Abstract
Two cyanobacterial strains, Pseudanabaena sp. 0411 and Synechococcus sp. 0431, were isolated from a sample collected in the Kotel’nikovskii hot spring of the Baikal rift. According to the results of light and transmission electron microscopy, as well as of the phylogenetic analysis of the 16S rRNA gene, these cyanobacteria were classified as Pseudanabaena sp. nov. and Synechococcus bigranulatus Skuja. The constructed phylogenetic tree shows that the studied strains are positioned in the clades of cyanobacteria isolated from hydrothermal vents of Asia and New Zealand, separately from marine and freshwater members of these genera, including those isolated from Lake Baikal.
Similar content being viewed by others
References
Bakterial’naya paleontologiya (Bacterial Paleontology) Rozanov, Yu., Ed., Moscow: Paleontol. Inst. RAN, 2002.
Kondrat’eva, E.N., Maksimova, I.V., and Samuilov, V.D., Fototrofnye mikroorganizmy. Ucheb. posobie (Phototrophic Microorganisms. Tutorial), Moscow: Mosk. Gos. Univ., 1989.
Sergeev, V.N., Gerasimenko, L.M., and Zavarzin, G.A., The Proterozoic History and Present State of Cyanobacteria, Mikrobiologiya, 2002, vol. 71, no. 6, pp. 725–740 [Microbiology (Engl. Transl.), vol. 71, no. 6, pp. 623–637].
Komárek, J. and Anagnostidis, K., Cyanoprokaryota—1. Teil: Chroococcales, in Süßwasserflora von Mitteleuropa, Ettl, H., Gerloff, J., Heynig, H., and Mollenhauer, D., Eds., Band 19/1, Gustav Fischer, 1999.
Komárek, J. and Anagnostidis, K., Cyanoprokaryota—1. Teil 2: Oscillatoriales, in Süßwasserflora von Mitteleuropa, Buedel, B., Krienitz, L., Gaertner, G., and Schagerl, M., Eds., Band 19/2, Heidelberg: Elsevier/Spectrum, 2005.
Popovskaya, G.I. and Belykh, O.I., Stages of Investigation of the Lake Baikal Autotrophic Picoplankton, Gidrobiol. Zhurnal, 2003, vol. 39, pp. 12–24.
Tikhonova, I.V., Morphological and Genetic Properties of the Lake Baikal Picoplanktonic Cyanobacteria, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Irkutsk: IGU, 2006.
Baikal. Atlas (Lake Baikal. An Atlas), Moscow: Roskartografiya, 1993, p. 160.
Pinneker, E.V., Kustov, Yu.I., Kalashnikova, E.B., and Pavlova, S.E., Geochemistry of Medicinal Mineral Waters of the Baikal Region, Otechestvennaya Geologiya, 1995, no. 12, pp. 42–48.
Anagnostidis, K. and Komárek, J., Modern Approach To the Classification System of Cyanophytes. 3—Oscillatoriales, Arch. Hydrobiol., vol. 80, no. 1–4, pp. 327–472.
Hoffmann, L., Komárek, J., and Kaštovský, J., System of Cyanoprokaryotes (Cyanobacteria)—State in 2004, Abstr. 16th Symp. Int. Ass. Cyanophyte Res., Luxemburg, 2004, p. 42.
Kompantseva, E.I. and Gorlenko, V.M., Phototrophic Communities in Some Thermal Springs of Lake Baikal, Mikrobiologiya, 1988, vol. 57, no. 5, pp. 841–846.
Rippka, R., Isolation and Purification of Cyanobacteria, in Methods in Enzymology, Packer, L. and Glazer, A.N., Eds., vol. 167, pp. 3–27.
Shmidt, T.M., DeLong, E.F., and Pace, N.R., Analysis of a Marine Picoplankton Community by 16S rRNA Gene Cloning and Sequencing, J. Bacteriol., 1991, vol. 173, pp. 4371–4378.
Nübel, U., Garcia-Pichel, F., and Muyzer, G., PCR Primers To Amplify 16S rRNA Genes from Cyanobacteria, Appl. Environ. Microbiol., 1997, vol. 63, pp. 3327–3332.
Kimura, M., A Simple Method for Estimating Evolutionary Rates of Base Substitution Through Comparative Studies of Nucleotides Sequences, J. Molecular Evol., 1980, vol. 16, pp. 111–120.
Kumar, S., Tamura, K., and Nei, M., MEGA3: Integrated Software for Molecular Evolutionary Genetics Analysis and Sequence Alignment, Briefings in Bioinformatics, 2004, vol. 5, pp. 150–163.
Miller, S.R. and Castenholz, R.W., Ecological Physiology of Synechococcus sp. Strain SH-94-5, a Naturally Occurring Cyanobacterium Deficient in Nitrate Assimilation, Appl. Environ. Microbiol., 2001, vol. 67, pp. 3002–3009.
Lau, C.Y., Hongmei, J., Aitchison, J.C., and Pointing, S.B., Highly Diverse Community Structure in a Remote Central Tibetan Geothermal Spring Does Not Display Monotonic Variation to Thermal Stress, FEMS Microbiol. Ecol., 2006, vol. 57, pp. 80–91.
Hongmei, J., Aitchison, J.C., Lacap, D.C., Peerapornpisal, Y., Sompong, U., and Pointing, S.B., Community Phylogenetic Analysis of Moderately Thermophilic Cyanobacterial Mats from China, the Philippines and Thailand, Extremophiles, 2005, vol. 9, pp. 325–332.
Ng, C.C., Huang, W.C., Chang, C.C., Tzeng, W.S., Chen, T.W., Liu, Y.S., and Shyu, Y.T., Tufa Microbial Diversity Revealed by 16S rRNA Cloning in Taroko National Park, Taiwan, Soil Biol. Biochem., 2006, vol. 38, pp. 342–348.
Likhoshwai, E.V., Sorokovikova, E.G., Bel’kova, N.L., Belykh, O.I., Titov, A.T., Sakirko, M.V., and Parfenova, V.V., Silicon Mineralization in the Culture of Cyanobacteria from Hot Springs, Doklady AN, 2006, vol. 407, no. 4, pp. 556–560 [Doklady Biol. Sci. (Engl. Transl.), vol. 407, pp. 201–204].
Schönhuber, W., Zarda, B., Eix, S., Rippka, R., Herdman, M., Ludwig, W., and Amann, R., In situ Identification of Cyanobacteria with Horseradish Peroxidase-Labeled, RRNA-Targeted Oligonucleotide Probes, Appl. Environ. Microbiol., 1999, vol. 65, pp. 1259–1267.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.G. Sorokovikova, I.V. Tikhonova, O.I. Belykh, I.V. Klimenkov, E.V. Likhoshwai, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 3, pp. 412–420.
Rights and permissions
About this article
Cite this article
Sorokovikova, E.G., Tikhonova, I.V., Belykh, O.I. et al. Identification of two cyanobacterial strains isolated from the Kotel’nikovskii hot spring of the Baikal rift. Microbiology 77, 365–372 (2008). https://doi.org/10.1134/S002626170803017X
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S002626170803017X