Abstract
Bacterial succession in soil was studied for two variants of initiation (moistening and moistening with addition of glucose). To determine the numbers of viable gram-negative bacteria, the modified nalidixic acid method was applied. The numbers of gram-negative bacteria revealed by this method were 2 to 3.5 times higher than those determined by the traditional method. In a developing community, the highest total bacterial numbers were observed on day 7; afterwards their numbers decreased and stabilized at a level exceeding four-to fivefold the initial one. In both experimental variants, the highest numbers of viable gram-negative bacteria were revealed on day 15 (75–85% of the total bacterial numbers). Morphology of these bacteria suggests their classification as cytophagas (chitinophagas) utilizing chitin from the dead fungal mycelium.
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Polyanskaya, L.M. and Zvyagintsev, D.G., Microbial Succession in Soil, Soviet Scientist reviews. Harwood Academic Publ. Gmbh., 1994, no. 1, pp. 1–65.
Zvyagintsev, D.G., Bab’eva, I.P., Dobrovol’skaya, N.G., Zenova, G.M., and Mirchink, T.G., Vertical Layered Organization of Microbial Communities in Forest Biogeocenoses, Mikrobiologiya, 1993, vol. 62, no. 1, pp. 5–36.
Polyanskaya, L.M., Mirchink, T.G., Kozhevin, P.A., and Zvyagintsev, D.G., Structural Changes of the Soil Mycromycete Complex in the Course of Microbial Successions, Mikrobiologiya, 1990, vol. 59, no. 2, pp. 349–354.
Zvyagintsev, D.G., Bab’eva, I.P., Zenova, G.M., and Polyanskaya, L.M., Diversity of Fungi and Actinomycetes and Their Ecological Functions, Pochvovedenie, 1996, no. 6, pp. 705–713 [Eur. Soil Sci. (Engl. Transl.), no. 6, pp. 635–642].
Polyanskaya, L.M., Orazova, M.Kh., Mirchink, T.G., and Zvyagintsev, D.G., Abundance Dynamics and Structure of the Pea Root Zone Microbial Complex, Mikrobiologiya, 1994, vol. 63, no. 2, pp. 314–325.
Zvyagintsev, D.G. and Zenova, G.M., Ekologiya aktinomitsetov (Ecology of Actinomycetes), Moscow: GEOS, 2001.
Kozhevin, P.A., Polyanskaya, L.M., and Zvyagintsev, D.G., Dynamics of Development of Different Microorganisms in Soil, Mikrobiologiya, 1979, vol. 48, no. 4, pp. 490–499.
Kochkina, G.A., Succession of Soil Microorganisms and Related Placement of Specific Microbial Populations, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Moscow: Mosk. Gos. Univ., 1981.
Kozhevin, P.A., Mikrobnye populyatsii v prirode, (Microbial Populations in Nature), Moscow: Mosk. Gos. Univ., 1989.
Zvyagintsev, D.G., Ultrasound Soil Pretreatment for Quantification of Microorganisms, Vestn. Mosk. Univ., Ser. Biol. Soil Sci., 1968, no. 3, pp. 127–129.
Metody pochvennoi mikrobiologii i biokhimii (Methods of Soil Microbiology and Biochemistry) Zvyagintsev, D.G., Ed., Moscow: Mosk. Gos. Univ., 1991.
Kogure, K., Simidu, V., and Taga, N., A Tentative Direct Microscopic Methods for Counting Living Marine Bacteria, Can. J. Microbiol., 1979, vol. 25, pp. 415–420.
Fry, J.C., Direct Methods and Biomass Estimation, in Methods in Microbiology, Grigorova, R. and Norris, Y.K., Eds., London: Acad. Press, 1990, vol. 22, pp. 41–81.
Dobrovol’skaya, T.G., Struktura bakterial’nykh soobshchestv pochv (Structure of Soil Bacterial Communities), Moscow: IKTs “Akademkniga”, 2002.
Polyanskaya, L.M., Microbial Succession in Soil, Extended Abstract of Doctoral (Biol.) Dissertation, Moscow: Mosk. Gos. Univ., 1996.
Polyanskaya, L.M. and Zvyagintsev, D.G., Population Ecology of Soil Actinomycetes and its Role in the Regulation of the Quantity of the Soil Microbial Complex, Izv. Akad. Nauk SSSR, Ser. Biol., 1984, no. 5, pp. 746–753.
Magda Mirgani, Polyanskaya, L.M., and Zvyagintsev, D.G. Characterization of the Soil Actinomycete Complex by Its Radial Growth Rate, Vestnik MGU. Ser. Pochvovedenie, 1986, vol. 17, no. 3, pp. 33–38.
Polyanskaya, L.M., Triger, E.G., Kozhevin, P.A., and Zvyagintsev, D.G., Kinetic description of the Structure of the Soil Actinomycete Complex, Mikrobiologiya, 1988, vol. 57, no. 5, pp. 854–858.
Zvyagintsev, D.G., Bab’eva, I.P., and Zenova, G.M., Biologiya pochv (Soil Biology), Moscow: Mosk. Gos. Univ., 2005.
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Original Russian Text © L.M. Polyanskaya, K.E. Ivanov, V.S. Guzev, D.G. Zvyagintsev, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 6, pp. 848–853.
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Polyanskaya, L.M., Ivanov, K.E., Guzev, V.S. et al. Estimation of abundance dynamics of gram-negative bacteria in soil. Microbiology 77, 760–764 (2008). https://doi.org/10.1134/S0026261708060143
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DOI: https://doi.org/10.1134/S0026261708060143