Skip to main content
SpringerLink
Log in

Characterization of an extracellular glycolipid from Lentinus edodes (Berk.) Sing [Lentinula edodes (Berk.) Pegler]

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

Submerged mycelium of a xylotrophic basidiomycete Lentinus edodes produces an extracellular glycolipid, S3, associated with a lectin. Galactose glycan residue, as well as the lipid pool composition, which includes nonhydroxylated short-chain fatty acids, is uncommon for basidiomycetes. The glycolipid consists of D-galactopyranose (15% of S3 contains galactose sulfate) acylated by octadecanoic and nonadecanoic fatty acid residues (28 and 72%, respectively). The glycolipid structure and composition are confirmed by physicochemical analysis. The glycolipid is assumed to be a regulator of lectin activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kulakovskaya, E.V., Kulakovskaya, T.V., Golubev, V.I., Shashkov, A.S., Grachev, A.A., and Nifant’ev, N.E., Fungicidal Activity of Cellobiose Lipids from Culture Broth of Yeast Cryptococcus humicola and Pseudozyma fusiformata, Bioorg. Khim., 2007, vol. 33, no. 1, pp. 167–171 [Russ. J. Bioorg. Chem. (Engl. Transl.), vol. 33, no. 1, pp. 156–160].

    Google Scholar 

  2. Golubev, W.I., Kulakovskaya, T.V., and Golubeva, E.V., The Yeast Pseudozyma fusiformata VKM Y-2821 Producing an Antifungal Glycolipid, Mikrobiologiya, 2001, vol. 70, no. 5, pp. 642–646 [Microbiology (Engl. Transl.), vol. 70, no. 5, pp. 553–556].

    CAS  Google Scholar 

  3. Chen, Y., McNally, D.J., Labbe, C., Voyer, N., Belzile, F., and Belanger, R.R., Insertional Mutagenesis of a Fungal Biocontrol Agent Led To Discovery of a Rare Cellobiose Lipid with Antifungal Activity, Appl. Environ. Microbiol., 2003, vol. 69, no. 5, pp. 2595–2602.

    Article  Google Scholar 

  4. Puchkov, E.O. and Zahringer, U., Lindner, B., Kulakovskaya, T.V., Seydel, U., and Wiese, A., Mycocidal, Membrane-Active Complex of Cryptococcus humicola, a New Type of Cellobiose Lipid with Detergent Features, Biochem. Biophys. Acta (Biomembranes), 2002, vol. 1558, no. 2, pp. 161–170.

    Article  CAS  Google Scholar 

  5. Golubev, W.I., Kulakovskaya, T.V., Kulakovskaya, E.V., and Golubev, H.R., The Fungicidal Activity of an Extracellular Glycolipid from Sympodiomycopsis paphiopedili Sugiyama et al., Mikrobiologiya, 2004, vol. 73, no. 6, pp. 841–845 [Microbiology (Engl. Transl.), vol. 73, no. 6, pp. 724–728].

    CAS  Google Scholar 

  6. Konova, I.V., Karpova, N.V., Tkachevskaya, E.P., and Alekseev, S., Phenol-Containing Glycolipids in the Lipid Fractions of Heterothallic Strains of the Zygomycete Blakeslea trispora, Mikrobiologiya, 1991, vol. 60, no. 1, pp. 85–91.

    CAS  Google Scholar 

  7. Jeune, K.H., Moon, I.J., Kim, M.K., and Chung, S.R., Studies on Lectins from Korean Higher Fungi; IV. A Mitogenic Lectin from the Mushroom Lentinus edodes, Planta Med., 1990, vol. 56, p. 592.

    Article  Google Scholar 

  8. Wang, H.X., Ng, T.B., and Ooi, V.E.C., Studies on Purification of a Lectin from Fruiting Bodies of the Edible Shiitake Mushroom Lentinus edodes, Int. J. Biochem. Cell Biol, 1999, vol. 31, no. 5, pp. 595–599.

    Article  CAS  Google Scholar 

  9. Tsivileva, O.M., Nikitina, V.E., Garibova, L.V., Zav’yalova, L.A., and Ignatov, V.V., Hemagglutinating Activity of the Fungus Lentinus edodes (Berk.) Sing [Lentinus edodes (Berk.) Pegler], Mikrobiologiya, 2000, vol. 69, no. 1, pp. 38–44 [Microbiology (Engl. Transl.), vol. 69, no. 1, pp. 30–35].

    CAS  Google Scholar 

  10. Tsivileva, O.M., Nikitina, V.E., Garibova, L.V., and Ignatov, V.V., Lectin Activity of Lentinus edodes, Int. Microbiol, 2001, vol. 4, no. 1, pp. 41–45.

