Abstract
Bacilli of the species Bacillus subtilis, B. pumilus, B. mycoides, B. marinus and B. licheniformis (a total of 53 strains) were isolated from 15 invertebrate species and the water of the Vostok Bay, Peter the Great Bay, Sea of Japan. Bacilli were most often isolated from bivalves (22.7%) and sea cucumbers (18.9%); they occurred less frequently in sea urchins and starfish (13.2 and 7.5%, respectively). Most of bacilli strains were isolated from invertebrates inhabiting silted sediments. No Bacillus spp. strains were isolated from invertebrates inhabiting stony and sandy environments. The species diversity of bacilli isolated from marine objects under study was low. Almost all bacterial isolates were resistant to lincomycin. Unlike B. pumilus, B. subtilis isolates were mostly resistant to benzylpenicillin and ampicillin. Antibiotic sensitivity of B. licheniformis strains was variable (two strains were resistant to benzylpenicillin and oxacillin, while one was sensitive). A significant fraction of isolated bacilli contained pigments. Pigmented strains were more often isolated from seawater samples, while colorless ones predominated within hydrobionts. B. subtilis colonies had the broadest range of colors. In the Bacillus strains obtained, DNase, RNase, phosphatase, elastolytic, chitinase, and agarolytic activity was detected. Bacilli strains with hydrolytic activity occurred in invertebrates more often than in seawater.
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Boudabous, A. and Bianchi, A., Taxonomic et ecologie des du genre Bacillus isolees du milier marin, Resumes Colloque. “Microbiologue Marine” Sosiete Francaise de Microbiologie. Marseille, 1976, pp. 41–42.
Boeye, H. and Aerts, M., Numerical Taxonomy of Bacillus Isolates from North Sea Sediments, Int. J. Syst. Bacteriol., 1976, vol. 26, pp. 427–441.
Shotts, E.B., Albert, T.F., Wooly, R.E., and Brown, J., Microflora Associated with the Skin of the Bowhead Whale (Balaena mysticetus), J. Wildlife Dis, 1990, vol. 26, pp. 351–359.
Moeler, R., Horneck, G., Faclus, R., and Stackebrandt, E., Role of Pigmentation in Protecting Bacillus sp. Endospores Against Environmental UV Radiation, FEMS Microbiol. Ecol., 2005, vol. 51, no. 2, pp. 231–236.
Nicholson, W.L., Munakata, N., Horneck, G., Melosh, H.J., and Setlow, P., Resistance of Bacillus Endospores to Extreme Terrestrial and Extraterrestrial Environmental, Microbiol. Mol. Biol. Rev, 2000, vol. 64, no. 3, pp. 548–572.
Mikhailov, V.V., Biota Rossiiskikh vod Yaponskogo morya. T. 2. Prokarioty (Biota of the Russian Waters of the Sea of Japan, vol. 2. Prokaryotes), Vladivostok: Dal’nauka, 2004.
Spelecky, R.A. and Hemphill, H.E., The Genus Bacillus-nonmedical, in The Prokaryotes. 2nd ed, Ballows, A., Truper, H.G., Dworkin, M., Harber, H., and Schleifer, K.-H., Eds., Berlin: Springer, 1992 (http://141.150.157.117.8080/proPUB/index.html).
Zhuang, W.Q., Maszenan, A.M., Krumholz, L.R., and Tay, S.T., Importance of Gram-Positive Naphthalene-Degrading Bacteria in Oil-Contaminated Tropical Marine Sediments, Lett. Appl. Microbiol, 2003, vol. 36, pp. 251–257.
Ivanova, E.P., Kuznetsova, T.A., and Mikhailov, V.V., Fungicidal Compounds Synthesized by Bacteria Associated with Verongia sp. Marine Sponges, Biol. Morya, 2000, vol. 26, no. 3, pp. 116–118.
Thakur, N.L., Anil, A.C., and Müller, W.E.G., Culturable Epibacteria of the Marine Sponge Ircinia fusca: Temporal Variations and Their Possible Role in the Epibacterial Defense of the Host, Aquat. Microbial. Ecol, 2004, vol. 37, pp. 295–304.
Jensen, P.R. and Fenical, W., Strategies for the Discovery of Secondary Metabolities from Marine Bacteria: Ecological Perspectives, Annu. Rev. Microbiol., 1994, vol. 48, pp. 559–584.
Irianto, A., Robrertson, P.A.W., and Austin, B., The Use of Probiotics in Aquaculture, Recent Res. Devel. Microbiol, 2000, vol. 4, pp. 557–567.
