Abstract
Heat shock (45°C) and the effect of oxidants (H2O2) resulted in a decrease of the respiratory activity of yeast cells and their survival rate. Increased resistance to stress effects after mild heat treatment (37°C) or treatment with a nonlethal dose of oxidants (0.5 mM H2O2) for 60 min) was accompanied by appearance of an alternative (cyanide-resistant) oxidative pathway in the mitochondria, which promotes survival due to retention of the capacity for ATP synthesis in the first coupling point at the level of endogenous NADH dehydrogenase. The alternative oxidative pathway is more resistant to the effect of stressors that disrupt electron transfer in the cytochrome site of the respiratory chain.
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Kapoor, M., Sreenivasan, G.M., Goel, N., and Lewis, J., Development of Thermotolerance in Neurospora crassa by Heat Shock and Other Stresses Eliciting Peroxidase Induction, J. Bacteriol., 1990, vol. 172, no. 5, pp. 2798–2801.
Davidson, J.F., Whyte, B., Bissinger, P.H., and Schiestl, R.H., Oxidative Stress Is Involved in Heat-Induced Cell Death in Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. USA, 1996, vol. 93, no. 10, pp. 5116–5121.
Plesofsky-Vig, N. and Brambl, R., Gene Sequence and Analysis of Hsp30, a Small Heat Shock Protein of Neurospora crassa Which Associates with Mitochondria, J. Biol. Chem., 1990, vol. 265, pp. 15432–15440.
Ton-That, T.C., Michea-Hamzehpour, M., and Turian, G., Respiratory Response to Heat Shock in Neurospora crassa, Protoplasma, 1983, vol. 116, pp. 149–154.
Michea-Hamzehpour, M. and Turian, G., GMP-Stimulation of the Cyanide-Insensitive Mitochondrial Respiration in Heat-Shocked Conidia of Neurospora crassa, Experientia, 1987, vol. 43, pp. 439–440.
Habel, D., Plesofsky-Vig, N., and Brambl, R., The Respiratory Response to Heat Shock in Neurospora crassa, FEMS Microbiol. Letts., 1991, vol. 81, pp. 317–322.
Lenaz, G., Role of Mitochondria in Oxidative Stress and Ageing, Biochim. Biophys. Acta, 1998, vol. 1366, pp. 53–67.
Lindquist, S. and Creig, E.A., The Heat-Shock Proteins, Annu. Rev. Genet., 1988, vol. 22, pp. 631–677.
Plesofsky-Vig, N. and Brambl, R., Heat Shock Response of Neurospora crassa Protein Synthesis and Induction Thermotolerance, J. Bacteriol., 1985, vol. 162, pp. 1083–1091.
Medentsev, A.G. and Akimenko, V.K., Development and Activation of Cyanide-Resistant Respiration in the Yeast Yarrowia lipolytica, Biokhimiya, 1999, vol. 64, no. 8, pp. 1123–1131 [Biochemistry (Moscow) (Engl. Transl.), vol. 64, no. 8, pp. 945–951].
Biryukova, E.N., Medentsev, A.G., Arinbasarova, A.Yu., and Akimenko, V.K., Tolerance of the Yeast Yarrowia lipolytica to Oxidative Stress, Mikrobiologiya, 2006, vol. 75, no. 3, pp. 293–298 [Microbiology (Engl. Transl.), vol. 75, no. 3, pp. 243–247].
Collinson, L.P. and Dawes, I.W., Inducibility of the Response of Yeast Cell to Peroxide Stress, J. Gen. Microbiol., 1992, vol. 31, no. 1, pp. 77–79.
Rikhvanov, E.G., Varakina, N.N., Rusaleva, T.M., Rachenko, E.I., and Voinikova, V.K., The Absence of a Direct Relationship between the Ability of Yeasts to Grow at Elevated Temperatures and Their Survival after Lethal Heat Shock, Mikrobiologiya, 2003, vol. 72, no. 4, pp. 476–481 [Microbiology (Engl. Transl.), vol. 72, no. 4, pp. 423–428].
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Original Russian Text © E.N. Biryukova, A.G. Medentsev, A.Yu. Arinbasarova, V.K. Akimenko, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 4, pp. 448–452.
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Biryukova, E.N., Medentsev, A.G., Arinbasarova, A.Y. et al. Respiratory activity of yeast Yarrowia lipolytica under oxidative stress and heat shock. Microbiology 77, 395–399 (2008). https://doi.org/10.1134/S0026261708040024
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DOI: https://doi.org/10.1134/S0026261708040024