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Assembly of LH2 light-harvesting complexes in Rhodopseudomonas palustris cells illuminated by blue and red light

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Abstract

We investigated the formation of the B800-850 complex in cells of the bacterium Rhodopseudomonas palustris AB illuminated by red and blue light under anaerobic growth conditions. Under red illumination, the B800-850 complex was assembled with a reduced absorption band at 850 nm. The results of re-electrophoresis of the B800-850 complex and oxidation in the presence of potassium iridate suggest its heterogeneity. It may be a mixture of two complexes (B800 and B800-850). The B800-850 complex lacks the capacity for conformational transitions if assembled under blue illumination. Accordingly, the light-harvesting complex assembled in the blue light contains polypeptides that are not synthesized under normal conditions or at increased or decreased light intensities. The mechanism of regulation of the synthesis of the polypeptides of light-harvesting the B800-850 complex and its dependence on the spectral composition of the light is discussed.

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References

  1. Erokhin, Yu.E. and Moskalenko, A.A., Characterization of the Proteins of the Chromatium Pigment-Lipoprotein Complexes (Number of Chains and Molecular Weights), Dokl. Akad. Nauk SSSR, 1973, vol. 212, pp. 495–498.

    PubMed  CAS  Google Scholar 

  2. Zuber, H. and Cogdell, R.J., Structure and Organization of Purple Bacterial Antenna Complexes, in Anoxygenic Photosynthetic Bacteria, Blankenship, R.E., Madigan, M.T., and Bauer, C.E., Eds., Dordrecht: Kluwer, 1995, pp. 315–348.

    Google Scholar 

  3. Roszak, A.W., Howard, T.D., Southall, J., Gardiner, A.T., Law, C.J., Isaacs, N.W., and Cogdell, R.J., Crystal Structure of the RC-LH1 Core Complex from Rhodopseudomonas palustris, Science, 2003, vol. 302, pp. 1969–1972.

    Article  PubMed  CAS  Google Scholar 

  4. McDemott, G., Princ, S.M., Freer, A.A., Hawthornthwaite-Lawless, A.M., Papiz, M.Z., Cogdell, R.J., and Isaacs, N.W., Crystal Structure of an Integral Membrane Light-Harvesting Complex from Photosynthetic Bacteria, Nature, 1995, vol. 374, pp. 517–521.

    Article  Google Scholar 

  5. Prokhorenko, I.R., Makhneva, Z.K., and Erokhin, Yu.E., Effect of Different Light Intensities on the Organization of the Rhodopseudomonas palustris AB Photosynthetic Apparatus, Biol. Membrany, 1991, vol. 8, pp. 476–481.

    CAS  Google Scholar 

  6. Evans, M.B., Hawthornthwaite, A.M., and Cogdell, R.J., Isolation and Characterization of the Different B800-850 Light-Harvesting Complexes from Low-and High-Light Grown Cells of Rhodopseudomonas palustris, Strain 2.1.6, Biochim. Biophys. Acta, 1990, pp. 71–76.

  7. Fowler, G.J. and Hunter, C.N., The Synthesis and Assembly of Functional High and Low Light LH2 Antenna Complexes from Rhodopseudomonas palustris in Rhodobacter sphaeroides, J. Biol. Chem., 1996, vol. 271, pp. 13356–13361.

    Article  PubMed  CAS  Google Scholar 

  8. Tharia, H.A., Nightingale, T.D., Papiz, M.Z., and Lawless, A.M., Characterization of Hydrophobic Peptides by Rp-HPLC from Different Spectral Forms of LH2 Isolated from Rps. palustris, Photosynth. Res., 1999, vol. 61, pp. 157–167.

    Article  CAS  Google Scholar 

  9. Gall, A. and Robert, B., A Structural Characterisation of the Different Peripheral Light-Harvesting Complexes from High-and Low-Light Grown Cells from Rhodopseudomonas palustris, Strain 2.6.1, Biochemistry, 1999, vol. 38, pp. 5185–5190.