Trebukhova, Yu.A. and Pavlyuk, O.N., Species Composition and Distribution of Free-Living Marine Nematodes in the Vostok Bay (Sea of Japan), Biol. Morya, 2006, vol. 32, no. 1, pp. 8–16.
Beleneva, I.A., Zhukova, N.V., Le Lan H., Nguyen D.H., Taxonomic Composition of Bacteria Associated with Cultivated Mollusks Crassostrea lugubris and Perna viridis and with the Water of the Gulf of Nha Trang Lagoon, Vietnam, Microbiology, 2007, vol. 76, pp. 220–228.
Claus, D. and Berkeley, R.C.W., Genus Bacillus, Cohn 1872, in Bergey’s Manual of Systematic Bacteriology, Baltimore: Williams and Wilkins, 1986, vol. 2, pp. 1105–1139.
Lutsik, N.V., Seasonal Changes in ATP, Chlorophyll A, and Organic Carbon Content in Soft Sediments of the Vostok Bay (Sea of Japan), Biol. Morya, 1981, no. 1, pp. 38–42.
Ivanova, E.P., Mikhailov, V.V., and Andreev, L.V., Marine Bacilli and Some Approaches to Their Identification, Mikrobiol. Zh., 1992, vol. 54, no. 1, pp. 27–33.
Yoon, Y.-H., Weiss, N., Lee, K.-C., Lee, I.-S., Kang, K.H., and Park, Y.-H., Jeotgalibacillus alimentarius gen. nov., sp. nov., a Novel Bacterium Isolated from Jeotgal With, L-Lysine in the Cell Wall, and Reclassification of Bacillus marinus Röger 1983 as Marinibacillus marinus gen. nov., comb. nov., Int. J. Syst. Evol. Microbiol., 2001, vol. 51, pp. 2087–2093.
Rüger, H-J. and Richter, G., Bacillus globisporus subsp. marinus subsp. nov, Int. J. Syst. Bacteriol., 1979, vol. 29, pp. 196–203.
Rüger, H-J., Fritze, D., and Sproer, C., New Psychrophilic and Psychrotolerant Bacillus marinus Strains from Tropical and Polar Deep-Sea Sediments and Emended Description of the Species, Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 1305–1313.
Egan, S., James, S., Holmstrom, C., and Kjelleberg, S., Correlation Between Pigmentation and Antifouling Compounds Produced by Pseudoalteromonas tunicate, Environ. Microbiol, 2002, vol. 4, pp. 433–442.
Reva, O.N., Vyunitskaya, V.A., Reznik, S.R., Kozachko, I.A., and Smirnov, V.V., Antibiotic Susceptibility as a Taxonomic Characteristic of the Genus Bacillus, Int. J. Syst.Bacteriol, 1995, vol. 45, no. 2, pp. 409–411.
Ivanova, E.P., Vysotskii, M.V., Svetashev, V.I., Nedashkovskaya, O.I., Gorshkova, N.M., Mikhailov, V.V., Yumoto, N., Shigeri, Y., Taguchi, T., and Yoshikawa, S., Characterization of Bacillus Strains of Marine Origin, Int. Microbiol., 1999, vol. 2, pp. 267–271.
Mikhailov, V.V., Kuznetsova, T.A., and Elyakov, G.B., Morskie mikroorganizmy i ikh vtorichnye biologicheski aktivnye metabolity (Marine Microorganisms and Their Secondary Biologically Active Metabolites), Vladivostok: Dal’nauka, 1999.
Bakunina, I.Yu., Shevchenko, L.S., Nedashkovskaya, O.I., Shevchenko, N.M., Alekseeva, S.A., Mikhailov, V.V., and Zvyagintseva, T.N., Screening of Marine Bacteria for Fucoidanases, Mikrobiologiya, 2000, vol. 69, no. 3, pp. 370–376 [Microbiology (Engl. Transl.), vol. 69, no. 3, pp. 303–308].
Ivanova, E.P., Frolova, G.M., Mikhailov, V.V., and Gorshkova, N.M., Directed Screening for Alkaline Phosphatases of Microbial Origin, Prikl. Biokhim. Mikrobiol., 1992, vol. 28, no. 5, pp. 726–730.
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Original Russian Text © I.A. Beleneva, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 4, pp. 558–565.
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Beleneva, I.A. Distribution and characteristics of Bacillus bacteria associated with hydrobionts and the waters of the Peter the Great Bay, Sea of Japan. Microbiology 77, 497–503 (2008). https://doi.org/10.1134/S0026261708040176
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DOI: https://doi.org/10.1134/S0026261708040176