    PubMed  CAS  Google Scholar 

  11. Nikitina, V.E., Tsivileva, O.M., Babitskaya, V.G., Shchebra, V.V., and Puchkova, T.A., Modern Biotechnological Aspects of Submerged Cultivation of Lentinus edodes, in Uspekhi Meditsinskoi Mikologii (Advances in Medical Mycology), vol. 9, Sergeev, Yu.V., Ed., Moscow: Natsional’naya akademiya mikologii, 2007, pp. 247–251.

    Google Scholar 

  12. Tsivileva, O.M., Nikitina, V.E., and Garibova, L.V., Effect of Culture Medium Composition on the Activity of Extracellular Lectins of Lentinus edodes, Prikl. Biokhim. Mikrobiol., 2005, vol. 41, no. 2, pp. 200–203 [Appl. Biochem. Microbiol. (Engl. Transl.), vol. 41, no. 2, pp. 174–176].

    PubMed  CAS  Google Scholar 

  13. Lutsik, M.D., Panasyuk, E.N., and Lutsik, A.D., Lektiny (Lectines), Lvov: Vishcha shkola, 1981.

    Google Scholar 

  14. Song, C.H., Cho, K.Y., Nair, N.G., and Vine, J., Growth Stimulation and Lipid Synthesis in Lentinus edodes, Mycologia, 1989, vol. 81, no. 4, pp. 514–522.

    Article  CAS  Google Scholar 

  15. Petsev, N. and Kotsev, N., Spravochnik po gazovoi khromatografii (Handbook on Gas Chromatography), Moscow: Mir, 1987.

    Google Scholar 

  16. Khromatografiya: Prakt. prilozhenie metoda (Chromatography: Practical Applications), Moscow: Mir, 1986.

  17. Tsivileva, O.M., Makarov, O.E., Nikitina, V.E., and Garibova, L.V., Changes in the Fatty Acid Composition of Total Lipids and Lectin Activity in the Course of Lentinus edodes Morphogenesis, Mikol. Fitopatol., 2007, vol. 41, no. 5, pp. 450–455.

    Google Scholar 

  18. Nikitina, V.E., Tsivileva, O.M., and Garibova, L.V., Stimulators of Lentinus edodes Lectin Activity on Synthetic Agarized Media, Biotekhnol., 2004, no. 3, pp. 49–54.

  19. Andreev, V.S., Popov, V.G., and Dronova, N.V., Electrobiochemical Regulation Mechanism of Microbial Physiological Activity on the Population Level, Biotekhnol., 1988, vol. 4, no. 1, pp. 32–36.

    CAS  Google Scholar 

  20. Feofilova, E.P., Biochemical Adaptation of Fungi to Thermal Stress, Mikrobiologiya, 1994, vol. 63 no. 5, pp. 757–776.

    CAS  Google Scholar 

  21. Pankratov, A.N. and Ostroumov, I.G., Ustanovlenie stroeniya molekul fizicheskimi metodami (Determination of Molecul;ar Structure by Physical Methods), Saratov: Saratovskogo Univ., 1995.

    Google Scholar 

  22. Vorob’eva, E.V., Krasikova, I.N., Dmitrenok, P.S., Dmitrenok, P.S., Isakov, V.V., Nedashkovskaya, O.I., and Solov’eva, T.F., An Unusual Lipid A from a Marine Bacterium Chryseobacterium scophtalmum CIP 104199T, Bioorg. Khim., 2006, vol. 32, no. 5, pp. 538–545 [Russ. J. Bioorg. Chem. (Engl. Transl.), vol. 32, no. 5, pp. 485–491].

    PubMed  CAS  Google Scholar 

  23. Pluzhnikov, K.A., Bocharov, D.N., Kononova, N.V., Balashova, T.A., Arsen’ev, A.S., and Grishin, E.V., Identification and Structural Analysis of a Glycophospholipid Component from the Venom of Ant Paraponera clavata, Bioorg. Khim., vol. 32, no. 5, pp. 530–537 [Russ. J. Bioorg. Chem. (Engl. Transl.), vol. 32, no. 5, pp. 478–484].

Download references

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410049, Russia

    O. M. Tsivileva, V. E. Nikitina & O. E. Makarov

Authors
  1. O. M. Tsivileva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. E. Nikitina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. E. Makarov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. M. Tsivileva.

Additional information

Original Russian Text © O.M. Tsivileva, V.E. Nikitina, O.E. Makarov, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 4, pp. 490–495.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsivileva, O.M., Nikitina, V.E. & Makarov, O.E. Characterization of an extracellular glycolipid from Lentinus edodes (Berk.) Sing [Lentinula edodes (Berk.) Pegler]. Microbiology 77, 436–440 (2008). https://doi.org/10.1134/S0026261708040085

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261708040085

Key words

Search

Navigation