Skip to main content
SpringerLink
Log in

Optimization of culture medium and growth conditions for production of L-arabinose isomerase and D-xylose isomerase by Lactobacillus bifermentans

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

Lactobacillus bifermentans was used to produce the intracellular enzymes L-arabinose isomerase and D-xylose isomerase. Various factors of cultivation (temperature, pH, and incubation period) and culture medium composition (mineral salts, carbon source, and nitrogen source) were studied to select the conditions that maximize production of these enzymes. Arabinose isomerase and xylose isomerase activities were 9.4 and 7.24 U/ml, respectively. They were highest at 9 h of cultivation in the optimized medium, 1.6 times higher than that in the basic MRS broth. The optimal medium composition and cultivation conditions were determined. For optimal growth, the strain required Tween 80 (1 g/l) and a source of inorganic nitrogen (e.g., ammonium citrate). The bacterium had no requirement for sodium acetate for either growth or production of isomerases. The production rate of enzymes was increased when metal ions were added, primarily manganese (2.5 mM).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sun, X.F., Xu, F., Sun, R.C., Geng, Z.C., Fowler, P., and Baird, M.S., Characteristics of Degraded Hemicellulosic Polymers Obtained from Steam Exploded Wheat Straw, Carbohydr. Polym., 2005, vol. 60, pp. 15–26.

    Article  CAS  Google Scholar 

  2. Sun, R.C. and Tomkinson, J., Characterization of Hemicelluloses Obtained by Classical and Ultrasonically Assisted Extractions from Wheat Straw, Carbohydr. Polym., 2002, vol. 50, pp. 263–271.

    Article  CAS  Google Scholar 

  3. Sun, R.C. and Hughes, S., Fractional Extraction and Physico-Chemical Characterization of Hemicelluloses and Cellulose from Sugar Beet Pulp, Carbohydr. Polym., 1998, vol. 36, pp. 293–299.

    Article  CAS  Google Scholar 

  4. Kim, B.C., Lee, Y.H., Lee, H.S., Lee, D.W., Choe, E.A., and Pyun Y.R., Cloning, Expression and Characterization of L-Arabinose Isomerase from Thermotoga neapolitana: Bioconversion of D-Galactose to D-Tagatose Using the Enzyme, FEMS Microbiol. Lett., 2002, vol. 212, pp. 121–126.

    PubMed  CAS  Google Scholar 

  5. Wovcha, M.G., Steuerwald, D.L., and Brooks, K.E. Amplification of D-Xylose and D-Glucose Isomerase Activities in Escherichia coli by Gene Cloning, Appl. Environ. Microbiol., 1983, vol. 45, no. 4, pp. 1402–1404.

    PubMed  CAS  Google Scholar 

  6. Richard, P., Verho, R., Putkonen, M, Londesborough, J., and Penttila, M., Production of Ethanol From L-arabinose by Saccharomyces cerevisiae Containing a Fungal L-Arabinose Pathway, FEMS Yeast Res., 2003, pp. 85–189.

  7. Patrick, J.W., and Lee, N., Purification and Properties of an L-Arabinose Isomerase from Escherichia coli, J. Biol. Chem., 1968, vol. 243, pp. 4312–4318.

    PubMed  CAS  Google Scholar 

  8. Bothast, R.J., Nichols, N.N. and Dien, B.S. Fermentation with New Recombinant Organisms, Biotechnol. Prog., 1999, vol. 15, pp. 867–875.

    Article  PubMed  CAS  Google Scholar 

  9. Zhang, M., Eddy, C, Deanda, K., Finkestein, M, and Picataggio, S., Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis, Science, 1995, vol. 267, pp. 240–243.

    Article  PubMed  CAS  Google Scholar 

  10. Deanda, K., Zhang, M., Eddy, C., and Picataggio, S., Development of an Arabinose-Fermenting Zymomonas mobilis Strain by Metabolic Pathway Engineering, Appl. Environ. Microbiol., 1996, vol. 62, no. 12, pp. 4465–4470.

    PubMed  CAS  Google Scholar 

  11. Dien, B.S., Cotta, M.A., and Jeffries, T.W., Bacteria Engineered for Fuel Ethanol Production: Current Status, Appl. Microbiol. Biotechnol, 2003, vol. 63, pp. 256–266.

