing bacteria gold mine tailings Acidithiobacillus Desulfosporosinus Pelotomaculum Thermincola
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- 2.Johnson, B., Biological Removal of Sulfurous Compounds from Inorganic Wastewaters, in Environmental technologies to Treat Sulfur Pollution: Principles and Engineering, Lens, P.N.L. and Hulshoff, L., Eds., London: IWA Publishing, 2000, pp. 175–205.Google Scholar
- 3.Gerashchenko, A.A., Analysis of the Mineral Source Base of Gold in the Kemerovo District, in Zoloto Kuzbassa (Gold of the Kuznetsk Basin), Kemerovo: Kemerovskii poligrafkombinat, 2000, pp. 69–213.Google Scholar
- 4.Widdel, F.F and Bak, R., Gram-Negative Mesophilic Sulfate-Reducing Bacteria, in The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd ed., Balows, A., et al., Eds., Berlin: Springer-Verlag, 1992, vol. 4, pp. 3352–3378.Google Scholar
- 5.Muyzer, G., Hottenträger, S., Teske, A., and Wawer, C., Denaturing Gradient Gel Electrophoresis of PCRAmplified 16S rDNA-a New Molecular Approach to Analyze the Genetic Diversity of Mixed Microbial Communities, in Molecular Microbial Ecology Manual, Akkermans, A.D.L., et al., Eds., Dordrecht: Kluwer Academic Publishers, 1996, pp. 1–23.Google Scholar
- 9.Hamamura, N., Olson, S.H., Ward, D.M., and Inskeep, W.P., Diversity and Functional Analysis of Bacterial Communities Associated with Natural Hydrocarbon Seeps in Acidic Soils at Rainbow Springs, Yellowstone National Park, Appl. Environ. Microbiol., 2005, vol. 71, pp. 5943–5950.PubMedCrossRefGoogle Scholar
- 11.Labrenz, M. and Banfield, J.F., Sulfate-Reducing Bacteria-Dominated Biofilms That Precipitate ZnS in a Subsurface Circumneutral-pH Mine Drainage System, Microbial Ecol., 2004, vol. 47, pp. 205–217.Google Scholar
- 17.Petrie, L., North, N.N., Dollhopf, S.L., Balkwill, D.L., and Kostka, J.E., Enumeration and Characterization of Iron(III)-Reducing Microbial Communities from Acidic Subsurface Sediments Contaminated with Uranium(VI), Appl. Environ. Microbiol., 2003, vol. 69, pp. 7467–7479.PubMedCrossRefGoogle Scholar
- 20.Sokolova, T.G., Kostrikina, N.A., Chernyh, N.A., Kolganova, T.V., Tourova, T.P., and Bonch-Osmolovskaya, E.A., Thermincola carboxydiphila gen. nov., sp. nov., a Novel Anaerobic, Carboxydotrophic, Hydrogenogenic Bacterium from a Hot Spring of the Lake Baikal Area, Int. J. Syst. Evol. Microbiol, 2005, vol. 55, pp. 2069–2073.PubMedCrossRefGoogle Scholar
- 21.Zavarzina, D.G., Sokolova, T.G., Tourova, T.P., Chernyh, N.A., Kostrikina, N.A., and Bonch-Osmolovskaya, E.A., Thermincola ferriacetica sp. nov., a New Anaerobic, Thermophilic, Facultatively Chemolithoautotrophic Bacterium Capable of Dissimilatory Fe(III) Reduction, Extremophiles, 2007, vol. 11, pp. 1–7.PubMedCrossRefGoogle Scholar