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Estimation of abundance dynamics of gram-negative bacteria in soil

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Bacterial succession in soil was studied for two variants of initiation (moistening and moistening with addition of glucose). To determine the numbers of viable gram-negative bacteria, the modified nalidixic acid method was applied. The numbers of gram-negative bacteria revealed by this method were 2 to 3.5 times higher than those determined by the traditional method. In a developing community, the highest total bacterial numbers were observed on day 7; afterwards their numbers decreased and stabilized at a level exceeding four-to fivefold the initial one. In both experimental variants, the highest numbers of viable gram-negative bacteria were revealed on day 15 (75–85% of the total bacterial numbers). Morphology of these bacteria suggests their classification as cytophagas (chitinophagas) utilizing chitin from the dead fungal mycelium.

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Original Russian Text © L.M. Polyanskaya, K.E. Ivanov, V.S. Guzev, D.G. Zvyagintsev, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 6, pp. 848–853.

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Polyanskaya, L.M., Ivanov, K.E., Guzev, V.S. et al. Estimation of abundance dynamics of gram-negative bacteria in soil. Microbiology 77, 760–764 (2008). https://doi.org/10.1134/S0026261708060143

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