    Article  Google Scholar 

  12. Chandrakant, P. and Bisaria, V.S., Simultaneous Bioconversion of Glucose and Xylose to Ethanol by Saccharomyces cerevisiae in the Presence of Xylose Isomerase, Appl. Microbiol. Biotechnol., 2000, vol. 53, pp. 301–309.

    Article  PubMed  CAS  Google Scholar 

  13. Lee, C.Y., Bhatnagar, K., Saha, B.C., Lee, Y.E., and Takagi, M., Imanaka, T., Bagdasarian, M., and Zeikus, J.G., Cloning and Expression of the Clostridium thermosulfurogenes Glucose Isomerase Gene in Escherichia coli and Bacillus subtilis, Appl. Environ. Microbiol., 1990, vol. 56, no. 9, pp. 2638–2643.

    PubMed  CAS  Google Scholar 

  14. Borgi, A., Srih-Belguith, K., Ben Ali, M., Mezghani, M., Tranier, S., Haser, R., and Bejar, S., Glucose Isomerase of the Streptomyces sp. SK Strain: Purification, Sequence Analysis and Implication of Alanine 103 Residue in the Enzyme Thermostability and Acidotolerance, Biochimie, 2004, vol. 86, pp. 561–568.

    Article  PubMed  CAS  Google Scholar 

  15. Pawar, S.A. and Deshpande, V.V., Characterization of Acid-Induced Unfolding Intermediates of Glucose/Xylose Isomerase, Eur. J. Biochem., 2000, vol. 267, pp. 6331–6338.

    Article  PubMed  CAS  Google Scholar 

  16. Bhosale, S.H., Rao, M.B., and Deshpande, V.V., Molecular and Industrial Aspects of Glucose Isomerase, Microbiol. Rev., 1996, vol. 60, no. 2, pp. 280–300.

    PubMed  CAS  Google Scholar 

  17. Deshmukh, S.S., Deshpande, M.V., and Shankar, V., Medium Optimization for the Production of Glucose Isomerase from Thermophilic Streptomyces thermonitrificans, World J. Microbiol. Biotechnol., 1994, vol. 10, pp. 264–267.

    Article  CAS  Google Scholar 

  18. Chauhan, K., Trivedi, U., and Patel, K.C., Statistical Screening of Medium Components by Plackett-Burman Design for Lactic Acid Production by Lactobacillus sp. KCP01 Using Date Juice, Bioressour. Technol., 2006 (in press).

  19. Lee, D.W., Choe, E.A., Kim, S.B., Eom, S.H., Hong, Y.H., Lee, S.J., Lee, H.S., Lee, D.Y., and Pyun, Y.R., Distinct Metal Dependence for Catalytic and Structural Functions in the L-Arabinose Isomerases from the Mesophilic Bacillus halodurans and the Thermophilic Geobacillus stearothermophilus, Arch. Biochem. Biophys., 2005, vol. 434, pp. 333–343.

    Article  PubMed  CAS  Google Scholar 

  20. Lee, M.T., Chen, W.C., and Chou, C.C., Medium Improvement by Orthogonal Array Designs for Cholesterol Oxidase Production by Rhodococcus equi, No. 23, Process Biochem., 1997, vol. 32, pp. 697–703.

    Article  CAS  Google Scholar 

  21. Bakhtiari, M.R., Faezi, M.G., Fallahpour, M., Noohi, A., Moazami, N., and Amidi, Z., Medium Optimization by Orthogonal Array Designs for Urease Production by Aspergillus niger PTCC5011, Process Biochem., 2006, vol. 41, pp. 547–551.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de microbiologie industrielle UMR FARE 614, Université de Reims Champagne Ardenne, Moulin de la Housse, B.P. 1039, 51687, Reims cedex 2, France

    S. Givry & F. Duchiron

Authors
  1. S. Givry
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Duchiron
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Givry.

Additional information

The text was submitted by the authors in English.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Givry, S., Duchiron, F. Optimization of culture medium and growth conditions for production of L-arabinose isomerase and D-xylose isomerase by Lactobacillus bifermentans . Microbiology 77, 281–287 (2008). https://doi.org/10.1134/S0026261708030053

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261708030053

Keywords

Search

Navigation