    Article  PubMed  CAS  Google Scholar 

  10. Braatsch, S., Gomelsky, M., Kuphal, S., and Klug, G., A Single Flavoprotein, AppA, Integrates Both Redox and Light Signals in Rhodobacter sphaeroides, Mol. Microbiol., 2002, vol. 45, pp. 827–836.

    Article  PubMed  CAS  Google Scholar 

  11. Braatsch, S., Moskvin, O.V., Klug, G., and Gomelsky, M., Responses of the Rhodobacter sphaeroides Transcriptome To Blue Light Under Semiaerobic Conditions, J. Bacteriol., 2004, vol. 186, pp. 7726–7735.

    Article  PubMed  CAS  Google Scholar 

  12. Giraud, E., Zappa, S., Vuillet, L., Adriano, J.-M., Hannibal, L., Fardoux, J., Berthomieu, C., Bouyer, P., Pignol, D., and Vermeglio, A., A New Type of Bacteriophytochrome Acts in Tandem with a Classical Bacteriophytochrome To Control the Antennae Synthesis in Rhodopseudomonas palustris, J. Biol. Chem., 2005, vol. 280, pp. 32389–32397.

    Article  PubMed  CAS  Google Scholar 

  13. Anderson, S., Dragnea, V., Masuda, S., Ybe, J., Moffat, K., and Bauer, C., Structure of a Novel Photoreceptor, the BLUF Domain of AppA from Rhodobacter sphaeroides, Biochemistry, 2005, vol. 44, pp. 7998–8005.

    Article  PubMed  CAS  Google Scholar 

  14. Jung, A., Domratcheva, T., Tarutina, M., Wu, Q., Ko, W.-H., Shoeman, R.L, Gomelsky, M., Gardner, K.H., and Schlichting, I. Structure of a bacterial BLUF photoreceptor: Insights into blue light-mediated signal transduction, Proc. Natl. Acad. Sci. USA, 2005, vol. 102, pp. 12350–12355.

    Article  PubMed  CAS  Google Scholar 

  15. Kort, R., Crielaard, W., Spudich, J.L., and Hellingwerf, K.J., Color-Sensitive Motility and Methanol Release Responses in Rhodobacter sphaeroides, J. Bacteriol., 2000, vol. 182, pp. 3017–3021.

    Article  PubMed  CAS  Google Scholar 

  16. Jiang, Z.Y., Swem, L.R., Rushing, B.G., Devanathan, S., Tollin, G., and Bauer, C.E., Bacterial Photoreceptor with Similarity To Photoactive Yellow Protein and Plant Phytochromes, Science, 1999, vol. 285, pp. 406–409.

    Article  PubMed  CAS  Google Scholar 

  17. Kyndt, J.A., Meyer, T.E., and Cusanovich, M.A., Photoactive Yellow Protein, Bacteriophytochrome, and Sensory Rhodopsin in Purple Phototrophic Bacteria, Photochem. Photobiol. Sci., 2004, vol. 3, pp. 519–530.

    Article  PubMed  CAS  Google Scholar 

  18. Giraud, E., Zappa, S., Jaubert, M., Hannibal, L., Fardoux, J., Adriano, J.-M., Bouyer, P., Genty, B., Pignol, D., and Vermeglio, A., Bacteriophytochrome and Regulation of the Synthesis of the Photosynthetic Apparatus in Rhodopseudomonas Palustris: Pitfalls of Using Laboratory Strains, Photochem. Photobiol. Sci., 2004, vol. 3, pp. 587–591.

    Article  PubMed  CAS  Google Scholar 

  19. Larimer, F.W., Chain, P., Hauser, L., Lamerdin, J., Malfatti, S., Do, L., Land, M.L., Pelletier, D.A., Beatty, J.T., Lang, A.S., Tobita, F.R., Gibson, J.L., Hanson, T.E., Bobst, C., Torres, J.L., Peres, C., Harrison, F.H., Gibson, J., and Harwood, C.S., Complete Genome Sequence of the Metabolically Versatile Photosynthetic Bacterium Rhodopseudomonas palustris, Nature Biotechnol., 2004, vol. 22, pp. 55–61.

    Article  CAS  Google Scholar 

  20. Moskalenko, A.A., Kuznetsova, N.Yu., Erokhin, Yu.E., and Toropygina, O.A., Organization and Conformational Transitions in the B800-850 Complex from Chromatium minutissimun, Biokhimiya, 1996, vol. 61, pp. 429–439.

    CAS  Google Scholar 

  21. Moskalenko, A.A. and Erokhin, Yu.E., Isolation of Pigment-Lipoprotein Complexes from Purple Bacteria by Preparative Electrophoresis in Polyacrylamide Gel, Mikrobiologiya, 1974, vol. 43, pp. 654–655.

    CAS  Google Scholar 

  22. Moskalenko, A.A., Britton, G., Konnor, A., Iang, A., and Toropygina, O., Composition of Carotenoids in the Chromophores and Pigment-Protein Complexes Isolated from Chromatium minutissimum Cells Grown in the Presence of diphenylamine, Biol. Membr., 1991, vol. 8, pp. 249–260.

    CAS  Google Scholar 

  23. Makhneva Z. and Moskalenko, A., Pigment-Protein Complexes from the Now Sulfur Photosynthetic Bacterium Ectothiorhodosinus mongolicum Strain M9 with Normal and Inhibited Carotenoid Synthesis, Biol. Membr., 2004, vol. 191.

  24. Moskalenko, A.A., Pigment-Protein Complexes and Their Interaction in the Structures of Bacterial and Plant Photosynthetic Apparatus, Doctoral (Biol.) Dissertation, as a report, IPFS RAN. Pushchino, 1993.

  25. Razjivin, A.P., Moskalenko, A.A., and Novoderezhkin, V.I., A Model of Spectral Canges of the B800-850 Complex from Chromatium minutissimum Induced by Detergent Treatment, IUBMB Life, 2000, vol. 50, pp. 115–117.

    PubMed  CAS  Google Scholar 

  26. Fowler, G.J., Visschers, R.W., Grift, G.G., Grondelle, R., and Hunter, C.N., Genetically Modified Photosynthetic Antenna Complexes with Blueshifted Absorbance Bands, Nature, 1992, vol. 335, pp. 848–850.

    Article  Google Scholar 

  27. Moskalenko, A.A., Makhneva, Z.K., Zhuravleva, Z.A., and Erokhin, Yu.E., Some Spectral Characteristics of Pigment-Protein Complexes and Their Interaction in Membranes of Thiorhodospira sibirica, Dokl. Akad. Nauk, 2002, vol. 382, pp. 836–839 [Dokl. Biochem. Biophys. (Engl. Transl.), vol. 382, pp. 63–66].

    Google Scholar 

  28. Pischal’nikov, R.Yu., Moskalenko, A.A., and Razzhivin, A.P., Hypothesis on the Origin of Three Bands in Absorption Spectrum of the New Type LH2 Complex (B800-830-850) from Thiorhodospira sibirica, Biol. Mem., 2003, vol. 20, pp. 386–394.

    Google Scholar 

  29. Giraud, E., Fardoux, J., Fourrier, N., Hannibal, L., Genty, B., Bouyer, P., Dreyfus, B., and Vermeglio, A., Bacteriophytochrome Controls Photosystem Synthesis in Anoxygenic Bacteria, Nature, 2002, vol. 417, pp. 202–205.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Moscow, Russia

    Z. K. Makhneva, Yu. E. Erokhin & A. A. Moskalenko

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  1. Z. K. Makhneva
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  2. Yu. E. Erokhin
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  3. A. A. Moskalenko
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Correspondence to A. A. Moskalenko.

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Original Russian Text © Z.K. Makhneva, Yu.E. Erokhin, A.A. Moskalenko, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 3, pp. 386–395.

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Makhneva, Z.K., Erokhin, Y.E. & Moskalenko, A.A. Assembly of LH2 light-harvesting complexes in Rhodopseudomonas palustris cells illuminated by blue and red light. Microbiology 77, 339–347 (2008). https://doi.org/10.1134/S0026261708030144

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